弱分枝西瓜wbl9的选育与初步应用

用具有弱分枝性状的wbl1作供体亲本,用优良的西瓜自交系015作父本,通过1次杂交、2次回交和连续6代的自交分离和纯化,育成了具有弱分枝性状的优良自交系wbl9。该自交系既保持了wbl1的弱分枝特性,又克服了wbl1不易坐果和品质与抗性较差的缺点,具体特征如下:平均每株含子蔓2.4个,种植可不整枝,省工省力;单株坐果0.93个,易坐果;果实圆形,单果重5～6kg,果实发育期31～33d;果皮绿色,上有墨绿色细齿带,薄而韧,耐储运;瓜瓤鲜红,质脆,中心可溶性固形物含量10.5%;田间抗性表现与015相近,但明显优于wbl1。用该自交系作亲本选育的新组合TH0401和TH0404综合性状表现优秀。结果表明,wbl9不仅可作为亲本材料直接应用,还可用作选育新弱分枝自交系的基础材料。     

大穗大粒冬小麦品种中麦9号的选育与推广

中麦 9号由中国农业科学院作物所麦类系高肥组以泗阳 936为母本、83鉴 2 5为父本 ,采用系谱法选育而成。北京农学院遗传育种研究所参与了示范和推广工作 .该品种具有矮杆、茎杆粗壮坚韧、抗倒伏、抗寒性较强、大穗大粒、综合农艺性状好、增产潜力大等突出特点。中麦 9号 1997年通过北京市和河北省审定 ;1998年通过全国审定 ,并被列入河北省十大推广品种 ;1999年获北京市科协金桥工程三等奖 .1996- 2 0 0 0年累计推广面积 10 0多万hm2 .     

优质高产糯玉米新品种渝糯9号选育

糯玉米杂交种渝糯9号是重庆市农科院以3469为母本,以S181为父本选育而成的。该品种具有品质优、产量高、中早熟、适应性广等特点,于2004年通过重庆市审定。     

2,4-二氯苯酚对鳞翅目昆虫细胞增殖的影响

研究了2,4-DCP对家蚕卵巢细胞(BmN)和苜蓿丫纹夜蛾卵巢细胞(Sf9)的影响.结果表明,2,4-DCP对BmN和Sf9细胞生长发育有很大影响,都表现为低浓度的2,4-DCP促进细胞增殖,超过一定浓度时表现出细胞毒性,导致培养细胞不贴壁生长和分裂,甚至死亡并收缩或破裂,有明显的浓度效应和时间效应.2,4-DCP对BmN和Sf9细胞的LC50分别为0.135和0.141 mmol/L.     

Effects of E2 binding enzyme UBC9 on porcine oocyte maturation,apoptosis and embryo development

SUMOylation is a dynamic post-translational modification process. However, the function of small ubiquitin-like modifiers (SUMOs) in the maturation of porcine oocytes and embryo growth is not well known. Therefore, the aim of this study was to investigate the effect of E2 binding enzyme UBC9 on the expression of SUMO-1 protein during the in vitro maturation of porcine oocytes and embryo development after in vitro fertilization. Four groups were used: 0 (Control), 5, 10 and 15 µg/ml UBC9. Western blotting, flow cytometry and RT-qPCR were used to detect the in vitro maturation of porcine oocytes, SUMO-1 content, viability and the expression of apoptotic genes. Compared to those in the control treatment, the maturation rate (p < .05) and viability (p < .01) of oocytes in the 5 μg/ml treatment group decreased significantly. SUMO-1 protein markers appeared at 59 and 71 kDa and the content of SUMO-1 protein in the 10 µg/ml treatment group decreased significantly (p < .05). In the expression of apoptosis-related genes, Bcl-2 gene expression was significantly downregulated in the 10 μg/ml treatment group (p < .05). However, Bax and Caspase-3 were significantly upregulated in the 5 μg/ml treatment group (p < .05). During embryonic development, the cleavage rate of oocytes in the 10 µg/ml treatment group was significantly reduced (p < .05), whereas blastocyst formation rate in the 5 µg/ml treatment group was significantly reduced. UBC9 regulates SUMO-1 content in mature pig oocytes in vitro, which affects oocyte maturation rate, viability, apoptotic genes expression and embryo development after fertilization.     

