Assessment of chosen semen characteristics of two colour morphs of the Arctic fox Alopex lagopus L.

The aim of the study was to evaluate semen quality in the two most popular colour morphs of the Arctic fox Alopex lagopus L., blue and white, based on ejaculate parameters, acrosin activity and analysis of sperm morphology. The research material consisted of ejaculates collected once by manual stimulation from 20 one‐year‐old male Arctic foxes (10 individuals of the blue morph and 10 of the white morph). Ejaculates were evaluated in terms of volume, sperm concentration, total number of spermatozoa and the percentage of spermatozoa with major and minor defects. The study revealed that male blue Arctic foxes produce ejaculates with much higher concentration (148.75 × 10⁶/ml) and total number of spermatozoa (98.16 × 10⁶) compared to white Arctic foxes (42.88 × 10⁶/ml and 35.2 × 10⁶ respectively). The level of acrosin activity from white foxes seemed to be higher compared to blue foxes but the difference was not statistically confirmed. Semen from Arctic foxes is characterized by high inter‐individual variability in sperm morphology. The frequency of morphological changes in sperm from Arctic foxes does not significantly depend on ejaculate volume, sperm concentration or the total number of spermatozoa in the ejaculate, but is associated with acrosin activity.     

Variation in protein concentrations in acute and chronic ascites in Dirofilaria immitis-infected dogs

Microfilaremic dogs, developing ascites acutely following a reaction to diethylcarbamazine therapy, had similar protein concentrations in their ascitic fluid and plasma. In contrast, in dogs chronically infected with Dirofilaria immitis, the protein concentrations of ascitic fluid were found to be significantly lower than plasma protein concentrations. The acute development of ascites in such dogs is associated with high ascitic protein levels.     

HPLC法测定猪体内双氯芬酸的血药浓度

建立猪血浆中双氯芬酸含量的HPLC测定法。猪血浆中双氯芬酸采用正己烷-异丙醇混合溶液(90∶10,V/V)提取,HPLC紫外检测器检测。色谱柱为C18柱,流动相为甲醇-15g/L乙酸水溶液(80∶20,V/V),测定波长为276nm。外标法定量。结果表明,血浆中杂质不干扰双氯芬酸的测定,双氯芬酸血药浓度在0.025μg/mL~20.0μg/mL范围内线性关系良好(R2=1),最低定量限为0.05μg/mL,高、中、低空白血浆添加样品浓度的日内变异系数和日间变异系数分别小于4%和6%,回收率为85%~97%。结果表明,该方法适用于猪血浆中双氯芬酸含量的测定,可用于含双氯芬酸钠的制剂在猪体内的药物代谢动力学和生物利用度研究。     

奶牛日粮中添加富硒酵母对乳硒和血清硒含量的影响

通过日粮添加富硒酵母研究有机硒对泌乳奶牛乳硒含量和血清硒含量的影响。试验选择24头胎次相同,泌乳日龄、产奶量和体重相近的荷斯坦奶牛,随机分为2组,对照组和处理组均饲喂相同的TMR基础日粮,TMR基础日粮硒含量为0.33 mg/kg,处理组另补充富硒酵母,源于富硒酵母的硒为0.78 mg/kg DM,整个试验期56 d。试验结果表明,添加富硒酵母后对奶牛干物质采食量、产奶量、乳常规和乳常规产量没有显著影响,但显著提高了血清和牛奶中硒含量,分别为123.1和97.7 μg/L,与对照组相比,血清硒含量和牛奶硒含量分别提高了60.5%和194.3%。同时,在整个试验期内,血清硒含量呈上升趋势,而牛奶硒很快达到了稳定状态。     

Temporal dynamics of nutrient and carbohydrate distribution during crop cycles of Rosa spp. ‘Kardinal’ in response to light availability

Rose plants that are flush harvested exhibit episodic growth patterns. During these crop cycles little biomass accumulation occurs immediately following harvest; and as new shoots emerge a period of rapid shoot growth and biomass accumulation occurs. The temporal changes in whole-plant nutrient and carbohydrate distribution during these crop cycles and the role of storage in new shoot growth are not well documented. The objective of this project was to quantify N, P, K, and total non-structural carbohydrates (TNC) distribution in roots, base stems, base leaves, and new shoots during crop cycles in response to light availability. Plants were grown in solution culture under high or low light (mean daily light integral 45.3 or 13.1 mol m⁻² d⁻¹, respectively) during 30–35 day crop cycles. Every five days destructive sampling was used to determine biomass and N, P, K, and TNC concentration of rose plant compartments. N and TNC accumulated in base plant compartments during the first ten days of the crop cycles. N, P, K, and TNC in base plant compartments declined during days 10–25 during a crop cycle concurrent with the rapid growth of flower shoots. N, P, and K storage in base plant parts represents 27, 22, and 24% of the potential N, P, and K required by flower shoots under high light; and 19, 21, and 22% of requirements under low light. TNC storage in base plant parts represents 4–10% of the final biomass of flower shoots. Mobilization of N, P, K, and TNC stored from base plant parts appears to be important during the stage of rapid flower shoot growth when absorption by roots or photosynthesis by shoots was insufficient to meet flower shoot demands. Plant carbohydrate status was improved under high light conditions; storage of N and TNC declined under low light.     

