瓜类嫁接栽培技术探讨

从6个瓜类砧木品种中，选出了黄瓜适宜砧木为黑籽南瓜，苦瓜适宜砧木为黑籽南瓜和本地肉丝瓜，西瓜适宜砧木为超丰F1和欣京砧一号。瓜类嫁接方法以顶插接法和劈接法为宜，嫁接时期在3月初为宜。重病地黄瓜嫁接苗比自根苗枯萎病发病率降低95．8％，产量提高34．9％；嫁接苦瓜发病率降低83．7％，增产16．9％；嫁接西瓜发病率降低88．9％，增产13．7％。     

鸡传染性腔上囊病病毒VP2基因在昆虫细胞中的表达

为了深入研究鸡传染性腔上囊病病毒（IBDV）VP2基因的结构和功能,利用Bac-to-Bac系统研制出含有VP2基因的重组杆状病毒rBac-VP2,将rBac-VP2感染Sf9细胞,并用抗IBDV VP2特异性单克隆抗体经间接免疫荧光试验检测,证实感染重组病毒Sf9细胞能高效表达IBDV VP2基因产物;Western-blotting分析结果表明,VP2基因表达产物的分子质量约为40 ku.     

MAGNETIC RESONANCE IMAGING FINDINGS IN SPINAL CORD INFARCTION IN THREE SMALL BREED DOGS

Fibrocartilaginous embolization (FCE) of the spinal cord is a common disease in large breed dogs. There are only a few reports about this entity in small breed dogs and it has never been reported in chondrodystrophic breed. For definitive diagnosis histopathologic examination is necessary. Magnetic resonance imaging (MRI) as a potential diagnostic tool for intravitam diagnosis of FCE has been mentioned before, but results have not been reported so far. This report describes the neurological findings and MRI results in three small breed dogs, including a Pekingese dog, with FCE of the spinal cord. The disease was suspected in two animals based upon clinical and MRI-appearance and confirmed in the third by histopathological examination. In all three cases, similar focal intramedullary lesions, consisting of hyperintensive signals on T2-weighted images, were detected. Based on these findings, high-field MRI may be used as an antemortem tool for the diagnosis of FCE. It is also shown that FCE can occur in chondrodystrophic dogs.     

鹅副粘病毒病的研究进展

鹅副粘病毒病是近年来引起各种年龄鹅发病并致死的一种烈性传染病。目前，本病在我国许多省市流行，具有很高的发病率和死亡率。本文从临床症状、病理变化、病毒的组织嗜性、生物学特性和分子生物学性状等方面对鹅副粘病毒病作一综述。     

类鼻疽的研究进展

本文就类鼻疽(melioidosis)的流行病学,诊断方法,免疫机理,发病机理及治疗方法 进行了综述。为重视该病的研究提出了建议。     

陕西线辣椒病毒病病原检测简报

在陕西省线辣椒主产区多点随机取样,采用酶联免疫吸附法对陕西线辣椒病毒病毒原进 行了鉴定。结果表明,陕西线辣椒病毒病毒原有BBWV、PMMV、CMV、ToMV、TMV、PVY和 CVMV。优势毒原是BBWV、PMMV和CMV。     

中国香瓜与菜瓜地方品种资源白粉病抗性评价

春秋2个季节在温室利用风媒接种方法对搜集的102份香瓜和14份菜瓜种质资源进行了白粉病抗性评价,结果鉴定出7份香瓜和1份菜瓜高抗白粉病,其病情指数为零,1份菜瓜抗病,其病情指数0.9,表明我国香瓜和菜瓜地方品种资源中蕴涵着潜在的改良甜瓜白粉病抗性的基因资源。     

Role of p38MAPK in production of pro-inflammatory cytokines in Kupffer cells from severe acute pancreatitis rats

AIM:To investigate the role of p38 mitogen-activated protein kinase (p38MAPK) signaling pathway in the Kupffer cells (KCs) production of pro-inflammatory cytokines, tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β), in severe acute pancreatitis (SAP) rats.METHODS:Sprague-Dwaley rats were randomized into three groups:①sham operation rats, ②SAP rats, ③SAP rats given the p38 MAPK inhibitor CNI-1493(10 mg/kg, iv). The SAP model was induced by the bili-pancreatic duct infusion with 5% sterile soduim taurocholate solution. Rats from each group were killed at 12 h after sham operation or SAP and Kupffer cells (KCs) were isolated. The mRNA expressions of TNF-α and IL-1β (by quantitative real-time RT-PCR) and p38 MAPK activity (by Western blot analysis) in KCs were examined. The levels of TNF-α and IL-1β in plasma were determined by ELISA.RESULTS:There was a significant acvitation of p38 MAPK in KCs harvested from SAP rats than those from sham operation rats. SAP also promoted the mRNA expressions of TNF-α and IL-1β in KCs and the plasma levels of TNF-α and IL-1β. These events were significantly inhibited by treatment with CNI-1493.CONCLUSIONS:p38 MAPK activation is one important aspect of the signaling events that may mediate the KCs production of pro-inflammatory cytokines, TNF-α and IL-1β, in SAP rats. The inhibition of the p38 MAPK may be a potential target in the prevention and treatment of SAP.     

Genetic diversity in populations of the fungi Phaeomoniella chlamydospora and Phaeoacremonium aleophilum on grapevine in France

Isolates of Phaeomoniella chlamydospora ( Phc ) and Phaeoacremonium aleophilum ( Pha ), two haploid, deuteromycetous fungi, were obtained from vines showing symptoms of esca disease in different localities in two French regions, and within a single vineyard in one of these regions. The population genetic structure was determined in both fungi using random amplified polymorphic DNA (RAPD) analysis. Populations of Phc showed similar levels of diversity at local and regional levels. The most frequent Phc haplotypes were found in every population, and the frequencies of positive alleles of markers were similar across populations. The hypothesis that recombination had occurred was rejected for the full set of samples, but not for the samples reduced to haplotypes, indicating that Phc may be a recombining species. Different features were identified in Pha populations. First, the southern population of Pha appeared more diverse than the south-western populations. Second, genetic differentiation was identified between Pha populations from southern and south-western regions for several RAPDs. Finally, in the southern population of Pha no evidence for recombination was obtained, even by reducing the sample to haplotypes. Within the single vineyard surveyed, several haplotypes of both fungi were recovered and randomly distributed. Thus different infection events appeared to have occurred on a low spatial scale. Data from this study showed that haplotypes of both fungi were distributed over long distances geographically, and that most of the vineyards surveyed were infested by more than one haplotype of Phc and Pha .