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Evaluation of dietary natural mineral materials as an antibiotic replacer on growth performance,non‐specific immune responses and disease resistance in rainbow trout,Oncorhynchus mykiss 下载免费PDF全文
Seonghun Won Mohammad Moniruzzaman Seunghan Lee Jeongwhui Hong Jung‐Keug Park Sangeun Kim Sungchul C Bai 《Aquaculture Research》2017,48(9):4735-4747
We evaluated the effects of some dietary natural mineral materials as an antibiotic replacer based on growth performance, non‐specific immune responses and disease resistance in juvenile and subadult rainbow trout, Oncorhynchus mykiss. First experiment, juvenile rainbow trout averaging 2.7 ± 0.02 g (mean ± SD) were fed one of the six experimental diets; a basal commercial diet as a control (CON), CON with oxytetracycline (OTC), with yellow loess (YL), with Macsumsuk® (MS), with Song‐Gang® stone (SG) and with barley stone (BS) at 0.4% of each diet. At the end of 8‐week feeding trial, weight gain (WG), specific growth rate (SGR), feed efficiency (FE) and protein efficiency ratio (PER) of fish fed YL diet were significantly higher than those of fish fed CON diet. Non‐specific immune responses such as superoxide dismutase (SOD), myeloperoxidase (MPO), lysozyme (LYS) activity and oxidative radical production of fish fed YL diet were higher than those of fish fed CON diet. At the end of 15 days of challenge test with Aeromonas salmonicida, average cumulative survival rate of fish fed YL diet was significantly higher than that of fish fed BS and CON diets. However, there were no significant differences among fish fed YL, SG and OTC diets. Second experiment, subadult rainbow trout averaging 261.5 ± 3.5 g (mean ± SD) were fed one of the four experimental diets for 22 weeks: CON, and CON with OTC, YL or SG at 0.4% of each diet. At the end of feeding, growth performance of fish fed SG and YL diets was significantly higher than that of fish fed CON diet. Non‐specific immune responses in terms of SOD, MPO, LYS and NBT of fish fed SG and YL diets were significantly higher than those of fish fed CON diet. However, there were no significant differences among the fish fed YL, SG and OTC diets. The results indicate that dietary yellow loess or Song‐gang® stone at 0.4% of diet could replace oxytetracycline in juvenile and subadult rainbow trout. 相似文献
73.
Ashkan Zargar Ali Taheri Mirghaed Seyed Saeed Mirzargar Melika Ghelichpour Morteza Yousefi Seyyed Morteza Hoseini 《Aquaculture Research》2020,51(10):4215-4224
In the present study, potential ameliorative effects of dietary ginger (GN) were investigated on antioxidant and immune responses of rainbow trout (Oncorhynchus mykiss) during oxytetracycline (OX) administration. As a 2 × 3 factorial design, the fish were orally treated with OX (a daily dose of 100 mg/kg) and GN (either 10 or 20 g/kg diet) for 10 days. Then, blood samples were taken from each treatment to monitor plasma lysozyme, complement (ACH50), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione‐S‐transferase (GST) activities, and reduced glutathione (GSH), malondialdehyde (MDA), total immunoglobulin (Ig) and globulin levels. OX treatment significantly decreased SOD (30%), GPx, (10%) and lysozyme (23%) activities, and GSH (19%) levels; however, it increased GST (16%) activity and MDA (28%) levels. Ten grams GN per kg levels significantly decreased SOD (35%), CAT (13%), GST (20%) and MDA (30%), but increased GSH (30%), lysozyme (48%) and globulin (16%). Twenty grams GN per kg diet significantly decreased SOD (26%) and MDA (17%), but increased lysozyme (31%) levels. Interaction effects of dietary GN and OX were observed on plasma MDA and GPx levels, as 10 g GN per kg diet prevented the OTC‐induced changes in these parameters. Moreover, 20 g GN per kg diet prevented the OX‐induced change in GPx activity and mitigated the MDA elevation by 20%. It is concluded that GN administration at 10 g/kg diet is beneficial in mitigating oxidative stress and immunosuppression of rainbow trout during OX administration. 相似文献
74.
