Mesoscale spatial variability of ichthyoplankton in the Southwest Atlantic during the autumn–winter period

To gain a better understanding of the mechanisms underlying spatial heterogeneity in ichthyoplankton communities in the SW Atlantic, we examined for the first time the latitudinal and continental shelf–slope gradients in ichthyoplankton structure and oceanographic features in the Campos Basin during the relaxation phase of coastal upwellings (late autumn to early winter). This region, located on SE Brazil's continental margin, is the most productive offshore oil basin in the country and has ecological relevance owing to the existence of areas that experiences mesoscale (tens to hundreds of kilometers) processes (eddies, filaments, and upwelling) caused by the interaction of continental shelf and slope circulation with deep water masses. The present study collected a total of 3892 fish eggs and 10,030 larvae from 36 sampling stations, averaging 22 eggs per 100 m³ and 56 larvae per 100 m³. A total of 250 taxa (5 for fish eggs and 248 for larvae) were identified, encompassing 80 families and 145 genera. Species distribution exhibited a considerable degree of spatial variability, which was related mostly to hydrological characteristics. In general, greater densities were associated with higher nutrient concentrations areas. Through distance-based Redundancy Analysis, some discriminating species were found to associate with certain areas of the continental shelf characterized by colder temperatures. The ichthyoplankton distribution patterns suggested a potential influence from mesoscale oceanographic fronts, specifically those that induce upwelling of the cold and nutrient-rich South Atlantic Central Water. Nonetheless, the methodologies used in this study faced challenges in distinctly identifying these processes.     

乐清湾浮游动物现状调查研究

依据2011年5月和9月对温州乐清湾湾口附近海域浮游动物现状调查结果,分析和研究了浮游动物的种类组成、数量分布、多样性、群落结构及其与环境因子的相关性。5月份和9月份分别采获大型浮游动物13大类47种和16大类70种,调查海域附近浮游动物主要以近岸低盐类群为主,同时还有少量的半咸水河口类群。5月份浮游动物优势种为中华哲水蚤、球形侧腕水母、百陶箭虫等。9月份浮游动物优势种为背针胸刺水蚤、拿卡箭虫、汤氏长足水蚤、磷虾幼体等。5月份平均个体丰度为91个/m3,9月份平均个体丰度为115个/m3。5月份平均生物量为0.209 g/m3,9月份平均生物量为0.222 g/m3。9月份的丰富度指数、均匀度指数、多样性指数和优势度均高于5月份的各项群落结构指数。     

基于双年份三季相光谱混合分解的耕地变化遥感检测方法初步研究——以甘肃省民勤县为例

为切实保护耕地,基于遥感技术对不同耕地演化类型,包括不变耕地、新增耕地、撂荒耕地和退耕耕地进行识别和检测,以民勤县为例,采用2010和2015年2个年份的春、夏、初冬地物景观三季相特点的Landsat TM/OLI数据作为主要数据源。依据不同耕地演化类型的季相变异规律和内部要素特征选取具有物理意义的端元丰度值、丰度值差值建立2年份混合决策树划分不同耕地演化类型。同时对比分析在2010和2015年各自决策树土地利用/覆被分类结果后比较提取的耕地演化类型。结果表明:民勤县大部分耕地未发生变化,新增耕地在各个区域均有一定量的分布且主要分布在下游;退耕耕地大面积分布在坝区;撂荒耕地主要分布在沙漠绿洲过渡带。研究表明通过能够与地物先验知识关联的端元丰度值、特征端元丰度的差值建立决策树可以有效实现不同耕地演化类型的检测。误差矩阵精度评价结果显示,基于混合决策树耕地演化类型提取结果在总体精度上要高于分类后变化检测的结果。     

