Enhancement of revegetation on construction fill by fertilizer and topsoil application: Effect on mycorrhizal infection

The influence of topsoil and fertilizer application on denuded road construction sites was evaluated to assess its contribution to improvement of vegetation re-establishment. the study sites were within a mixed hardwood and conifer forest on crushed, unweathered subsurface material with low fertility and low biological activity. Topsoils were removed from the site, stockpiled and reapplied to the site after construction. the effect of topsoil amendment on plant growth, soil fertility, mycorrhizal infection and an index of microbial biomass were measured in field and greenhouse experiments. Plant growth on the topsoil amended field plots were greatly increased relative to treatments with fertilizer but no topsoil. Three years after establishment, dry weight production on the plots without topsoil treatment was about 40 per cent of the plots treated with topsoil. Greenhouse experiments were designed to compare fresh, dried and stockpiled topsoil. These experiments indicated that storage of the harvested topsoil for five months in a stockpile had minor effects on plant growth, soil fertility, mycorrhizal infection and microbial biomass. Topsoil volumes had to exceed 20 per cent of the total soil volume to achieve statistically significant benefits and higher ratios showed greater benefit. the percentage of mycorrhizal infection was greatest in topsoil treatments without fertilizer. the addition of fertilizer increased growth but reduced the percentage of roots forming mycorrhizae. When the total weight of infected roots was calculated, however, infection was found to be greatest with a moderate level of fertilizer (equivalent to approximately 27 kg N ha⁻¹ and 39 kg P ha⁻¹), and was less in both higher fertilizer treatments and in unfertilized treatments. Topsoil amendment increased microbial biomass nitrogen but fertilizer treatment did not.     

复合蛋白对肉牛生产性能和血液指标的影响

西门塔尔杂交公牛36头,随机分为3组,每组12头。对照组使用牛场原有的预混料及其推荐配方,试验组使用复合蛋白替代蛋白质饲料,比例分别为试验1组6%、试验2组10%。分别测定日增重、饲料转化率,血液指标,消化率并进行饲喂效益分析。结果表明:试验1组和试验2组日增重比对照组分别提高2.1%和11.6%,且差异显著(P<0.05);试验1组和试验2组料肉比比对照组分别降低1.9%(P>0.05)和显著降低10.4%(P<0.05);血液指标和养分消化率变化不大,差异不显著(P>0.05);饲喂净效益试验2组比对照组提高71%。说明合理剂量复合蛋白替代日粮中豆粕等蛋白饲料,不但不降低肉牛生产性能,而且还可以提高日增重,降低料肉比,增长效益。     

不同施氮量下南方红壤花生农艺性状、产量及土壤养分的变化

为探讨南方红壤旱地不同施氮量下花生农艺性状、产量和土壤养分的变化,采用田间随机区组试验,以高含氮肥料为材料,设置8个处理:不施氮肥（N₀）、200%施氮量（N_200%）、150%施氮量（N_150%）、100%施氮量（N_100%,纯施氮180.48 kg/hm²）、80%施氮量（N_80%）、60%施氮量（N_60%）、40%施氮量（N_40%）和20%施氮量（N_20%）。结果表明:与N₀处理相比,施氮肥提高了花生荚果产量,增产幅度为12.94%~24.62%;荚果产量、单株果重和饱果率随施氮量的增加而增加;而分枝数和百仁重随施氮量变化不明显;株高和百果重以N_80%效果最佳。土壤碱解氮随施氮量的增加而增加。相关分析结果表明,花生荚果产量与饱果率、有机质和碱解氮呈显著正相关;碱解氮与饱果率和有机质呈显著正相关,而与pH呈显著负相关。由主成分分析结果可知,第1主成分性状,即产量因子,如荚果产量、单株果重和百果重对花生生长有重要的作用。通径分析结果表明,百果重和饱果率通径系数较大,所以在提高花生荚果产量中应重视这2个性状。综上所述,从花生产量、农艺性状和成本考虑,以N_80%处理（纯施氮144.38 kg/hm²）的荚果产量、株高、单株果重、百果重、百仁重表现最佳。     

