Oral administration of fasudil hydrochloride suppresses development of experimental autoimmune encephalomyelitis in mice

AIM:To explore the therapeutic effect of fasudil hydrochloride by the oral route on the development of experimental autoimmune encephalomyelitis (EAE) in mice and its possible mechanism. METHODS:The EAE model in female C57BL/6 mice was established by myelin oligodendrocyte glycoprotein 35-55 peptide(MOG35-55) immunization and the immunized mice were randomly divided into saline control group and fasudil intervention group, in which saline and fasudil were administered by the oral route once every day from post-immunization (PI) day 3 to day 27. Clinical score and body weight were recorded every other day. On PI day 28, the spinal cords were obtained for HE and myelin staining. The splenocytes were isolated and the expression of CD16/32, CD206 and interleukin (IL)-10 was analyzed by flow cytometry. The levels of IL-1β and tumor necrosis factor α (TNF-α) were detected by ELISA. RESULTS:Oral administration of fasudil delayed the onset of EAE, and attenuated the myelinoclasis of the model animals and the severity of EAE, accompanied by the phenotypic switch from M1 to M2 macrophages, the inhibition of the proinflammatory cytokine (IL-1β and TNF-α) production and the increase in IL-10 release. CONCLUSION: Oral administration of fasudil exhibits therapeutic effect on the development of EAE possibly through switching M1 macrophages to M2 phenotype and inhibiting inflammatory responses in mice.     

基于J2EE的高职院校教务管理系统的分析与设计

通过对高职院校教务管理系统的功能需求分析与设计,介绍了开发过程所运用的Jsp—servlet—JavvBean关键技术,并分析了J2EE访问SQL Server数据库的方法和技巧。     

建设农业政务网站的实践与体会

通过多年来建设江苏农业网的实践,总结出了办好农业政务网六个方面的体会,即对照测评指标体系,加强网站改造;推进政务公开,加强网上办事;整合信息资源,加强内容建设;突出农业特色,加强信息服务;健全信息队伍,加强业务培训;制定规章制度,加强工作领导.     

我国税收遵从影响因素的实证分析

莎士比亚的《暴风雨》在后殖民批评中被看作是殖民主义寓言,其中普洛士帕罗和卡力班分别是殖民者和被殖民者的象征。这个批评视角的形成与加勒比海地区原殖民地作家对《暴风雨》所进行的解读和挪用具有前因后果的关系。文章一方面从加勒比海地区作家莱明、雷塔马、塞萨尔等人的作品中,看殖民地作家在反抗殖民主义、寻找自我意识的过程中,如何对《暴风雨》和西方学者的研究加以挪用,另一方面回到莎士比亚原著,寻找使挪用成为可能的线索。     

中国渔政管理指挥系统总体解决方案

针对正在建设中的中国渔政管理指挥系统项目,根据渔政管理对指挥和信息的基本需求,对该系统的网络结构、应用软件和系统运行等方面的解决方案进行了研究,系统由渔业船舶管理业务系统、渔政船调度指挥与渔船海上动态监测系统、渔政执法管理信息查询系统和渔业资源环境遥感监测评估服务系统组成,分别采用了计算机网络技术、全球定位系统(GPS)技术、地理信息系统(GIS)技术、网络通讯技术以及遥感信息技术,系统网络结构采用树状和星形相结合的形式,网络各站点的通讯方式通过ISDN、ADSL和PSTN拨号接入,运行方案中各级站点具有明晰的数据流程和业务职责,解决方案符合我国渔政管理需要,技术上具有先进性和实用性的特点。     

Clinical and pharmacokinetic characteristics of intracavitary administration of pegylated liposomal encapsulated doxorubicin in dogs with splenic hemangiosarcoma

BACKGROUND: Canine splenic hemangiosarcoma (HSA) is a fatal malignancy, and most affected dogs die within a few months of diagnosis. Most dogs present with signs from tumor rupture, resulting in hemoabdomen and intra-abdominal dissemination. The abdomen is also the main site of disease recurrence. HYPOTHESIS: Intraperitoneal (IP) administration of doxorubicin will delay or prevent intra-abdominal tumor recurrence and prolong survival in dogs with HSA. ANIMALS: Fourteen dogs with splenic HSA. METHODS: A prospective, unmasked, uncontrolled clinical trial. After staging of disease status and splenectomy, pegylated liposomal encapsulated doxorubicin was administered intraperitoneally (1 mg/kg body weight) every 3 weeks for 4 cycles. All dogs were monitored for recurrence of HSA. Samples of plasma and abdominal fluid were collected for measurement of doxorubicin concentration and pharmacokinetic analysis. Nonlinear mixed-effect modeling was used to describe the pharmacokinetics of liposomal doxorubicin administered IP. RESULTS: All 14 dogs died, 12 because of HSA and 2 from other causes. Postmortem examination was performed on 12 dogs. All 12 dogs died because of HSA-related causes and had hepatic metastases and hemoabdomen. The IP-treated dogs had fewer serosal, mesenteric, and omental metastases than historical controls treated with systemic doxorubicin. Results of the postmortem examination and pharmacokinetic analysis confirmed that IP delivery of doxorubicin resulted in an effective drug concentration with a clearance comparable with that after i.v. delivery. CONCLUSIONS AND CLINICAL IMPORTANCE: IP pegylated liposomal encapsulated doxorubicin administration did not prevent intraabdominal recurrence of HSA in dogs.     

谈高校工程项目招投标及管理

阐述招投标的原则、方式、方法,并结合工程管理实践,指出了高校在招投标管理过程中应注意的事项及加大管理力度。     

对地方工科院校工商管理专业大类学生培养的思考

地方工科院校侧重于培养地方应用型人才,其人才培养应当贴近地方经济建设、社会发展和行业需要,培养为地方社会经济发展服务、具有实践能力和创新能力的第一线的应用型人才,工商管理按大类招生培养,培养的学生是厚基础、对专业学习深入了解的交叉复合型的人才。该文对工商管理大类招生的必要性,当前工商管理本科专业人才培养存在的主要问题进行分析,并提出地方工科院校工商管理专业大类培养的建议,以为工商管理专业大类学生培养提供参考。     

试论林业科技档案的利用与知识产权保护

林业科技档案的价值,在于为林业生产活动提供必要的参考和依据。利用好林业科技档案信息资源,可以对提高林业综合生产能力及社会、经济、生态效益,改善人民生态环境等方面起到积极的促进作用。但在林业科技档案的利用过程中,常常出现档案利用与知识产权保护之间的冲突,处理不当则容易导致知识产权的流失或破坏。因此,通过分析林业科技档案利用的主要方式、特定要求,以及在林业科技档案利用过程中存在的知识产权保护问题,对如何在充分利用林业科技档案的同时保护好知识产权,进行了初步探讨。     

静松灵硬膜外腔注射对绵羊的镇静镇痛作用及其血液变化的研究

在１２只绵羊按０．３ｍｇ／ｋｇ剂量硬膜外腔注射静松灵,观测了镇静镇痛作用的效应,并对血液气体和酸碱平衡状态的变化进行了定量分析,结果表明静松灵硬膜外腔注射的镇静镇痛效果明显,显效时间短,持续时间达１２０ｍｉｎ,对体温,脉搏,呼吸与吸气量有显著作用（Ｐ〈０．０５）,对血液中氧分压,氧含量,氧饱和度,二氧化碳分压,剩余碱及碳酸氢盐的影响明显（Ｐ〈０．０５）,而对血液ｐＨ值和血红蛋白含量无显著影响。