Plant water stress and K starvation reduce absorption of foliar applied K by olive leaves

Three-month-old mist-rooted ‘Picual’ olive cuttings were transplanted into 2-l plastics pots containing perlite as substrate and fertigated with a complete nutrient solution containing 0.05, 0.1 or 2.5 mM KCl depending on the experiment. In the first experiment, plants were sprayed with RbCl (Rb⁺ is a K⁺ analog) at a rate of 4% at 63 days after transplanting (DAT). Foliar Rb⁺ uptake through leaves increased with K⁺ concentration in the nutrient solution, indicating that foliar Rb⁺ uptake was lower when plants were K⁺ deficient than when they were adequate. On the contrary, it was observed that translocation of Rb⁺ from leaves to other organs of the plant was higher under K⁺ deficiency conditions. In the second and third experiments, when differences appeared on shoot length due to K⁺ nutritional status (0.05 or 2.5 mM KCl) at 63 DAT, a group of plants were subjected to water stress during 7 weeks. On the other hand, another group of plants (control plants) did not receive any water stress treatment during the experiments. After this period of 7 weeks, all plants were sprayed with RbCl at 4%. Leaf K⁺ concentration diminished in water-stressed plants independently of plants nutritional status. Foliar Rb⁺ uptake through leaves was restricted by water stress either in plants with low K⁺ (0.05 mM KCl) or plants with high K⁺ (2.5 mM KCl). Translocation of Rb⁺ from leaves was greater under water stress conditions in both K⁺ nutritional statuses. In conclusion, the results obtained could explain the irregular response in olive trees to foliar K⁺ sprays, particularly, when they grow in rainfed orchards.     

Effects of interlighting on yield and external fruit quality in year-round cultivated cucumber

The effects of interlighting and of the proportion of interlight on the yield and fruit quality of year-round cultivated cucumber (Cucumis sativus L. cv. Cumuli) were investigated for this study. Artificial lighting was provided by high pressure sodium (HPS) lamps and the lighting regimes included top lighting (TL), top + interlighting 24% (T + IL24) and top + interlighting 48% (T + IL48). In TL, all of the lamps were mounted above the canopy. In T + IL24 and T + IL48, top lamps covered 76 and 52% of the lighting, respectively, while 24 and 48% of the lighting came from interlighting lamps which were mounted vertically 1.3 m above the ground between the single plant rows. The outdoor daily light integral (DLI) varied greatly during the cultivation periods; the mean values were 36.8, 5.3 and 19.9 mol m⁻² day⁻¹ for the summer, autumn–winter and spring stands, respectively. Lighting regime affected both yield and external fruit quality. Interlighting increased first class yield and decreased unmarketable yield, both in weight and number. The increase in the annual first class yield in weight was 15% in the two T + IL regimes. Interlighting improved energy use efficiency in lighting, being for the whole year 120, 130 and 127 g total yield kW h⁻¹ in TL, T + IL24 and T + IL48, respectively. Interlighting increased the fruit skin chlorophyll concentration in all seasons, but had only a minor effect on the fruit dry matter concentration. The mean total chlorophyll concentration in fruit skin was 70.8, 76.7 and 82.2 μg cm⁻² in TL, T + IL24 and T + IL48, respectively. In addition, interlighting extended the post-harvest shelf life of cucumber fruits in spring. Besides interlighting per se, also the higher proportion of interlight tended to further improve the fruit quality. For example, the fruit skin chlorophyll concentration increased along with the higher proportion of interlighting. In general, the effects of lighting regime were more prominent in lower natural light conditions in winter and spring. It is concluded that interlighting is a recommendable lighting method in cucumber cultivation, especially in lower natural light conditions.     

Root restriction-induced limitation to photosynthesis in tomato (Lycopersicon esculentum Mill.) leaves

Root restriction often depresses photosynthetic capacity and the mechanism for this reduction, however, remains unclear. To identify the mechanism by which root restriction affects the photosynthetic characteristics, tomato (Lycopersicon esculentum Mill.) seedlings were subjected to root restriction stress with or without supplemental aeration to the nutrient solution. With the development of the root restriction stress, CO₂ assimilation rate was decreased only in confined plants without supplemental aeration. There were also significant decreases in leaf water potential, stomatal conductance (g_s), intercellular CO₂ concentration (C_i), and increases in the stomatal limitation (l) and the xylem sap ABA concentration. Meanwhile, the maximum carboxylation rate of Rubisco (V_cmax) and the capacity for ribulose-1,5-bisphosphate regeneration (J_max) also decreased, followed by substantial reductions in the quantum yield of PSII electron transport (Φ_PSII). Additionally, root restriction resulted in accumulation of carbohydrates in various plant tissues irrespective of aeration conditions. It is likely that root restriction-induced depression of photosynthesis was mimicked by water stress.