全文获取类型
收费全文 | 12268篇 |
免费 | 619篇 |
国内免费 | 1233篇 |
专业分类
林业 | 129篇 |
农学 | 632篇 |
基础科学 | 30篇 |
313篇 | |
综合类 | 3812篇 |
农作物 | 425篇 |
水产渔业 | 809篇 |
畜牧兽医 | 5951篇 |
园艺 | 430篇 |
植物保护 | 1589篇 |
出版年
2024年 | 46篇 |
2023年 | 144篇 |
2022年 | 385篇 |
2021年 | 446篇 |
2020年 | 428篇 |
2019年 | 471篇 |
2018年 | 288篇 |
2017年 | 430篇 |
2016年 | 549篇 |
2015年 | 526篇 |
2014年 | 628篇 |
2013年 | 630篇 |
2012年 | 915篇 |
2011年 | 902篇 |
2010年 | 756篇 |
2009年 | 705篇 |
2008年 | 640篇 |
2007年 | 772篇 |
2006年 | 630篇 |
2005年 | 507篇 |
2004年 | 363篇 |
2003年 | 375篇 |
2002年 | 281篇 |
2001年 | 330篇 |
2000年 | 313篇 |
1999年 | 249篇 |
1998年 | 203篇 |
1997年 | 151篇 |
1996年 | 128篇 |
1995年 | 132篇 |
1994年 | 121篇 |
1993年 | 83篇 |
1992年 | 90篇 |
1991年 | 83篇 |
1990年 | 85篇 |
1989年 | 64篇 |
1988年 | 49篇 |
1987年 | 49篇 |
1986年 | 30篇 |
1985年 | 9篇 |
1984年 | 19篇 |
1983年 | 10篇 |
1982年 | 12篇 |
1981年 | 11篇 |
1980年 | 17篇 |
1979年 | 21篇 |
1978年 | 5篇 |
1977年 | 7篇 |
1956年 | 14篇 |
1955年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
71.
一个马铃薯Y病毒山东分离物的分离与鉴定 总被引:4,自引:1,他引:4
从具有典型花叶症状的马铃薯叶片中分离到马铃薯Y病毒(Potato virus Y,PVY)(本文称PVY-SD-TA分离物),扩繁后,提纯病毒,电镜下可观察到700~900 nm×11 nm的病毒粒体,病组织超薄切片观察可见风轮状的内含体结构,寄主反应特性研究表明其能侵染2科13种植物。SDS-PAGE电泳检测病毒编码的外壳蛋白亚基的分子量为33 kDa。以PVY-SD-TA基因组RNA为模板,应用RT-PCR方法和特异引物合成了外壳蛋白基因。对cDNA全序列分析表明,PVY-SD-TA CP基因核苷酸序列与N株系的同源性为96%,与GenBank中登录序列号为AJ390306的O株系分离物的同源性最高,为99%;与国内不同学者报道的PVY中国流行株的同源性分别为96%,97%和98%。通过以上生物学特性和分子水平的研究将PVY-SD-TA鉴定为普通株系(PVYO株系)。 相似文献
72.
利用RT-PCR检测库尔勒香梨苹果茎痘病毒的研究 总被引:3,自引:0,他引:3
以库尔勒香梨新鲜、冷藏、冷冻叶片和皮层为材料,对提取双链RNA (dsRNA)的2种方法和提取总RNA的3种方法进行了分析比较,并对总RNA的提取方法进行了改进,获得了纯度较高、完整性较好的dsRNA和总RNA,在此基础上进行了反转录(RT)和PCR扩增。在国内首次完成了对苹果茎痘病毒(ASPV)的RT-PCR检测,建立了ASPV有效RT-PCR反应体系。用此体系扩增到ASPV一个长约316 bp的片段。实验表明以dsRNA和总RNA为模板均能成功进行RT-PCR检测,且dsRNA优于总RNA。 相似文献
73.
74.
Martin M.T. Glie B. 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(5):427-431
Plum pox potyvirus (PPV) induces in infected Nicotiana clevelandii cells characteristic crystalline inclusions known as nuclear inclusions (NI) when located in the nucleus and as dense material (Dm) when located in the cytoplasm. Crystalline inclusions contain protease (NIa) and RNA-dependent RNA polymerase (NIb) proteins. It is now well established for all potyviruses that cylindrical inclusions contain CI helicase ATPase protein (Martin et al., 1992). The intracellular location of other non-structural PPV proteins remains unknown. Using Escherichia coli expression vectors, specific antibodies were obtained against P1, P3, 6K2 and NIb PPV proteins for which antibodies were not yet available. As expected, NIb antiserum labelled crystalline inclusions. P1, P3 and 6K2 proteins were present in both types of crystalline inclusions found in the nucleus and in the cytoplasm of PPV-infected leaves of N. clevelandii, suggesting that nuclear inclusions and dense material were composed of the same proteins. This composition is discussed. 相似文献
75.
B.C. Lee Y.K. He K. Murao M. Isogai G. Dahal I. Uyeda 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(6):493-499
Rice dwarf virus isolates were collected from several locations in Japan, the Philippines, China, Nepal and Korea. Genomic dsRNA segment profiles in polyacrylamide gel electrophoresis differed among the isolates. There were less differences in the profiles between isolates from Japan and Korea than in those between these two Countries and others. Nucleic acid hybridization was used to examine the extent of genomic variation. Full-length cDNAs to all genomic segments encoding non-structural proteins (S4, S6, S9, S10, S11 and S12) were synthesized from two Japanese isolates, and were used for dot-blot hybridization. Hybridizations using probes generated from the full-length cDNA clones failed to differentiate isolates from different geographical areas. However, cDNA probes covering a variable region of S12 were able to distinguish Japanese and Korean isolates from those of other countries. Phylogenetic tree analysis based on the amino acid sequence of P12 encoded by S12 grouped Japanese and Korean isolates together. The Chinese isolates from two different locations (Yunnan and Fujian) were closely related to each other, and were the most distantly related to Japanese and Korean isolates. 相似文献
76.
Y. Antignus M. Lapidot N. Ganaim J. Cohen O. Lachman M. Pearlsman B. Raccah A. Gera 《Phytoparasitica》1997,25(4):319-330
Received April 24, 1997; received in final form June 29, 1997. Symptoms resembling tomato spotted wilt virus (TSWV) infections
were documented among ornamental and vegetable crops in commercial greenhouses and open fields in Israel. Plants exhibiting
these symptoms were collected from January 1992 to December 1996. Among cultivated plants analyzed for TSWV by enzyme-linked
immunosorbent assay (ELISA), 19 species representing five families were found to be infected; natural infection was also recorded
in six plant species of weeds. Virus identity was characterized by host range, serology and electron microscopy. Serological
reaction with the isolates, found in Israel, using antisera from different sources as well as the sequence analysis of the
nucleocapsid gene, demonstrated that the Israeli isolates of TSWV are a member of tospovirus serogroup I, type I (BR-01 strain).
No virus transmission was found in seeds collected from virus-infected vegetable and ornamental crops. A non-radioactive molecular
probe derived from the cloned nucleocapsid isolate enables specific detection of the virus in crude sap from infected plants.
The detection of TSWV in Israel constitutes a severe potential threat to the ornamental and vegetable industry. 相似文献
77.
78.
79.
采用圆点印迹法检测GFkV,结果表明:Tris-HCl和PBS在提取GFkV时效果相同,在带毒葡萄植株中韧皮部的GFkV含量较高。 相似文献
80.