The purpose of this research was to evaluate the antibiotic resistance in two water reclamation systems developed from space missions. The first system is a small-scale water reclamation system operated at Johnson Space Center designed to reclaim wastewater during long-term space missions. The second system was a scaled-down version of the Johnson Space Center system operated at Texas Tech University. Antibiotic resistance patterns to 10 antibiotics were investigated before and after controlled doses of amoxicillin were added to the water reclamation systems. The results of this study indicate that bacteria in all systems were resistant to many antibiotics including beta-lactam antibiotics and a beta-lactam, beta-lactamase inhibitor combination, amoxicillin with clavulanic acid. 相似文献
75.
以杭州乳房炎诊断法(HMT)、细菌学检验和临床检查结果为依据,综合判断PC-805和TA-125两种抗菌素油剂对干奶期乳房炎的防治效果。66头受试奶牛随机分成两组,第一组35头136个乳区于停奶当天经由乳头管注入20ml PC-805;第二组31头121个乳区以同样方法注入20ml TA-125。第一组牛在干奶前有72个乳区为HMT强阳性(++、+++),至产后降为11个,转阴率为84.7%;第二组牛在干奶前HMT强阳性乳区为66个,产后降至6个,转阴率为90.9%。第一、二组HMT强阳性乳区的细菌转阴率分别为57.4%和68.4%,除去新感染后则分别为70.5%和75.4%。这两种制剂对链球菌感染的效果较好,而对葡萄球菌的作用较差,第一组牛在千奶期间的临床发病率为2.9%,第二组为1.7%。尽管TA-125的疗效较好,但统计结果表明这两种制剂对HMT和细菌转阴率以及临床发病率方面均无显著差异(P>0.05)。 相似文献
76.
多烯大环类抗生素的生物合成酶属于典型的Ⅰ类聚酮合酶(PKS),这些生物大分子有着非常相似的化学结构和模块合成的形式.在本研究中,每个多烯聚酮合酶的单一结构域被作为系统进化分析的对象.我们发现,各类结构域在进化上出现了不同方向的倾向,其中,KS域在进化树中归成三大类,分别对应多烯结构的三大块区域;相对于KS域和ACP域表... 相似文献
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79.
海洋弗氏链霉菌HNM0089抗真菌活性成分研究 总被引:1,自引:0,他引:1
为了从海洋微生物中寻找农药先导化合物,本研究通过16S rRNA基因序列分析对具有抗真菌活性的海洋放线菌HNM0089的种属进行鉴定,并对其抗真菌的活性成分进行研究。结果表明:海洋放线菌HNM0089被鉴定为弗氏链霉菌(Streptomyces fradiae)。利用高分辨质谱、一维和二维核磁波谱技术,将其主要活性成分鉴定为星形孢菌素(Staurosporine)。该化合物对芒果炭疽病菌、水稻稻瘟病菌、香蕉枯萎病菌、薯蓣炭疽病菌和橡胶炭疽病菌均有抑制活性,具有开发成农用抗菌素的潜力。 相似文献
80.
海洋青霉菌抗真菌活性物质高产菌株的诱变选育 总被引:4,自引:0,他引:4
采用紫外线(UV)、激光(Laser)、亚硝基胍(NTG)复合诱变HN89青霉菌后,再用HN89菌株的次生代谢产物(HN89A)对复合诱变菌株进行了自我抗性的选育.结果表明,与出发菌相比,菌株经诱变后相对效价显提高.自我抗性诱变结果表明:在67株经复合诱变的抗性菌株中,其抗真菌活性物质相对效价高于出发菌株的正变株有60株,正变率达到90%。其中相对效价提高50%以上的菌株为2l株,占所筛选菌株总数的31%;效价提高100%以上的菌株为9株,占13%,其中突变株HN89-68活性物质的相对效价可提高到286%。活性物质效价提高幅度与其自身产量提高幅度存在一定的正相关。诱变高产菌株遗传稳定性好. 相似文献