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41.
邹庆云 《湖南农业大学学报(自然科学版)》2002,29(2)
提出统计学中的组距分组资料方差和标准差计算公式误差问题 ,由方差的加法定理和均匀分布方差给出分组资料方差计算和标准差计算的修正公式具体形式 ,并就均匀分布数列和正态分布数列组内方差平均数进行了讨论 . 相似文献
42.
Breeding of Raphanus sativus‐Brassica rapa monosomic chromosome addition lines (MALs, 2n = 19) was carried out by backcrossing the synthesized amphidiploid line, Raphanobrassica (R. sativus×B. rapa, 2n = 38, RRAA, line RA89) with R. sativus cv. ‘Shogoin’ (2n = 18, RR). In the first cross of Raphanobrassica× radish, four sesquidiploidal BC1 plants (2n = 28, RRA, RA89‐36‐1, RA89‐31‐1, RA89‐31‐2, RA89‐31‐3) were successfully developed. In these plants, the chromosome configurations of 9II + 10I and 10II + 8I were observed frequently at first metaphase (MI) of meiosis in pollen mother cells (PMCs). The RA 89‐36‐1 plant produced many seeds in the reciprocal backcrosses with radish. About 50% of the BC2 plants obtained from the cross of RA89‐36‐1 plant × radish were 2n = 19 plants, followed by 2n = 18 plants (24%) and 2n = 20 plants (19%). In the reciprocal cross, 2n = 19 plants were also developed at the rate of 40%. From analysis of specific morphological traits, 2n = 19 plants were classified into eight types (a‐h). When 25 selected primers were used in polyacrylamide gel electrophoresis, random amplified polymorphic DNA (RAPD) markers derived from B. rapa for each type of MAL were detected in numbers between three for e‐type and 16 for b‐type. RAPD markers specific for each type alone were from one (OPE 05‐344) for h‐type to nine for b‐type. In the g‐type, no marker specific to this type alone was observed. However, 19 bands were common between at least two types. These MAL plants exhibited predominantly the chromosome configuration of 9II + 1I at MI of PMCs, pollen and seed fertility being the same level as the radish cv. ‘Shogoin’. From the morphological traits and DNA markers, eight different MAL types among 10 expected were identified. 相似文献
43.
Monosomic addition lines of Beta corolliflora in sugar beet: plant morphology and leaf spot resistance 总被引:2,自引:0,他引:2
Monosomic addition lines in Beta vulgaris from Beta corolliflora were described morphologically and characterized for disease resistance. Monosomic addition plants (2n= 19) were selected among segregating offspring by a squash dot technique in combination with B. corolliflora‐specific probes. Plants carrying an added chromosome were characterized by leaf shape, plant size and plant vigour. In this way, most addition lines could be distinguished from diploid beets, however, to identify those plants unequivocally, molecular marker analysis was also necessary. Transmission frequencies of each addition line were determined to be in the range 13.9% (Cor‐4) to 60% (Cor‐9). High transmission rate of addition line Cor‐9 was assumed to be due to apomictic propagation because transmission rate after selfing cannot exceed 50%. Cercospora leaf spot resistance tests were performed on 167 monosomic plants from seven different addition lines, two fragment addition lines and 89 diploid controls. No line exhibited complete resistance, but the monosomic additions Cor‐3 and Cor‐4 showed significantly lower infection rates than their diploid sibling plants. The identification of monosomic addition lines with apomictic and disease resistance characters offers the possibility of transferring those genes to sugar beet. 相似文献
44.
A previous RFLP analysis showed that the Aegilops ventricosa chromosome 6MV which compensates for the absence of 6D in 6MV (6D) wheat substitution lines was a 2/6 translocated chromosome, either 2S–6S.6L or 2S–6L.6S. The distal part of its long arm consists of a translocated segment belonging to homoeologous group 2. Chromosome 6MV carries a gene(s) for resistance to cereal cyst nematode. In order to define the part of 6MV (2S or 6S or 6L) involved in this resistance, addition lines with a 6MV deleted either for its short arm or for the distal part of its long arm were evaluated. It was shown that the gene(s) is carried by the group 2 translocated segment. The hypothesis that the gene(s) could be allelic to Cre2, another gene conferring resistance to the nematode introduced into the wheat complement from Ae. ventricosa is discussed. 相似文献
45.
Among the wheatgrasses that are possible sources of genetic resistance for wheat to barley yellow-dwarf-virus disease (BYD) are those that have been commonly subsumed under the name Agropyron elongatum (Host) P. Beauv. Two of these wheatgrass species are the diploid Lophopymm elongatum (Host) Á. Löve (2n = 2x = 14) and the decaploid L. ponticum (Podp.) Á. Löve (2n = 10x = 70). These two species, the addition and substitution lines of L. elongatum chromosomes in hexaploid wheat (Triticum aestivum L.), and derivatives of hybrids between hexaploid wheat and L. ponticum, were screened for resistance to BYD, as defined by visual symptoms in field-grown plants. The two species, an amphiploid derived from L. elongatumבChinese Spring’ wheat, and the derivatives involving L. ponticum chromosomes were all highly resistant. The substitution and addition lines of L. elongatum chromosomes in ‘Chinese Spring’ revealed that the genetic control of resistance in L. elongatum must be complex, with more than one critical locus involved. Chromosomes 2E and 5E are involved and there are lesser contributions to resistance from the remaining wheatgrass chromosomes. One highly resistant derivative was determined to have only three pairs of L. ponticum chromosomes. It has a wheat-like morphology and shows promise for further characterization. 相似文献
46.