稻田土壤nirS型反硝化细菌群落对氮肥水平的响应

【目的】基于水稻田间定位试验,利用nirS功能基因研究不同氮肥水平对稻田土壤反硝化细菌群落多样性的影响。【方法】运用PCR-DGGE（聚合酶链反应-变性梯度凝胶电泳）结合DNA克隆测序和荧光定量PCR（Real-time PCR）技术对反硝化细菌nirS基因进行检测,分析田间定位试验第4年不同氮肥水平下稻田nirS型反硝化细菌群落结构和丰度的变化。【结果】依据DGGE图谱计算的群落多样性指数显示,与不施肥对照处理（CK）比较,施用氮肥处理（N1：75 kg N•hm-2,N2：150 kg N•hm-2和N3：225 kg N•hm-2）可促进稻田土壤nirS型反硝化细菌群落多样性指数提高,尤其在水稻生长的齐穗期和成熟期后者均显著高于前者（P＜0.05）。但群落多样性指数在N1、N2 和N3处理间的差异主要表现在水稻分蘖期和齐穗期的表层土壤中,N3可显著高于N1（P＜0.05）。冗余分析结果显示水稻生育时期对稻田土壤nirS型反硝化细菌群落结构的影响较大,表层和根层土壤的群落结构都与生育时期存在显著相关性（P=0.002,0.002）;而不同氮肥水平对群落结构的显著性影响仅表现在稻田表层土壤中（P=0.002）。荧光定量PCR结果显示氮肥水平提高可促进稻田土壤nirS型反硝化细菌丰度增加,在水稻分蘖期和齐穗期内表层和根层土壤的nirS基因拷贝数均存在CK＜N1＜N2＜N3的趋势,且以齐穗期时在表层土壤中的差异最大,不同处理间的差异都达到显著水平（P＜0.05）。同时,本研究获得稻田反硝化细菌nirS基因片段序列8条登录GenBank（登录号：JX997923、JX997924、JX997926—JX997931）。此外,本试验中N1、N2和N3处理比CK处理分别增产59%、92%和107%,表层和根层土壤中NO3--N含量也随氮肥用量提高而增加。【结论】氮肥用量增加促进了稻田土壤nirS型反硝化细菌丰度及群落多样性指数的提高,尤其在稻田表层土壤中。氮肥水平提高还可改变稻田表层土壤nirS型反硝化细菌的群落结构。综合分析表明稻田表层土壤的nirS型反硝化细菌群落对氮肥水平提高的响应程度更明显。     

安徽省油菜磷肥施用效果及土壤有效磷丰缺指标研究

为了解油菜磷肥施用效果及土壤有效磷丰缺指标,选择安徽省典型油菜产区,开展多年多点田间小区试验,设置不施磷和施磷2个处理,研究施用磷肥对安徽省冬油菜产量、增产效果和经济效益的影响,并通过建立油菜籽粒相对产量和土壤有效磷含量的关系,确定土壤有效磷丰缺指标。结果表明,油菜施用磷肥增产增收效果明显。油菜施磷后的产量平均为2 571 kg/hm~2,增产量和增产率分别为672 kg/hm~2和41.5%。施磷每公顷增收2 012元,89.6%的试验点产投比2.0,施磷收益显著。以相对产量60%,60%~75%,75%~90%,90%~95%和95%为标准,将土壤有效磷分成"严重缺乏"、"缺乏"、"轻度缺乏"、"适宜"和"丰富"5级,对应的丰缺指标为6.5、6.5~13.0、13.0~26.5、26.5~34.0和34.0 mg/kg。该研究结果为水稻田冬油菜的磷肥管理提供依据。     

县级测土配方施肥指标体系建立研究—以江苏省江都市水稻为例

以江苏省江都市水稻为例,基于地力差减法预测氮肥用量、 养分丰缺指标法预测磷钾肥用量,构建县域测土配方施肥指标体系,研究分析20052011年测土配方施肥田间试验示范。结果表明,当种植水稻预测目标产量为6825~9270 kg/hm2（平均为8145 kg/hm2）,氮肥推荐用量为146.42~57.4 kg/hm2（平均为207.5~ kg/hm2）。磷钾肥施用标准: 当有效磷含量高于21 mg/kg,不推荐施用磷肥;当有效磷含量在17~21 mg/kg时,推荐施磷（P2O5）量为40.5 kg/hm2;当有效磷含量在10~17 mg/kg时,推荐施磷（P2O5）量为 48 kg/hm2;当有效磷含量低于10 mg/kg 时,推荐施磷（P2O5）量为64.5 kg/hm2。当速效钾含量高于140 mg/kg,不推荐施用钾肥;当速效钾含量在115~140 mg/kg时,推荐施钾（K2O）量为 22.5 kg/hm2;当速效钾含量在60~115 mg/kg时,推荐施钾（K2O）量为40.5 kg/hm2;当速效钾含量低于60 mg/kg 时,推荐施钾（K2O）量为 82.5 kg/hm2。同时对施肥指标体系建立进行校验分析,结果表明基于试验建立施肥指标体系是可行的,从而为构建县域测土配方施肥指标体系提供了方法,达到因土施肥的目的。     

