The effect of C/N ratio on bacterial community and water quality in a mussel‐fish integrated system

A 30‐day experiment was conducted to evaluate the effect of C/N ratio on water quality and bacterial community in an integrated system comprising one molluscan species (pearl mussel Hyriopsis cumingii) and two fish species (gibel carp Carassius gibelio and silver carp Hypophthalmichthys molitrix) at five C/N ratios (6, 8, 10, 12 and 14). The mussel and fishes were reared in the experimental tanks (400 L), but gibel carp received formulated feed. Water quality in the experimental tanks was analysed on day 0, 10, 20 and 30, and bacterial community in the water column and sediment was analysed on day 30. Total nitrogen, total phosphorus and total organic carbon accumulated in the tanks over time. Ammonia and nitrite decreased with the increase in C/N ratio. Bacterial community in the water column and sediment changed at the phylum and genus levels with the increase in C/N ratio, and the critical C/N ratio causing a functional shift of bacterial community occurred at 10 in water column and 12 in sediment. The increase in C/N ratio benefited the growth of both potential probiotics and pathogenic bacteria. The high C/N ratio enhanced the bacterial functions of chemoheterotrophy and hydrocarbon degradation, but depressed the functions of nitrification and denitrification in the water column and sediment respectively. This study reveals that the C/N ratio can be used as a tool to manipulate the bacterial community and water quality in the mussel‐fish integrated system.     

Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method

In chickens, primordial germ cells (PGCs) are effective targets for advanced genome editing, including gene knock-in. Although a long-term culture system has been established for chicken PGCs, it is necessary to select a gene-editing tool that is efficient and precise for editing the PGC genome while maintaining its ability to contribute to the reproductive system. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) and CRISPR-mediated precise integration into the target chromosome (CRIS-PITCh) methods are superior as the donor vector is easier to construct, has high genome editing efficiency, and does not select target cells, compared to the homologous recombination method, which has been conventionally used to generate knock-in chickens. In this study, we engineered knock-in chicken PGCs by integrating a fluorescent protein gene cassette as a fusion protein into the chicken vasa homolog (CVH) locus of chicken PGCs using the CRIS-PITCh method. The knock-in PGCs expressed the fluorescent protein in vitro and in vivo, facilitating the tracking of PGCs. Furthermore, we characterized the efficiency of engineering double knock-in cell lines. Knock-in cell clones were obtained by limiting dilution, and the efficiency of engineering double knock-in cell lines was confirmed by genotyping. We found that 82% of the analyzed clones were successfully knocked-in into both alleles. We suggest that the production of model chicken from the knock-in PGCs can contribute to various studies, such as the elucidation of the fate of germ cells and sex determination in chicken.     

中国国民收入与茶叶内销量的实证研究

通过Eviews31软件就1990年到2008年我国国民收入与茶叶内销售量关系进行分析，得出我国国民收入与茶叶内销量存在协整关系，同时运用格兰杰检验得出我国国民收入提高是我国茶叶内销量增加的成因，认为通过预测我国国民收入水平可推算出我国茶叶的内销量，为茶叶产销决策提供依据。     

农业科研院所科研开发模式研究

阐述了农业科研院所在科研开发过程中的发展现状,指出了其存在的主要问题:精力与经费不足,科研开发两头难以兼顾;产权制度缺陷可能导致农业科研院所背上沉重的包袱;缺乏与企业合作,低水平重复研究现象比较普遍。提出了农业科研院所科研开发模式的建议:优先考虑技术成果直接转让模式,其次推崇合作开发—技术入股模式,最后要慎用创办企业—科研生产销售一体化模式。     

基于SALUS与WebGIS整合的作物生长模拟

SALUS是较为著名的土地可持续使用系统模型，实现了一种基于WebGIS整合SALUS的作物生长仿真系统。详细阐述了数据库和相关功能模块的整合方法，改善了SALUS模型在空间数据处理与仿真结果视觉化的能力。可以让使用者清晰的掌握土壤、天气、灌溉量、施肥量等多种因素对农作物产量的影响，为农作物实施相关的增收管理措施提供了一定的科学依据。并提出了相应的整合方案为实际构建基于WebGIS的作物生长仿真系统提供了一定的技术支撑。     

发展小城镇 推进城乡一体化进程

根据福建省南靖县城镇化发展现状与存在问题,分析了城镇化发展战略的可能与需求,提出了城乡一体化进程的发展路径。     

基于SOA的整车企业技术数据集成研究

针对整车企业信息化程度越来越高，但是信息孤岛现象也越来越严重的问题，提出了基于SOA（Service Oriented Architecture)的整车企业跨系统技术数据查询和实时报表的系统设计和实施框架，通过技术方案、用例、类图等详细分析了系统的构架和实施策略.案例研究表明，该系统具有很强的可操作性.     

长三角经济发展战略定位中探索图书馆发展的新模式

21世纪长三角经济发展必须要有一个正确的战略定位,区域内图书馆以此为指导,统一理念,共同探索图书馆发展的新模式。基于长三角经济发展现状的分析,提出了21世纪长三角经济发展的战略定位对图书馆建设与发展的影响,并阐述了构建特色的馆藏建设是图书馆发展的基础。     

城乡一体化视角中贵州农村劳动力素质研究

推进城乡一体化,建设社会主义新农村,需要具有高素质的新型农民。目前贵州省农民整体素质相对较低,与全面建设小康社会不相适应。因而在城乡一体化过程中,要发挥农民在新农村建设中的主体作用,就应把提高农民素质、培育新型农民作为一项战略任务,加大农村教育培训的力度,采用多种形式,实施培训工程,培养有文化、懂技术、会经营的新型农民。     

阜阳剪纸艺术在新媒体语境下的传播研究

纵观阜阳剪纸的渊源与现状,认为阜阳剪纸是一门古老的民间艺术,是安徽省国家级非物质文化遗产,在皖北地区颇具影响。然而阜阳剪纸"地域相承、亲缘相承、师徒相承"的传播方式,极大地限制了它的发展潜力。研究着重探讨了阜阳剪纸资源的整合方法与整合平台的建立,认为新媒体时代的到来为阜阳剪纸艺术的传播敞开了从区域到全国乃至世界的大门,因此,利用多种新媒体技术途径,对阜阳剪纸艺术进行资源整合,建立多媒体传播系统,使阜阳剪纸艺术在传承中得到更广泛的传播,显得尤为重要。