全文获取类型
收费全文 | 2217篇 |
免费 | 85篇 |
国内免费 | 371篇 |
专业分类
林业 | 2篇 |
农学 | 36篇 |
基础科学 | 2篇 |
17篇 | |
综合类 | 563篇 |
农作物 | 1篇 |
水产渔业 | 174篇 |
畜牧兽医 | 1865篇 |
园艺 | 7篇 |
植物保护 | 6篇 |
出版年
2024年 | 6篇 |
2023年 | 20篇 |
2022年 | 50篇 |
2021年 | 66篇 |
2020年 | 67篇 |
2019年 | 84篇 |
2018年 | 39篇 |
2017年 | 49篇 |
2016年 | 82篇 |
2015年 | 85篇 |
2014年 | 124篇 |
2013年 | 92篇 |
2012年 | 176篇 |
2011年 | 192篇 |
2010年 | 118篇 |
2009年 | 137篇 |
2008年 | 129篇 |
2007年 | 200篇 |
2006年 | 154篇 |
2005年 | 130篇 |
2004年 | 113篇 |
2003年 | 77篇 |
2002年 | 74篇 |
2001年 | 75篇 |
2000年 | 75篇 |
1999年 | 38篇 |
1998年 | 32篇 |
1997年 | 39篇 |
1996年 | 17篇 |
1995年 | 25篇 |
1994年 | 26篇 |
1993年 | 14篇 |
1992年 | 18篇 |
1991年 | 10篇 |
1990年 | 10篇 |
1989年 | 10篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1979年 | 6篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1956年 | 2篇 |
排序方式: 共有2673条查询结果,搜索用时 31 毫秒
101.
在注射兔病毒性出血症 (RHD)疫苗后 ,30只兔随机分为 2组 ,试验组兔饲喂含 1 5 %黄白散的饲料。从接种到第 1 80天 ,每组取兔 8只 ,每隔 1 5d采血 1次 ,检测血凝抑制 (HI)抗体 ;在接种前 1天及接种后 5、 1 0、 1 7、 2 4、 31、 41、 5 1d ,每组取兔 1 0只 ,采血检测ANAE+淋巴细胞百分率 ;在 1 80、 2 4 0、 30 0d时 ,每组分别取兔 5只做攻毒保护试验。结果表明 :2组兔HI抗体峰值分别为 7 3log2和 9 8log2 ,差异极显著 (P <0 0 1 ) ;试验组疫苗保护期可延长 60d ;试验组ANAE+率从第 1 0天至 5 1天与对照组比较 ,差异极显著(P<0 0 1 )。所以黄白散可增强兔接种RHD疫苗后的特异性免疫功能 ,增强RHD疫苗的免疫效果。 相似文献
102.
103.
104.
105.
106.
牛病毒性腹泻/粘膜病O系弱毒冻干苗的试验生产和检验报告 总被引:5,自引:0,他引:5
1992 ̄1994年共试生产牛病毒性腹泻/粘膜病(BVD/MD)O系细胞培养弱毒冻干苗60万头份,通过实验室内安全检验、效力检验、无菌检验、水份测定、真空度检验、物理性状检验及在玉树、称多和泽库三个疫病发生严重的县试用,证明《BVD/MDO系细胞培养弱毒冻干苗制造与检验试行办法》可行,并为制定该苗的制造与检验规程提供了依据。 相似文献
107.
A Dam 《Acta veterinaria Scandinavica》1973,14(5):691-699
Experiments have been carried out with vaccination of pregnant mice against E. coli, followed by i.p. challenge of the offspring at one week of age.With a septicaemic strain the results were highly significant, and the method is therefore recommendable for testing of vaccines against such strains of E. coli.Results were less clear-cut with enteropathogenic strains of E. coli. However, with mortality rates of 40 to 45 % in baby mice born by non-vaccinated mothers and less than 15 % in baby mice born by vaccinated mothers, the difference in percentage mortality seems sufficient to warrant the use of the method also in the control of vaccines against enteropathogenic E. coli strains. 相似文献
108.