Two disomic barley chromosome addition lines and five translocated chromosome addition lines of common wheat cultivar Shinchunaga
were isolated. They were derived from a hybrid plant between Shinchunaga and cultivated barley Nyugoruden (New Golden) by
backcrossing with wheat and self pollination. Barley chromosomes added to chromosome arms involved in the translocated chromosomes
were identified by C-banding method and by crossing these lines with Chinese Spring/Betzes addition lines. Two disomic addition
lines were identified to have chromosome 6 and 7 of barley, respectively. Two of the five translocated chromosome addition
lines were clarified to have same chromosome constitution, 42 wheat chromosomes and a pair of translocated chromosomes constituted
with a long arm of chromosome 5B of wheat and a short arm of chromosome 7 of barley. The other three lines could not be identified
due to chromosome rearrangement. Performances of these seven lines on agronomic characters were examined. Addition of barley
chromosome 7 induced early heading, and chromosome 6 showed lated heading. Almost all of the lines except that of chromosome
6 showed short culm length and all showed reduced number of tillers, spikelets and grains per ear, and low seed fertility.
These lines would be useful for genetic analyses in wheat and barley and for induction of useful genes of barley into wheat.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
47.
Development of B-Genome Chromosome Addition Lines of B. napus Using Different Interspecific Brassica Hybrids 总被引:3,自引:0,他引:3
By interspecific hybridization within the genus Brassica, trigenomic haploids were produced and back-crossed four times with B. napus, variety ‘Andor’. From this material, monosomic B-genome chromosome addition lines were selected with the extra chromosome derived from three different B-genome sources, i.e., B. nigra (BB), B. carinata (BBCC), and B. juncea (AABB). After selfing and/or microspore culture, disomic addition lines were obtained. Meiotic behavior was studied of the trigenomic hybrids, the pentaploid BC1 plants, and the monosomic addition lines. The addition lines were shown to possess cytological stability and good fertility. 相似文献
48.
Zhi-Shan Lin Zhi-Fu Cui Xiang-Yan Zeng You-Zhi Ma Zeng-Yan Zhang Toshiki Nakamura Goro Ishikawa Kazuhiro Nakamura Hisashi Yoshida Zhi-Yong Xin 《Euphytica》2007,158(1-2):109-118
Chromosome compositions of seven lines, derived from hybrids between a wheat cultivar and the wheat-Thinopyrum intermedium addition line Z6, with barley yellow dwarf virus (BYDV) resistance, were determined by genomic in situ hybridization, cytogenetic and SSR assays. The results showed that
line N522 was a disomic addition line, lines N420 and N439 were 2Ai-2(2B) chromosome substitution lines, lines N431 and N452
were 2Ai-2(2D) chromosome substitution lines, line N523 was a 2Ai-2S(2D) ditelosomic substitution line, and line N530 was
a double ditelosomic line with the mitotic chromosome number of 2n = 40 + 4t. One pair of telosomes in line N530 lacked several proximal SSR markers of chromosome 2AS, but possessed certain
terminal markers, which were consistent with an acrocentric structure, and the other pair of chromosome arms were presumably
2Ai-2S telosomes with BYDV-resistance. These wheat-Th. intermedium lines provide useful genetic resources for developing alien chromosome translocation lines. 相似文献
49.
An interspecific hybrid involving Pennisetum americanum (2 n = 14) and a diploid cytotype of P. orientale (2 n = 18) was backcrossed to P. americanum using the hybrid (2 n = 16, 7‘A’+ 9 ‘O’) as the female parent. Pollen mother cells of 13 BC1 plants contained a complement of 14‘A’+ 9 ‘O’ chromosomes. Five BC2 plants obtained through further backcrossing to P. americanum had 21‘A’+ 9 ‘O’ chromosomes revealing another addition of the P. americanum genome. The role of such recurrent additions of parental genomes in the evolution of polyploid species has been discussed. 相似文献
50.
Homoeological relationships between the f chromosome of Brassica rapa and the e chromosome of Brassica oleracea 总被引:1,自引:0,他引:1
Eight plants of the putative double monosomic addition line (DMAL, 2n= 20) were developed by crossing a monosomic chromosome addition line of radish [f(A)‐type monosomic addition line (MAL) (2n= 19)] carrying the f chromosome of Brassica rapa (2n= 20, AA) with another [e(C)‐type MAL (2n= 19)] having the echromosome of Brassica oleracea (2n= 18, CC). The homoeological relationships between the two alien chromosomes were investigated by morphological, cytogenetic and random amplified polymorphic DNA (RAPD) analysis. Seventeen morphological traits that were not present in the radish cv. ‘Shogoin’ were observed in both MALs and these traits were substantially exhibited in DMAL plants. At the first metaphase of pollen mother cells (PMCs), the two parental MALs showed a chromosome configuration of 9II +1I, demonstrating impossibility of recombination between the R and the added chromosomes. The DMALs formed 10II in approximately 73% of PMCs, with one bivalent showing loose pairing between two chromosomes differing in size. In an attempt to identify the two MALs by RAPD‐specific markers using 26 selected random primers, 13 and 20 bands were specific for the f(A)‐type and the e(C)‐type MALs, respectively; 12 bands were common to both MALs (26.7%). In conclusion, the f chromosome of B. rapa is homoeologous to the e chromosome of B. oleracea. The genetic domain (genes) for 17 morphological traits are linked to each homoeologous chromosome bearing 27% of the corresponding RAPD markers. 相似文献