全文获取类型
收费全文 | 846篇 |
免费 | 26篇 |
国内免费 | 94篇 |
专业分类
林业 | 1篇 |
农学 | 23篇 |
基础科学 | 1篇 |
18篇 | |
综合类 | 284篇 |
农作物 | 7篇 |
水产渔业 | 32篇 |
畜牧兽医 | 535篇 |
园艺 | 57篇 |
植物保护 | 8篇 |
出版年
2024年 | 2篇 |
2023年 | 5篇 |
2022年 | 17篇 |
2021年 | 23篇 |
2020年 | 23篇 |
2019年 | 24篇 |
2018年 | 16篇 |
2017年 | 19篇 |
2016年 | 34篇 |
2015年 | 26篇 |
2014年 | 36篇 |
2013年 | 37篇 |
2012年 | 60篇 |
2011年 | 59篇 |
2010年 | 38篇 |
2009年 | 57篇 |
2008年 | 43篇 |
2007年 | 65篇 |
2006年 | 45篇 |
2005年 | 33篇 |
2004年 | 34篇 |
2003年 | 22篇 |
2002年 | 23篇 |
2001年 | 22篇 |
2000年 | 23篇 |
1999年 | 27篇 |
1998年 | 12篇 |
1997年 | 14篇 |
1996年 | 14篇 |
1995年 | 11篇 |
1994年 | 19篇 |
1993年 | 14篇 |
1992年 | 13篇 |
1991年 | 13篇 |
1990年 | 12篇 |
1989年 | 6篇 |
1988年 | 10篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1983年 | 2篇 |
1980年 | 2篇 |
1976年 | 1篇 |
1956年 | 3篇 |
排序方式: 共有966条查询结果,搜索用时 15 毫秒
41.
【目的】真菌毒素可导致严重的食品安全问题。本试验旨在制备可用于检测伏马菌素B1的特异性单克隆抗体。【方法】制备伏马菌素B1人工抗原,杂交瘤细胞法筛选杂交瘤细胞株制备腹水型单克隆抗体,并对其特性进行鉴定。【结果】筛选得到杂交瘤细胞株F3,分泌的单克隆抗体亚类为IgG1,轻链类型为κ链;10%变性聚丙烯酰胺凝胶电泳显示单抗蛋白重链分子质量约为50 kD,轻链分子质量约为25 kD;间接酶联免疫吸附法测定杂交瘤细胞F3细胞培养上清和腹水效价分别为1:3 200和1:51 200;亲和力常数为1.60×10-8 mol•L-1;IC50值可达3.589 ng•mL-1;对其它真菌毒素不存在交叉反应;免疫转印法鉴定抗体具有高特异性。【结论】本试验制备FB1单克隆抗体具有较好亲和力和高特异性,具有良好的应用价值。 相似文献
42.
将猪流行性腹泻病毒LJB/03株S基因上编码中ps420(1 495~1 916 bp)基因片段插入乳酸乳球菌pNZ8112中,构建了重组表达载体pNZ8112-ps420,将其电转化入乳酸乳球菌Lactococcus lactis NZ9000,获得了表达猪流行性腹泻病毒S基因ps420的重组乳酸乳球菌,经乳链菌肽(... 相似文献
43.
44.
45.
[目的]制备氯霉素全抗原,探索氯霉素合成的方法,为动物免疫试验作准备。[方法]采用重氮化法合成氯霉素卵清蛋白全抗原,通过紫外法和红外光谱法测定合成的偶联效果。[结果]重氮化法可用于制备较高偶联率的氯霉素全抗原,用于免疫动物制备抗体。[结论]该研究为合成高效价的抗体奠定了基础。 相似文献
46.
鳖源嗜水气单胞菌M-96株表面抗原分析 总被引:1,自引:0,他引:1
用SDS-PAGE、免疫印迹技术分析嗜水气单胞菌M-96株表面抗原,表明该菌体表面抗原是由分子量为30KD、33KD、36K和39KD的蛋白质和脂多糖组成,其中部分对胃蛋白酶敏感。 相似文献
47.
