合生素对肉仔鸡免疫器官指数的影响

为探讨合生素对肉仔鸡免疫器官指数的影响,选取1日龄肉仔鸡280只进行试验,按体重随机分成7个组(每组5个重复,每个重复8只鸡)。对照组饲喂基础日粮,试验组饲喂在基础日粮中分别添加合生素Ⅰ、Ⅱ、Ⅲ及其添加水平0.2%和0.3%,试验期42 d。结果表明,合生素的添加显著降低了(P<0.05)肉仔鸡的脾脏和法氏囊免疫器官指数,也使胸腺免疫器官指数呈下降的趋势,但肉仔鸡的生长、健康状况均处于良好的发展状态。结果提示合生素能增强肉仔鸡免疫与抗应激能力。     

青海绵羊红细胞钾型的研究

采用火焰光度法对青海省8个品种绵羊的红细胞钾型多态性特征进行了调查研究。结果发现,被检的8个品种绵羊均有高钾(HK)和低钾(LK)两种表型而呈现多态性,其中欧拉羊、藏羊、小尾寒羊以HK型为优势表型,新疆细毛羊、青海细毛羊、青海半细毛羊以LK型为优势表型;绵羊的红细胞钾型没有性别差异;红细胞钾型与生产性状指标间没有显著性关系;在被检的15项血液指标中,除WBC,BK值和EK值差异显著(P<0.01)以外,其他血液指标没有型间差异(P>0.05);不同红细胞钾型绵羊的HB型分布非常相似(P>0.05)。     

Physiologic and biochemical measurements and response to noxious stimulation at various concentrations of MS‐222 in Koi (Cyprinus carpio)

ObjectiveTo evaluate the physiological effect and response to noxious stimulation at five concentrations of MS-222 in koi (Cyprinus carpio).Study designProspective experimental study.AnimalsTwenty-one healthy adult unknown sex koi fish weighing mean 450 ± SD 120 g.MethodsEach fish was exposed to five different concentrations of MS-222 (50, 70, 110, 150 and 190 mg L^?1) in a random sequence during the same anaesthetic event. For each concentration of MS-222, vital functions such as heart rate (HR) (via Doppler) and opercular rate (OpR) were recorded after a standardized induction period. Response to two noxious stimuli in the form of haemostat clamp pressure applied on the tail and the lip was evaluated, and blood was drawn to measure biochemical and blood gas values.ResultsDecrease in response to noxious stimulation with an increase of MS-222 concentration both for the lip (p = 0.0027) and the tail (p < 0.0001) stimulus was observed. Biochemical values were unaffected by the concentration of MS-222 with the exception of lactate concentration which was weakly correlated with the duration of anaesthesia (r = 0.31, p < 0.001) and the number of times the fish was clamped or bled prior to sampling (r = 0.23, p < 0.001). Opercular rate decreased with the increase in anaesthetic concentration, and HR was not affected.Conclusions and clinical relevanceOur results indicated a decrease in response to stimulus and a decrease in OpR that were associated with increased concentrations of MS-222. This may assist in establishing anaesthetic protocols using MS-222 in fish and supports the use of supramaximal pressure stimuli to teleost fish under variable MS-222 concentrations as a model for future studies.     

GFP和YFP小鼠生长繁殖性能及血液生化指标的研究

在SPF级环境下,对神经系统转GFP和YFP基因荧光小鼠的生长繁殖性能和血液生化指标进行比较研究。选择10周龄GFP和YFP小鼠各30只（雌雄各半）,采取近亲交配繁殖（雌雄1：1）,并统计其生长繁殖性能,用全自动生化分析仪测定小鼠血清中15项生化正常指标。小鼠体重增长在雌雄间有差异而品种间无差异;绝大多数血液生化指标在雌雄间和品种间均无差异。两种小鼠的体格大小相当,产仔能力YFP小鼠强于GFP小鼠,正常血液生化指标主要受遗传基因控制。     

Effect of Ctenocephalides felis strongylus infestation on the performance of West African dwarf sheep and goats

Summary

A study of Ctenocephalides fells strongylus and of its effect on the performance of sheep and goats was made.

Fleas showed more affinity for sheep than goats. There was a high concentration between lamb and kid mortality and the degree of flea infestation. The clinical effects of flea infestation are highlighted. The factors responsible for flea infestation and its control are discussed.     