中亚热带丝栗栲群落物种-个体关系的研究

对中亚热带丝栗栲群落物种 个体关系研究结果表明 ,剩余标准差 (RSE)、偏差绝对值平均值 (AAD)和相对偏差绝对值平均值 (AARD) 3个评价指标之间存在极强正秩相关 ,表明它们对物种 个体关系模型评价结果具很强一致性 ,乔木层 5种曲线拟合效果最佳为y =1.85 18x2 - 7.84 86x 14 .5 ,灌木层 5种曲线拟合效果最佳为y =12 4 84e0 .1572x;群落中乔木层和灌木层物种多度分布均遵从对数正态分布 ,表明该丝栗栲群落发展较成熟 ,稳定性和生物多样性较高     

Isotopic fractionation during N2 fixation by four tropical legumes

To quantify the contribution of biological nitrogen fixation (BNF) to legume crops using the ¹⁵N natural abundance technique, it is necessary to determine the ¹⁵N abundance of the N derived from BNF—the B value. In this study, we used a technique to determine B whereby both legume and non-N₂-fixing reference plants were grown under the same conditions in two similar soils, one artificially labelled with ¹⁵N, and the other not. The proportion of N derived from BNF (%Ndfa) was determined from the plants grown in the ¹⁵N-labelled soil and it was assumed that the %Ndfa values of the legumes grown in the two soils were the same, hence the B value of the legumes could be calculated. The legumes used were velvet bean (Mucuna pruriens), sunnhemp (Crotalaria juncea), groundnut (Arachis hypogaea) and soybean (Glycine max) inoculated, or not, with different strains of rhizobium. The values of %Ndfa were all over 89%, and all the legumes grown in unlabelled soil showed negative δ¹⁵N values even though the plant-available N in this soil was found to be approximately +6.0‰. The B values for the shoot tissue (B_s) were calculated and ranged from approximately −1.4‰ for inoculated sunnhemp and groundnut to −2.4 and −4.5‰ for soybean inoculated with Bradyrhizobium japonicum strain CPAC 7 and Bradyrhizobium elkanii strain 29W, respectively. The B (B_wp) values for the whole plants including roots, nodules and the original seed N were still significantly different between the soybean plants inoculated with CPAC 7 (−1.33‰) and 29W (−2.25‰). In a parallel experiment conducted in monoxenic culture using the same soybean variety and Bradyrhizobium strains, the plants accumulated less N from BNF and the values were less negative, but still significantly different for soybean inoculated with the two different Bradyrhizobium strains. The results suggest that the technique utilized in this study to determine B with legume plants grown in soil in the open air, yields B values that are more appropriate for use under field conditions.     

青海省贵德县耕层土壤农化指标分析

[目的]评价贵德县耕层土壤养分状况。[方法]采用常规方法,分析贵德县499个耕层土壤8项农化指标。[结果]贵德县耕层土壤平均有机质19.71 g/kg,全氮1.54 g/kg,全磷1.70 g/kg,碱解氮111 mg/kg,有效磷41 mg/kg,速效钾249 mg/kg,缓效钾1 029 mg/kg,pH 7.97。碱解氮、速效钾、缓效钾总体含量水平高;有效磷、有机质含量与丰缺指标比较,结果为中等水平。[结论]该研究可为耕地地力评价和测土配方施肥提供基础数据。     

Modified Pollard transects for assessing tropical butterfly abundance and diversity

This paper introduces and discusses the consistency and effectiveness of an inexpensive modification of Pollard transects for assessing the diversity and abundance of tropical butterfly communities in two national parks in Rio de Janeiro, Brazil. To measure butterfly abundance, students walked simultaneous timed transects at the interface of forest and cleared areas. They either counted the number of individuals observed or tabulated the number of specimens collected with nets. After a short training period, the number of butterflies observed or collected on simultaneous transects was statistically indistinguishable among student groups, and there was a significant positive correlation between observation counts and collected number of individuals. As a measure of species richness, the number of butterfly species sampled on each simultaneous transect was tabulated and did not differ statistically. To measure diversity, alpha of the logseries model was calculated for each collected sample, and statistical fit to a logseries model was determined. Although virtually all daily samples and the year's accumulated sample at one park fit the logseries model, about 35% of the daily samples and the entire year's sample did not fit at the other park. Despite these differences between the two parks, values for alpha from daily samples at both parks varied similarly (from 15 to 50 in almost all cases), and values from the entire year's samples were statistically indistinguishable. The repeatability of results among novices, such as students, suggests that timed transects have great promise for furthering our understanding of butterfly community demographics.