2,4-二氯苯酚对鳞翅目昆虫细胞增殖的影响

研究了2,4-DCP对家蚕卵巢细胞(BmN)和苜蓿丫纹夜蛾卵巢细胞(Sf9)的影响.结果表明,2,4-DCP对BmN和Sf9细胞生长发育有很大影响,都表现为低浓度的2,4-DCP促进细胞增殖,超过一定浓度时表现出细胞毒性,导致培养细胞不贴壁生长和分裂,甚至死亡并收缩或破裂,有明显的浓度效应和时间效应.2,4-DCP对BmN和Sf9细胞的LC50分别为0.135和0.141 mmol/L.     

三元配合物[La( C9H6NO)2(C6H5COO)]·H2O浸种对水稻种子萌发及幼苗生长的影响

[目的]研究[La（C9H6NO）2（C6H5COO）].H2O浸种对水稻种子萌发及幼苗生长的影响。[方法]采用不同浓度（0.005×10-3、时,则抑制种子萌发及幼苗生长。[结论]适当浓度的[La（C9H6NO）2（C6H5COO）].H2O可促进种子萌发及幼苗生长。0.010×10-30、.050×10-3、0.100×10-3、0.300×10-3 mol/L）的[La（C9H6NO）2（C6H5COO）].H2O水溶液处理水稻种子。[结果][La（C9H6NO）2（C6H5COO）].H2O浓度为0.005×10-3～0.100×10-3mol/L时,促进种子萌发及幼苗生长,浓度大于0.300×10-3 mol/L时,则抑制种子萌发及幼苗生长。[结论]适当浓度的[La（C9H6NO）2（C6H5COO）]·H2O可促进种子萌发及幼苗生长。     

H9N2亚型禽流感病毒A/Chicken/Shandong/LY1/2017株反向遗传操作系统的建立

为了构建H9N2亚型禽流感病毒A/Chicken/Shandong/LY1/2017的反向遗传操作平台,通过RT-PCR技术分别扩增该毒株的8个基因片段,并分别克隆至双向转录表达载体PHW2000中.8个质粒共转染293T/MDCK混合细胞,96 h后将细胞板反复冻融3次收获细胞悬液,将其接种10日龄SPF鸡胚,96 h后收集尿囊液并将拯救毒株命名为rH9N2,测定其HA效价为1:256.rH9N2在HA、MDT、EID50、TCID50以及病毒生长曲线、空斑等方面保持了与亲本毒株一致的生物学特性.本研究成功构建了A/Chicken/Shandong/LY1/2017的感染性克隆,为今后通过基因替换、定点突变技术研究H9N2亚型禽流感病毒致病性的分子机制奠定了基础.     

丰产抗病西瓜新品种农科大9号的选育与评价

为了选育丰产性、抗病性更强的西瓜新品种,以高抗枯萎病母本Z10-1和优良父本J2-2杂交配制农科大9号。该品种中晚熟,露地栽培全生育期约100 d,保护地栽培全生育期120 d,果实发育期34 d。植株生长势强,坐果容易且整齐。果实椭圆形,果型指数1.4。果皮绿色,上覆深绿色中细条带。果肉红色,肉质脆,果实中心可溶性固形物含量95 mg/g、总糖85.3 mg/g、蛋白质11.1 mg/g、粗纤维0.71 mg/g、Vc 0.059 1mg/g。单瓜质量7.47 kg。区试产量66 073.5 kg/hm2,生产示范产量75 180 kg/hm2,分别比对照西农8号增产9.8%和16.2%。抗枯萎病性强于对照。     

早熟春大豆新品种赣豆9号的选育及配套栽培技术

赣豆9号是江西省农业科学院作物研究所以油春01-49为母本,沔035为父本进行有性杂交,经多代系谱法选育而成的早熟、优质春大豆新品种。2014-2015年参加江西省春大豆区域试验,两年平均产量1 960.5 kg·hm^-2,较对照中豆40增产3.06%,春播全生育期88 d,粗蛋白含量为44.6%,粗脂肪含量为19.1%,粗蛋白质与粗脂肪总含量为63.7%,2016年通过江西省农作物品种审定委员会审定,适宜在江西省大豆产区做春大豆种植。     

甬优9号产量构成因素相关分析及高产栽培技术

通过调查甬优9号作单季中稻种植不同产量水平下的产量构成及相关分析,探讨各产量因子间内在的联系及对产量的影响。结果表明,每穗粒数与产量的相关程度最密切,其次是结实率和有效穗,千粒重比较稳定,与产量的相关不密切。生产上应在确保高产所需有效穗的基础上,主攻每穗粒数,提高结实率,并兼顾粒重,为甬优9号高产栽培的有效技术途径。     

Vibrio vulnificus MO6-24/O Lipopolysaccharide Stimulates Superoxide Anion,Thromboxane B2, Matrix Metalloproteinase-9, Cytokine and Chemokine Release by Rat Brain Microglia in Vitro