Poxviruses as vaccine vectors 总被引:4,自引:0,他引:4
Pastoret PP Vanderplasschen A 《Comparative immunology, microbiology and infectious diseases》2003,26(5-6):343-355
The discovery of Jenner in 1798 founded the science of immunology and eventually led to smallpox eradication from the earth in 1980 after a world-wide vaccination campaign with vaccinia virus (another poxvirus) and paradoxically, despite the eradication of smallpox, there has been an explosion of interest in vaccinia virus in the eighties. This interest has stemmed in part from the application of molecular genetics to clone and express foreign genes from recombinant vaccinia viruses. Vaccinia is also gaining renewed interest due to bioterrorism.
These recombinant viruses have multiple applications in research and vaccinology and led to the development of vectored vaccines, such as the recombinant vaccinia rabies vaccine used to eliminate rabies in Western Europe and, more recently, in the United States. Secondly, alternative poxvirus vectors, such as avipox viruses, were proved to be even safer and efficacious non-replicating vectors (suiciole vectors) when used in non-avian species. 相似文献
109.
Puaux AL Michel ML 《Comparative immunology, microbiology and infectious diseases》2003,26(5-6):357-372
Vaccine approaches against AIDS have focused on inducing cellular immune responses, since many studies revealed the role of T cell responses in the control of human immunodeficiency virus or simian immunodeficiency virus (SIV) infections. The experimental infection of rhesus macaques with SIV or chimeric SHIV is routinely used as a model for AIDS. In such models, DNA immunization is a tool to elicit specific T cell responses and to study their protective efficacy. DNA immunogenicity in primates depends on parameters such as level of antigen expression, choice of the antigen among SIV proteins, use of fusion proteins, route of immunization, and addition of adjuvants. Recent results suggest that priming with DNA and boosting with attenuated recombinant viral vectors, each expressing corresponding SIV antigens, leads to improved specific immunity and, in some cases, affords protection against pathogenic challenge. After preclinical evaluations, DNA has entered clinical trials for a therapeutic or prophylactic gene-based AIDS vaccine. 相似文献
110.
Terzić S Jemersić L Lojkić M Madić J Grom J Toplak I Sver L Valpotić I 《Veterinary research communications》2003,27(4):329-339
Ten pigs, aged 85 days, were vaccinated with a subunit vaccine containing 32 g of classical swine fever virus glycoprotein E2 (gp E2) (group 1), and a further 10 pigs were vaccinated with a C strain vaccine (104±0.15 TCID50/ml), produced by amplification in minipig kidney (MPK) cell culture (group 2). Nine non-vaccinated pigs served as a control group (group 3). Serum samples were collected before (day 0) and at 4, 10, 21 and 28 days after vaccination and were analysed by two commercially available enzyme immunoassays and by a neutralizing peroxidase-linked assay (NPLA). At the same times, peripheral blood was taken for determining the total leukocyte count and the body temperature was taken daily. Antibodies were not detected in serum samples collected before vaccination (day 0), and no side-effects that could be connected with vaccination were observed during the trial. Ten days after vaccination 6/10 pigs vaccinated with the subunit vaccine were seropositive. On days 21 and 28, the ratios of serologically positive to vaccinated pigs were 9/10 and 10/10, respectively. Four of the ten pigs that were vaccinated with the C strain vaccine were positive on day 21 and 9/10 on day 28. However, the results of the NPLA showed that only 4/10 pigs had an antibody titre >1:32 at the end of the trial in both the vaccinated groups, even though the subunit vaccine initiated an earlier and higher level of neutralizing antibodies than the vaccine produced from the C strain. Challenge was performed 28 days after vaccination on four randomly selected pigs from both vaccinated groups. The pigs survived the challenge without showing any clinical signs of classical swine fever (CSF), while two nonvaccinated control pigs died on the 10th and 12th days after infection. 相似文献