Analysis of FMO genes and off flavour in pork

Off flavours in pork sometimes produce tastes such as sourness, fishy, metallic or other non‐typical flavours and are often caused by low pH. Loss of function mutations in flavin containing mono‐oxygenase 3 (FMO3) are known to be associated with a fishy off flavour in both chicken eggs and cow's milk and a similar autosomal recessive disorder is present in humans resulting in a fishy odour. FMO3 is a member of a gene family that is clustered on human chromosome 1. Comparative mapping suggested that FMO3 and the remaining FMO genes (ex. FMO1 and FMO5) might map to the orthologous region on pig chromosome 9 (SSC9) where a quantitative trait locus (QTL) for off flavour was previously identified. Primers were designed to amplify FMO1, FMO3 and FMO5 gene fragments and several SNPs were discovered and genotyping tests developed. The genotypes from the Iowa State University Berkshire × Yorkshire resource population were used to linkage map FMO1 and FMO3 to SSC9 and FMO5 to pig chromosome 4 (SSC4). QTL and associations analyses were performed using the map containing FMO1 and FMO3. Results demonstrated that FMO3 and FMO1 mapped less than 1 cM away from the peak for the off flavour QTL previously detected on SSC9 and provide indications of an association between the FMO3 polymorphism and off flavour in pork.     

Evaluation of association between polymorphism within the thyroglobulin gene and milk production traits in dairy cattle

In dairy cattle, many studies have reported quantitative trait loci (QTL) on the centromeric end of chromosome 14 that affect milk production traits. One of the candidate genes in this QTL region – thyroglobulin (TG) – was previously found to be significantly associated with marbling in beef cattle. Thus, based on QTL studies in dairy cattle and because of possible effects of this gene on fat metabolism, we investigated the association of TG with milk yield and composition in Holstein dairy cattle. A total of 1279 bulls from the Cooperative Dairy DNA Repository Holstein population were genotyped for a single nucleotide polymorphism in TG used previously in beef cattle studies. Analysis of 29 sire families showed no significant association between TG variants and milk production traits. Within‐sire family analysis suggests that TG is neither the responsible gene nor a genetic marker in association with milk production traits.     

Genome-wide Association Study on Alive Litter Size Trait in Bama Xiang Pigs

In order to identify the molecular markers related to alive litter size of Bama Xiang pigs,the genome-wide association study (GWAS) was used to map and screen the candidate genes affecting the alive litter size trait.Ear tissue samples of 297 Bama Xiang pigs with multiple parity records were collected,and DNA was extracted and genotyped by porcine 50K SNP beadchip.After quality control and genotype imputation,the alive litter size of Bama Xiang pigs were GWAS by Tassel.The results showed that the average number born alive per litter of Bama Xiang pigs increased gradually with the increasing of parity in the range of 1-9 parities.A total of 32 816 SNPs were obtained after quality control and filtration.8 SNPs related to alive litter size of Bama Xiang pigs were screened by genome-wide association analysis,which were significant at genome or chromosome level.Based on the enrichment analysis of the coding genes in the region between 500 kb upstream and downstream of the associated significant SNP loci,and the QTL regions and gene functions related to porcine reproductive traits,4 genes (CAPZB,MSH3,CITED2 and HSD17B7) were finally identified to be candidate genes related to alive litter size of Bama Xiang pigs.     

补充青贮玉米对复合稻草块体外发酵特性的影响

应用体外发酵产气技术,研究了青贮玉米以不同比例(0%～100%)替代复合稻草块中稻草对其体外发酵特性的影响。结果表明:随着青贮玉米比例的升高,复合稻草块的24h产气量、产气速度常数、有机物消化率、代谢能呈逐渐上升的趋势(P<0.05)。替代比例低于40%时24h产气量组合效应不显著(P>0.05),替代比例高于40%后出现逐渐增大的正组合效应,当替代比例为50%时,正组合效应值(9.68)显著增加(P<0.05),以后变动幅度不大。综上可见,用青贮玉米替代复合稻草块中40%以上的稻草较为适宜,当替代比例为50%时,就可获得较好的消化率。     

不同氮磷钾组合搭配对佛手茶产量效应的影响初报

以茶叶目标产量为基础，以氮磷钾肥料不同组合搭配进行L9（3^4）正交试验，结果氮磷钾肥取2：1：1水平为最优化配方组合，处理4（A281C2）、处理6（A283C1）较接近最优化配方组合，但经经济效益分析，只有处理6（A283C1）单产582．00kg／667m^2，纯收入2973．59元／667m^2为最佳组合。由此认为在粘质红壤施肥，应以氮肥为主，配合施用速效磷肥，即尿素32．60kg／667m^2、钙镁磷66.18kg／667m^2、氯化钾7．50kg／667m^2，纯N14．996kg／667m^2，N：P2O5：K2O=1：0．75：013为最佳组合较合理。佛手茶是大叶品种，适量增施氮磷肥，有利于促进新梢的生长而提高产量.     

农业信息化多媒体Web数据库构建——以三峡库区为例

三峡库区农业信息化平台的核心是农业信息化多媒体数据库系统的架构。通过对农业信息化平台的需求分析,提出在.NET平台下多媒体数据库的设计思想,阐述了多媒体数据库中主要表的设计和多媒体数据关联。实现了农业信息化平台中表的关联和多媒体Web数据库中数据的查询。     

红树林海岸生物多样性专项库的设计

为了加强对红树林生态环境保护的宣传力度，增强图书馆对外服务的功能，满足对红树林海岸生物多样性信息编目、分类等资料管理需求，采用目前先进的Web技术、数据库技术、数据处理技术构建了红树林海岸生物多样性信息管理系统，较为详细地讨论了构建专项库的意义以及关键技术。     

新图书馆运动

新图书馆运动实现了中国图书馆从藏书楼到近代图书馆的转变,促进了图书馆事业的发展,成立了图书馆协会,形成了图书馆学教育的规模,产生了第一代图书馆学人。     

黎蒴接种菌根菌研究

在清远地区对华南乡土阔叶树种黧蒴接种5个外生菌根(ECM)真菌,造林5个月后观测ECM菌根菌对该树种生长和保存率的影响。结果表明接种ECM菌对藜蒴树高生长和保存率都有不同程度的提高,其中以彩色豆马勃对黎蒴的促生性最好,红菇和正红菇两个ECM菌对藜蒴苗高生长和保存率也有显著或极显著的促进作用,但接种效果不如彩色豆马勃。     

联结度指数构造模型及其应用

植物种间联结关系的研究约有 1 0 0年的历史 ,国内外学者提出了多种公式 :I1 =(ad-bc) (ad bc) (Yule ,1 91 2 ) ,I2 =2a (2a b c) (Dice ,1 94 5 ) ,I3 =(a b) (a c) a(Margalef,1 95 8) ,上述这些公式均没有考虑每一样方的种群数量 ,而只检查种是否出现 ;无疑这些指数受