不同类型水稻品种产量和氮素吸收利用对大气CO2浓度升高响应的差异

目的 探明不同类型水稻品种产量和氮素吸收利用对FACE(大气CO₂浓度增高)响应的差异。方法 以常规粳稻、杂交籼稻、常规籼稻共6个品种为供试材料,研究FACE对不同类型水稻产量、氮素吸收利用的影响。结果 1）FACE处理极显著提高了水稻产量,平均增加24.17%, 常规籼稻增幅最大,FACE和对照均以杂交籼稻最高;2）FACE处理显著增加了单位面积穗数,常规粳稻增幅最大,并显著增加了杂交籼稻和常规籼稻每穗粒数;3）FACE处理显著提高了成熟期吸氮量和氮素籽粒生产效率,成熟期吸氮量平均增加21.23%,杂交籼稻增幅最大, FACE和对照均以常规籼稻最高;氮素籽粒生产效率平均增加7.33%,杂交籼稻增幅最大,FACE和对照均以杂交籼稻最高。成熟期吸氮量对产量促进作用略大于成熟期氮素籽粒生产效率;4）FACE处理降低了植株含氮率,成熟期平均下降0.105个百分点,常规粳稻降幅最大。FACE处理极显著提高植株干物质量,成熟期平均增加23.95%,常规籼稻增幅最大;FACE处理显著提高常规籼稻和杂交籼稻成熟期单穗吸氮量,分别增加10.79%、13.93%,但常规粳稻下降了9.60%;FACE处理显著提高了成熟期群体吸氮强度,平均增加22.29%,杂交籼稻增幅最大。FACE处理对水稻全生育期天数无显著影响;FACE处理显著提高茎鞘、叶片、穗各器官吸氮量,叶片增幅最大,平均增加51.86%,杂交籼稻增幅最大;FACE处理显著提高了不同生育阶段吸氮量,抽穗-成熟阶段增幅最大,平均增加108.90%,杂交籼稻增幅最大;5）植株干物质量、单穗吸氮量、吸氮强度、穗吸氮量、抽穗-成熟阶段吸氮量对成熟期总吸氮量的促进作用分别大于植株含氮率、单位面积穗数、生育天数、茎鞘叶吸氮量、移栽-分蘖和分蘖-抽穗阶段吸氮量;6）FACE处理显著提高了氮肥偏生产力,降低了每百千克籽粒需氮量,前者平均增加24.16%,常规籼稻增加最多;后者平均降低4.7%,常规籼稻降幅最大。结论 FACE处理可显著提高水稻产量和氮素吸收利用效率,但品种间差异较大。     

Relay-intercropping soybean with maize maintains soil fertility and increases nitrogen recovery efficiency by reducing nitrogen input

Optimized nitrogen(N)management can increase N-use efficiency in intercropping systems.Legume-nonlegume intercropping systems can reduce N input by exploiting biological N fixation by legumes.Measurement of N utilization can help in dissecting the mechanisms underlying N uptake and utilization in legume-nonlegume intercropping systems.An experiment was performed with three planting patterns:monoculture maize(MM),monoculture soybean(SS),and maize-soybean relay intercropping(IMS),and three N application levels:zero N(NN),reduced N(RN),and conventional N(CN)to investigate crop N uptake and utilization characteristics.N recovery efficiency and 15N recovery rate of crops were higher under RN than under CN,and those under RN were higher under intercropping than under the corresponding monocultures.Compared with MM,IMS showed a lower soil N-dependent rate(SNDR)in 2012.However,the SNDR of MM rapidly declined from 86.8%in 2012 to 49.4%in 2014,whereas that of IMS declined slowly from 75.4%in 2012 to 69.4%in 2014.The interspecific N competition rate(NCRms)was higher under RN than under CN,and increased yearly.Soybean nodule dry weight and nitrogenase activities were respectively 34.2%and 12.5%higher under intercropping than in monoculture at the beginning seed stage.The amount(Ndfa)and ratio(%Ndfa)of soybean N2 fixation were significantly greater under IS than under SS.In conclusion,N fertilizer was more efficiently used under RN than under CN;in particular,the relay intercropping system promoted N fertilizer utilization in comparison with the corresponding monocultures.An intercropping system helps to maintain soil fertility because interspecific N competition promotes biological N fixation by soybean by reducing N input.Thus,a maize-soybean relay intercropping system with reduced N application is sustainable and environmentally friendly.     

接种根瘤菌对西南地区大豆光合性能和固氮能力的影响

为探讨接种根瘤菌对西南地区大豆固氮能力的有效性,在大田试验条件下,以根瘤菌株 Bradyrhizobium japonicum5038和B. japonicum 5136侵染南黑豆20和南夏豆25,比较两个大豆品种接种后植株的光合特性和叶绿素 含量,考察根瘤数量并测定分析其蔗糖含量、豆血红蛋白含量和固氮酶活性。结果表明：接种后两大豆品种叶片 chla/b值显著下降,叶绿素含量、净光合速率和气孔导度显著增加;根瘤数、单株瘤重、植株地上部和地下部生物量 均显著增加,单个瘤重和根冠比则无显著变化;根瘤内蔗糖含量和豆血红蛋白含量均显著增加,固氮酶活性提高。 不同大豆品种对不同菌株的响应不同,南夏豆25与根瘤菌B.japonicum5136的适配性最好,接种后大豆光合性能和 固氮能力的改善效果明显优于南黑豆20。     