48.
AIM: To study the effects of intrathymic inoculation of liver specific antigen (LSA) on hepatocyte apoptosis after liver allotransplantation. METHODS: Orthotopic liver transplantation was used in this study. Group Ⅰ: syngenic control (Wistar-to-Wistar); Group Ⅱ: acute rejection (SD-to-Wistar); Group Ⅲ: thymus inoculation of SD rat LSA day 7 before transplantation. The observation of general situation and survival time, hepatocyte apoptosis and LAT expression in liver transplants were used to analyze immune state of animals in different groups. RESULTS: The general situation of group Ⅰ was very well after transplantation. Recipients of groupⅡ lost body weight progressively and all died within day 9 to day 13 post transplantation. As for group Ⅲ, the general situation of recipients was remarkably better than that in group Ⅱ. The positive cells of apoptosis in group Ⅲ detected by TUNEL were not significantly different from that in group Ⅰ, but was significantly lower than that in group Ⅱ. LAT was detected at any time in group Ⅱ with peak expression at day 5 and day 7 post transplantation. In contrast, LAT was not detected in any other groups. CONCLUSION: Intrathymic inoculation of LSA protects hepatocytes from apoptosis after liver allotransplantation. 相似文献
49.
Atkinson RA Cook RW Reddacliff LA Rothwell J Broady KW Harper P Ellis JT 《Australian veterinary journal》2000,78(4):262-266
OBJECTIVE: To investigate the seroprevalence of Neospora caninum infection in a commercial dairy cattle herd, 15 months after detection of an abortion outbreak. PROCEDURE: Sera from the whole herd (n = 266) were examined for N caninum antibodies by indirect fluorescent antibody test (IFAT) and immunoblot analysis. Herd records were reviewed to collate serological results with abortion history, proximity to calving, and pedigree data. RESULTS: The seroprevalence of N caninum infection was 24% (63/266) for IFAT titre > or = 160, 29% (78/266) for immunoblot positive (+ve), and 31% (82/266) for IFAT > or = 160 and/or immunoblot +ve; 94% (59/63) of animals with IFAT > or = 160 were immunoblot +ve. The association between seropositivity (IFAT > or = 160 and/or immunoblot +ve) and history of abortion was highly significant (P < 0.001); the seroprevalence was 86% (18/21) in aborting cows, compared with 30% (50/164) in non-aborting animals. The abortion rate for seropositive cows was 26% (18/68) compared with 3% (3/117) for seronegative animals. IFAT titres of infected cows were higher within 2 months of calving than at other times (P < 0.001). The association between seropositivity in dams and daughters was highly significant (P = 0.009). CONCLUSIONS: The abortions were associated with N caninum infection and there was evidence of reactivation of latent infection close to calving and congenital transmission of infection. Immunodominant antigens identified by immunoblots may prove useful for improved diagnostic tests. 相似文献
50.
Brajesh C. Varshney N.M. Ponnanna Pranati A. Sarkar Pragna Rehman Jigar H. Shah 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):57-64
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to purified K99 antigen were produced by the hybridoma technique, and a specific clone, NEK99-5.6.12, was selected for propagation in tissue culture. The antibodies, thus obtained, were affinity-purified, characterized and coated onto Giemsa-stained Cowan-I strain of Staphylococcus aureus (S. aureus). The antibody-coated S. aureus were used in a co-agglutination test to detect K99+ E. coli isolated from feces of diarrheic calves. The specificity of the test was validated against reference monoclonal antibodies used in co-agglutination tests, as well as in ELISA. Specificity of the monoclonal antibodies was also tested against various Gram negative bacteria. The developed antibodies specifically detected purified K99 antigen in immunoblots, as well as K99+ E. coli in ELISA and co-agglutination tests. The co-agglutination test was specific and convenient for large-scale screening of K99+ E. coli isolates. 相似文献