基于逆周期的主权信用评级指标体系研究

现行国际主权信用评级呈现出明显的顺周期特征而广受诟病。在对评级指标体系优化的基础上，采用90个主权国家2005~2012年经济数据进行面板随机效应和门限效应回归，对主权信用评级决定模型进行估计，运用欧债危机发生前后的数据进行模拟评级，结果表明：优化后的指标体系显示出较好的逆周期特征。     

Effect of the particle size on the ammonia removal rate and the bacterial community composition of bioflocs

The total ammonia nitrogen (TAN) removal efficiency and bacterial community composition of bioflocs with <50-μm particle size, > 50-μm particle size and un-sieved bioflocs were investigated in the current study. The initial ratio of dissolved organic carbon to TAN (DOC/TAN) in the three groups were about 14:1. No significant difference was found in the removal rate of TAN, average concentrations of TAN and nitrite nitrogen among the three groups (P > 0.05). The C/N (w/w) ratio of the > 50-μm bioflocs was significantly higher than those of the other groups. No significant differences were found in the crude protein content in the bioflocs among the three groups. The development of the bacterial community compositions of the bioflocs was analyzed by Illumina MiSeq sequencing analyses. Most OTUs were shared among the three groups at all the sampled time points. With the increase in the relative abundance of phylum Firmicutes, that of phylum Proteobacteria, Chorolexi, and Bacteroidetes decreased in all the three groups. The phylum Firmicutes and genus Bacillus were predominant in all the sampled time points. At the end of the experiment, genus Bacillus accounted for 81% in the < 50-μm group, 82% in the > 50-μm group, and 75% in the un-sieved group.     

利用CRISPR/Cas9和λ-Red级联技术构建产肠毒素大肠杆菌LT敲除菌株

本研究旨在利用CRISPR/Cas9和λ-Red级联的技术对产肠毒素大肠杆菌（enterotoxigenic Escherichia coli，ETEC）K88的热不稳定性肠毒素（heat-labile toxin，LT）基因进行无痕敲除并获得K88 LT^-缺陷菌株。通过序列比对获取LT两端同源序列，并构建包含LT边界、氯霉素筛选标记、sgRNA和LT同源臂的供体片段；将供体片段转化至ETEC K88，同时分别利用λ-Red同源重组系统和CRISPR/Cas9基因编辑系统，对LT基因进行敲除；通过PCR验证获得了K88 LT^-缺陷菌株，并通过试验测定了敲除菌株的溶血能力和生长曲线。结果显示，λ-Red同源重组系统可成功地将LT基因替换为相应的供体片段，CRISPR/Cas9基因编辑系统可高效地对筛选标记进行删除，最终通过λ-Red和CRISPR/Cas9结合的基因编辑系统可成功对ETEC K88的LT基因进行无痕敲除。体外试验结果表明，K88 LT^-缺陷菌株的溶血能力丧失，并且生长速度比野生型菌株减缓，LT可能和ETEC K88的致病能力和生长性能有关。表明λ-Red和CRISPR/Cas9级联的基因敲除方法可用于LT毒素基因及其他一些大肠杆菌基因的敲除。K88 LT^-缺陷菌株的构建为下一步研究LT毒素的致病机制奠定基础。     

北票市畜牧技术推广体系改革与建设项目实施效果及建议

深化基层畜牧技术推广体系改革与建设，旨在提升基层畜牧技术推广体系公共服务能力。北票市畜牧技术推广站通过一系列措施，建立健全新型基层畜牧技术推广服务体系。基层畜牧技术推广体系改革与建设推进了体制机制创新，完善了工作机制，夯实了人才基础，提高了服务效能。通过两年的实践证明，基层畜牧技术推广体系改革与建设项目实施，有效解决了畜牧技术推广服务体系“最后一公里”问题。     

基于MaxEnt模型的可可潜在适宜分布研究

根据野外采样和文献查阅,系统整理了可可的地理分布记录,并利用MaxEnt生态位模型和ArcGIS软件对可可的潜在适宜分布范围进行预测。结果表明:北美洲南部、中南美洲北部、非洲西部、亚洲东南部以及太平洋美拉尼西亚群岛地区均是可可的潜在适宜分布区域。其中,中国海南、台湾南部、云南西双版纳、广东雷州半岛也属于可可的适生范围。经ROC(Receiver operating characteristic)曲线分析法验证,MaxEnt模型的AUC(Area under curve)值为0.977,表明预测结果具有较高的可信度。各环境变量重要性的Jacknife检验表明,极端最低温度、年降雨量、年温度变化范围、最暖季降雨量对可可的潜在分布影响最大。     

基于粒子群算法的海洋平台热介质锅炉PID参数优化

文中采用串级控制作为海洋平台热介质锅炉的控制方案，采用粒子群算法对PID控制器的比例带啄、积分时间Ti进行最优化，从而实现热介质锅炉温度的自动控制。通过粒子群算法进行串级控制系统PID参数优化，提高了控制系统的控制精度、可靠性、稳定性，具有工业应用价值。     

天津市校园快递智能管理系统

随着电子商务的普及化,快递行业飞速发展,快递“最后一公里”的问题成为快递业迅速发展的瓶颈,笔者以天津市高校为研究对象,通过对高校快递问题的分析总结,提出了一些对目前校园里现有的快递智能管理系统的改善建议.     

Dysbiotic gut microbiome: A key element of Crohn's disease

Since the first publication on “regional ileitis”, the relevance of this chronic inflammatory disease condition termed finally as Crohn's disease is continuously increasing. Although we are beginning to comprehend certain aspects of its pathogenesis, many facets remain unexplored. Host's gut microbiota is involved in a wide range of physiological and pathological processes including immune system development, and pathogen regulation. Further, the microbiome is thought to play a key role in Crohn's disease. The presence of Crohn's-associated variants of NOD2 and ATG16L genes appears to be associated not only with alterations of mucosal barrier functions, and bacterial killing, but the gut microbiota, as well, reflecting a potential relationship between the host's genotype and intestinal dysbiosis, involved in disease etiology. This review aims to characterize some exciting new aspect of Crohn's disease pathology, focusing mainly on the role of intestinal microbes, and their interplay with the immune system of the host.     

林业家庭承包与双层经营体制的探讨

通过对林业家庭承包与其他方式承包在立法概念、物权性质、条件设置、流转方式等诸方面的比较分析,阐明农村土地家庭承包赋予农民人均一份长期而有保障的完整永久的林地使用权,促进林业、农村经济发展和社会稳定;而其他方式承包是家庭承包的补充,虽承包经营,但承包期内村集体仍始终享有全部收益权和处置权。落实统分结合双层经营体制,家庭承包应占主导地位。并通过调研实例说明,农民专业合作组织克服了小农经济的弊端,是适应现代林业发展的一种统分结合双层经营体制。     

新时期浙江省农机装备产业发展对策的探讨

为促进浙江省农业机械装备产业又好又快发展,在分析浙江省农机装备产业发展的特色和产业发展中存在的问题基础上,根据当今国内外农机装备与技术发展趋势和国内外市场需求,提出了加快浙江省农机装备产业发展的相关措施与建议。