Although human exposure to Gram-negative Vibrio vulnificus (V. vulnificus) lipopolysaccharide (LPS) has been reported to result in septic shock, its impact on the central nervous system’s innate immunity remains undetermined. The purpose of this study was to determine whether V. vulnificus MO6-24/O LPS might activate rat microglia in vitro and stimulate the release of superoxide anion (O₂⁻), a reactive oxygen species known to cause oxidative stress and neuronal injury in vivo. Brain microglia were isolated from neonatal rats, and then treated with either V. vulnificus MO6-24/O LPS or Escherichia coli O26:B6 LPS for 17 hours in vitro. O₂⁻ was determined by cytochrome C reduction, and matrix metalloproteinase-2 (MMP-2) and MMP-9 by gelatinase zymography. Generation of cytokines tumor necrosis factor alpha (TNF-α), interleukin-1 alpha (IL-1α), IL-6, and transforming growth factor-beta 1 (TGF-β1), chemokines macrophage inflammatory protein (MIP-1α)/chemokine (C-C motif) ligand 3 (CCL3), MIP-2/chemokine (C-X-C motif) ligand 2 (CXCL2), monocyte chemotactic protein-1 (MCP-1)/CCL2, and cytokine-induced neutrophil chemoattractant-2alpha/beta (CINC-2α/β)/CXCL3, and brain-derived neurotrophic factor (BDNF), were determined by specific immunoassays. Priming of rat microglia by V. vulnificus MO6-24/O LPS in vitro yielded a bell-shaped dose-response curve for PMA (phorbol 12-myristate 13-acetate)-stimulated O₂⁻ generation: (1) 0.1–1 ng/mL V. vulnificus LPS enhanced O₂⁻ generation significantly but with limited inflammatory mediator generation; (2) 10–100 ng/mL V. vulnificus LPS maximized O₂⁻ generation with concomitant release of thromboxane B₂ (TXB₂), matrix metalloproteinase-9 (MMP-9), and several cytokines and chemokines; (3) 1000–100,000 ng/mL V. vulnificus LPS, with the exception of TXB₂, yielded both attenuated O₂⁻ production, and a progressive decrease in MMP-9, cytokines and chemokines investigated. Thus concentration-dependent treatment of neonatal brain microglia with V. vulnificus MO6-24/O LPS resulted in a significant rise in O₂⁻ production, followed by a progressive decrease in O₂⁻ release, with concomitant release of lactic dehydrogenase (LDH), and generation of TXB₂, MMP-9, cytokines and chemokines. We hypothesize that the inflammatory mediators investigated may be cytotoxic to microglia in vitro, by an as yet undetermined autocrine mechanism. Although V. vulnificus LPS was less potent than E. coli LPS in vitro, inflammatory mediator release by the former was clearly more efficacious. Finally, we hypothesize that should V. vulnificus LPS gain entry into the CNS, it would be possible that microglia might become activated, resulting in high levels of O₂⁻ as well as neuroinflammatory TXB₂, MMP-9, cytokines and chemokines.     

玉米新品种漯单9号的高产稳产性分析

对2002～2004年河南省玉米区域试验、生产试验及黄淮海夏玉米区域试验结果分析表明,漯单9号属高产、稳产型玉米新杂交种,广泛适宜于黄淮海夏玉米区推广种植。由于其子粒品质优良,推广开发前景十分广阔。     

盐胁迫下四个水稻类受体蛋白激酶的功能分析

[目的]盐胁迫是制约水稻生长和产量主要逆境之一,研究盐胁迫响应基因对于了解植物耐盐机理和培育耐盐水稻品种具有重要意义.类受体蛋白激酶RLK(receptor-like protein kinases)广泛参与调控植物细胞信号转导和对逆境胁迫的响应过程.本研究的目的是分析盐胁迫下四个RLK基因的表达模式和生物学功能.[方...     

铝胁迫下一氧化氮对巨尾桉生长及生理的影响

酸铝抑制植物生长是全球关注的热点。以往关于酸铝胁迫的研究主要在禾本科和豆科植物上,而对经济用材林木较少。为此,本研究以巨尾桉广林9号为供试材料,在无铝（0 mmol/L）和铝胁迫（5 mmol/L）2种情况下,设置一氧化氮（NO）3个水平（0、10、500 μmol/L）,以期探究铝胁迫下外源施加NO对巨尾桉生长和生理方面的影响。试验结果表明：（1）5 mmol/L铝处理下的巨尾桉根、茎、叶生物量分别显著减少了11.57%、12.04%和37.37%,叶片叶绿素a、叶绿素a+b和类胡萝卜素含量积累也受抑制;（2）铝胁迫下叶片细胞膜透性显著增大,膜脂过氧化的产物丙二醛（MDA）显著增加了43.12%,细胞可溶性糖和蛋白含量分别显著减少了9.43%、42.27%;（3）适量添加NO （10 μmol/L）可提高铝胁迫下光合色素含量,提高最大的叶绿素a达41.23%,MDA含量明显降低了27.66%,提高可溶性糖和可溶性蛋白含量,以达到调节活性氧代谢来维持细胞膜结构稳定的效果,从而使生物量显著提高了10.19%;（4）NO浓度过高（500 μmol/L SNP）时反而对巨尾桉有毒害作用,生物量下降了15.17%,说明NO具有双重性。综上所述,铝胁迫对巨尾桉生长、渗透调节物质和抗氧化酶等生理特性均产生了抑制作用,而适当添加外源NO可以缓解铝对巨尾桉的毒害作用。