不同控释氮肥运筹对粳稻养分吸收与氮素利用的影响

以绥粳18为供试材料,通过盆栽试验,在一次性施入控释氮肥免追肥条件下,设置不同施肥量(高、中、低、无)和施肥深度(5 cm、10 cm),探讨不同氮肥施用方式对粳稻养分吸收和氮素利用的影响。结果表明,在不同氮肥运筹条件下,粳稻各生育期氮、磷、钾的吸收、转运均存在明显的协同效应,施肥深度5 cm组,随着氮肥施用量的增加,粳稻的氮吸收总量增加,同时可促进磷的吸收,但影响幅度较小。钾变化与氮、磷变化有所不同。施肥深度10cm组,施高量氮肥抑制了粳稻对磷的吸收、减少了对氮的吸收,但有利于提高氮素利用率,对钾的吸收无显著影响。施用树脂包膜控释肥,纯氮用量为153 kg/hm^2、施肥深度5 cm、一次性施肥免追肥可获得最优水稻氮素利用效率并显著提高磷钾肥利用效率。     

施石灰和秸秆还田对双季稻产量和氮素吸收的互作效应

红壤稻田面临土壤酸化和肥力偏低的双重挑战。施石灰和秸秆还田分别是稻田土壤酸化改良和培肥的有效措施,但二者的互作效应尚不清楚。本研究连续4年(2015—2018年)在江西省开展施石灰和秸秆还田双因素田间定位试验,旨在探明施石灰和秸秆还田对红壤双季稻田水稻产量和氮素吸收的互作效应。结果表明,施石灰和秸秆还田均显著提高了早、晚稻的产量和氮素吸收,且二者具显著的协同促进效应。秸秆还田下,施石灰使早稻产量和氮素吸收分别增加10.7%和15.5%;而在秸秆不还田下,增幅仅分别为4.4%和9.7%。秸秆还田下,石灰使晚稻产量和氮素吸收分别提高18.7%和24.6%;但在秸秆不还田下,增幅则分别为10.5%和5.7%。施石灰对早、晚稻产量和氮素吸收的促进效应随试验年限的增加而减弱。石灰对土壤pH值的提升效应随试验年限的延长显著降低。试验4年后,石灰对土壤有机质和全氮含量均无显著影响;秸秆还田显著提高了土壤有机质含量,而对全氮含量无显著影响。因此,秸秆还田配施石灰能够协同实现双季稻增产、土壤酸化改良与培肥。本研究表明在此酸性的红壤双季稻田上每4年左右施用一次石灰为宜。     

江西省水稻蓄雨间歇灌溉模式初探

【目的】探索水稻蓄雨间歇灌溉模式节水减排效益。【方法】以鄱阳湖区双季早晚稻为试验材料,采用大田和测坑试验,研究了水稻蓄雨间歇灌溉模式对灌溉定额、排水定额、降雨有效利用率、产量、稻田水分生产率,以及氮、磷排放量的影响,并与间歇灌溉和常规淹水灌溉试验进行了分析比较。【结果】与淹水灌溉、间歇灌溉相比,蓄雨间歇灌溉灌排水量、灌排次数明显减少。双季早晚稻年平均灌水量分别减少975m^3/hm^2和1251m^3/hm^2,年平均灌水次数分别减少8次和7.5次;年平均排水量分别减少729 m^3/hm^2和893 m^3/hm^2,年平均排水次数分别减少5.8次和3.1次;蓄雨间歇灌溉降雨有效利用率明显提高。早稻降雨利用率分别提高12.40%和9.14%,晚稻分别提高6.84%和6.42%;蓄雨间歇灌溉模式下,双季早晚稻总氮排放量年平均减排7.64 kg/hm^2和3.12 kg/hm^2,减排幅度34.93%和14.26%;双季早晚稻总磷排放量0.180kg/hm^2和0.095kg/hm^2,减排幅度37.25%和70.59%。【结论】蓄雨间歇灌溉模式具有明显的节水、减排和提高降雨有效利用率的效果,在我国南方多雨地区具有较强的推广应用空间。     

光氮互作对芹菜幼苗生长及生理特性的影响

光质和施氮量是影响芹菜生长发育的关键因素,适宜的光氮组合能有效提升芹菜幼苗质量。为优化芹菜工厂化育苗,本试验设置2种光质(白光,W;蓝光,B)和2种施氮量(8mmol/L KNO_3,高氮,H;4mmol/L KNO_3,低氮,L),以WH为对照,研究光氮互作对芹菜幼苗生长、生理代谢和元素积累的影响。结果表明:与WH相比,WL和BH处理的芹菜全株干重分别显著减少43.18%和55.07%,WL处理的叶片和叶柄中硝酸盐、可溶性蛋白质和总游离氨基酸质量分数均显著降低,BH处理的叶片和叶柄中硝酸盐、可溶性蛋白质和矿质元素质量分数显著增加,而叶片中可溶性糖、丙氨酸族和丝氨酸族氨基酸质量分数均显著降低。然而BL处理的芹菜全株干重比WH显著增加32.18%,叶片可溶性蛋白质、叶柄组氨酸(His)和脯氨酸(Pro)质量分数均显著增加。利用隶属函数分析对芹菜幼苗生长发育进行综合评价发现,BL处理表现最优。综上所述,蓝光和低氮组合能促进芹菜干物质积累,提高可溶性蛋白质和游离氨基酸质量分数,进而促进芹菜幼苗生长发育。