Autologous point‐of‐care cellular therapies variably induce equine mesenchymal stem cell migration,proliferation and cytokine expression

Reasons for performing study: Autologous cellular therapy products including adipose‐derived stromal vascular fraction (SVF), bone marrow mononuclear cells (BMMNs), cord blood mononuclear cells (CBMNs) and platelet rich plasma are options for treatment of acute orthopaedic lesions while mesenchymal stem cells (MSCs) are culture expanded. These products may contribute to healing by secreting matrix proteins or growth factors, but they may also act on endogenous MSCs to facilitate healing. Objectives: To determine the effects of cell therapy products on MSCs function in vitro. The hypothesis was that cell therapy products promote MSCs functions including proliferation, migration and mediator release. Methods: Fat, bone marrow (BM), cord blood and platelets were obtained from 6 Quarter Horses. The BM‐MSCs and their autologous cell therapy products were co‐incubated in transwells. Mesenchymal stem cells proliferation, migration, gene expression and cytokine concentrations were determined. Results: All cell therapy products increased MSCs proliferation, but SVF induced significantly more proliferation than any other product. Also SVF elicited more MSCs chemotaxis and, along with BMMNs, significantly more MSCs chemoinvasion. Cord blood mononuclear cells stimulated MSCs to produce high concentrations of interleukin‐6 (IL‐6), transforming growth factor‐β1 (TGF‐β1), and prostaglandin E₂ (PGE₂). Stromal vascular fraction and platelet lysate did not stimulate MSCs but SVF and platelet lysate themselves contained high concentrations of PGE₂ and IL‐6 (SVF) and TGF‐β1 (platelet lysate). Conclusions: Autologous cell products variably stimulate MSCs functions with 2 primary patterns apparent. Products either contained preformed mediators that may have intrinsic healing function, or products stimulated MSCs to secrete mediators. Potential relevance: The specific clinical indications for these products may differ to include administration as a sole treatment modality prior to MSCs injection for intrinsic cell and cytokine activity (i.e. SVF) or administration concurrently with MSCs to activate MSCs for treatment of chronic lesions (i.e. CBMNs).     

Detection of bovine herpesvirus type 4 antibodies and bovine lymphotropic herpesvirus in New Zealand dairy cows

AIM: To detect the presence of bovine herpesvirus (BoHV) type 4 in New Zealand dairy cows with clinical metritis.

METHODS: Serum samples taken from 92 dairy cows with clinical metritis, each from a different farm, were tested for the presence of antibodies against BoHV-4 using a commercially available, indirect ELISA. Peripheral blood mononuclear cells (PBMC) were collected from 10 BoHV-4 seropositive cows, and PBMC were examined by a pan-herpesvirus nested PCR to detect herpesvirus. PCR products were sequenced directly and a proportion of the PCR products were cloned and sequenced to identify the virus present.

RESULTS: Antibodies to BoHV-4 were detected in 23/92 (25%) serum samples. The pan-herpesvirus PCR was positive in 8/10 PBMC samples. Cloning and sequencing identified that all of the eight PCR-positive PBMC contained bovine lymphotropic herpesvirus (BLHV); no BoHV-4 DNA was detected.

CONCLUSIONS: This study reports the finding of the presence of apparent antibodies to BoHV-4, and BLHV DNA in New Zealand dairy cows affected by metritis.

CLINICAL RELEVANCE: Bovine herpesvirus type 4 and BLHV are reported to have the potential to cause reproduction failure in cows. This is the first report of apparent BoHV-4 antibodies, and BLHV in New Zealand. The importance and epidemiology of these viruses in cattle in New Zealand requires further investigation.     

水分胁迫对甜椒结果期生理生化指标的影响

在控水条件下,以中椒7号为试验材料,对结果初期、结果盛期和结果末期植株的根系活力、叶绿素、MDA、可溶性糖含量等进行了测定。结果显示：相同控水条件下,结果初期和结果末期MDA含量升高显著,结果盛期则增幅较小,且结果盛期较其他2个时期可溶性糖含量、可溶性蛋白含量升高较多,根冠比变化较大。这说明结果初期和结果末期对水分处理较敏感,不适宜进行控水,而结果盛期内,中椒7号的生理调节能力较其他2个时期强,适当水分胁迫处理不会使植物受到伤害。因此,建议在结果盛期进行适度控水来提高甜椒的水分利用效率。     

国医大师段富津教授治疗胸痹心痛方剂配伍规律研究

目的 分析国医大师段富津教授治疗胸痹心痛用药方剂配伍规律。方法 基于SPSS Modeler数据挖掘软件,利用Apriori算法,对417例胸痹心痛临床跟诊病例,治疗用药的配伍规律进行分析,得出药物之间的关联规则。结果 在分析结果中得出胸痹心痛临床常见证型为气虚血瘀证与气滞血瘀证,常用的药物关联组合为当归和丹参,川芎和丹参,瓜蒌和郁金,黄芪和人参;当归、郁金和丹参,黄芪、人参和当归,川芎、郁金和丹参等。结论 段富津教授在胸痹心痛的治疗用药过程之中的方剂配伍常以补气、活血、宽胸理气的中药组合为主。