肌抑素对PK15细胞MMP-2/7/9表达的影响

本研究旨在揭示肌抑素（MSTN）与基质金属蛋白酶（MMPs）的调控关系,探明MSTN对PK15细胞MMP-2/7/9表达的影响。对MSTN单等位基因敲除猪背膘组织进行转录组测序分析,复苏前期制备的MSTN基因敲除PK15细胞系：单等位基因敲除的PK3108细胞系和双等位基因敲除的L18细胞系,通过实时荧光定量PCR和Wesrern blotting分别检测PK15、PK3108和L18细胞系中MSTN、MMP-2/7/9基因的mRNA和蛋白表达水平。结果发现,与野生型猪相比,MSTN单等位基因敲除猪背膘组织转录因子C/EBPδ、MMP-2/7基因mRNA表达量均极显著下调（P < 0.01）;细胞外基质中纤连蛋白（FN）和层连接蛋白（LN）含量均极显著增加（P < 0.01）。复苏的PK3108和L18细胞呈现绿色荧光。实时荧光定量PCR结果显示,PK3108和L18细胞中MSTN、MMP-2/7/9的mRNA表达量均极显著低于PK15细胞（P < 0.01）;Western blotting结果显示,PK3108和L18细胞中MSTN、MMP-2/7/9的蛋白表达量均明显低于PK15细胞。本研究结果表明,在MSTN基因敲除的PK15细胞中,MSTN功能缺失能显著降低MMP-2/7/9的表达,且MMP-2/7/9蛋白表达降低的趋势与MSTN蛋白表达的趋势相一致。     

B9作为矮化剂生产案头菊的研究

本文探讨了案头菊的内涵，设立了有关的园艺指标。经过６年的试验，采用５０００ｍｇ·Ｌ＾－１的Ｂ９作为矮化剂，从６６个中国晚菊品种中筛选出了１６个适合用来生产案头菊的中国晚菊品种，总结出了一套适合案头菊生产的技术措施。其具体做法是：每年５月至７月采用扦插法育苗，在定植后间隔６￣１０ｄ进行叶面喷洒５０００ｍｇ·Ｌ＾－１的Ｂ９直至现蕾。由于品种差异，供试材料对Ｂ９的反应各不相同。使用Ｂ９会使案头菊花期后延     

灌溉对柱花草和坚尼草产量与品质的影响

对柱花草（Stylosanthes guianensis）与坚尼草（Panicum maximum CIAT 9)2种热带牧草在旱季进行人工灌溉后刈割,测定灌溉和不灌溉对生物量的影响。分析了2种热带牧草在人工灌溉条件下生物量的变化,结果表明,在人工灌溉条件下生长的2种热带牧草,生长速率快,刈割周期短,其产量和营养价值也有所不同。坚尼草在灌溉条件下其鲜生物量为不灌溉的1.4倍,生长周期缩短34%;柱花草在灌溉条件下其鲜生物量为不灌溉的1.06倍,生长周期缩短27%。     

H9N2亚型猪流感病毒诱导小鼠肺急性损伤模型的研究

本试验采用100 μLA/swine/HeBei/012/2008/(H9N2)猪流感病毒(H9N2 SIV)经鼻腔接种6～8周龄的BALB/c小鼠,通过定时称量小鼠的采食量和体质量、观察肺病理组织学变化以及测量肺系数、肺湿质量/干质量、动脉血气和支气管肺泡灌洗液内炎性细胞等拟建立H9N2 SIV感染诱导小鼠急性肺损伤模型.结果显示:(1)感染后第2天试验组小鼠出现精神沉郁、被毛松乱、采食量和体质量下降.感染后的第3天开始感染小鼠采食量和体质量显著下降(P＜0.01);(2)试验组小鼠在感染后的第3天末出现死亡,死亡率约为62.5%,剖检可见肺部明显水肿、出血,肺泡内有大量的炎性细胞渗出;肺系数和湿质量/干质量比显著增加(P＜0.01);(3)与对照组相比感染组小鼠动脉血中氧分压从第2天开始降低,第4天出现明显的差异(P＜0.01),第6天最低时仅为(6.79士1.27)kPa,呈现严重的低氧血症,同时,二氧化碳分压显著上升;(4)支气管灌洗液(BALF)内炎性细胞在感染后第4-8天增加显著,尤其以肺泡巨噬细胞和多核型白细胞增加最为明显(P＜0.01).本研究证实采用A/Swine/HeBei/012/2008/(H9N2)病毒感染小鼠引起肺组织弥漫性急性渗出性炎症为主的病理过程和严重的低氧血症,表明成功建立了H9N2 SIV诱导小鼠的肺损伤模型,为进一步研究其对哺乳动物肺损伤的致病机理奠定基础.     

Seroepidemiologic survey for Coxiella burnetii among hospitalized US troops deployed to Iraq

Q fever is a zoonotic illness which frequently has a non-specific clinical presentation. Cases among deployed US military personnel have been reported in increasing numbers indicating an emerging at-risk occupational group. Banked serum specimens were utilized to estimate seroprevalence and risk factors among military personnel deployed to Iraq. Coxiella burnetii antibody testing was performed and epidemiologic data were analysed from 909 servicemembers. The overall number who seroconverted to Q fever was 88 (10%). The most common ICD-9 code assigned to Q fever cases was fever not otherwise specified (NOS) (45%). A combat occupational specialty was a risk factor for Q fever seroconversion (OR = 1.8, 95% CI: 1.1-2.8) as well as receiving a primary diagnosis of fever NOS (OR = 2.6, 95% CI: 1.6-4.1). These findings indicate that Q fever is a significant infectious disease threat to military personnel deployed to Iraq. A heightened awareness among physicians is necessary to ensure prompt diagnosis and treatment.