Fermentation,formulation and evaluation of PGPR Bacillus subtilis isolate as a bioagent for reducing occurrence of peanut soil-borne diseases

Four isolates of Bacillus subtilis coded, B4, B7, B8 and B10 were examined as biocontrol agents for their abilities and antagonistic effect on the in vitro growth of certain phytopathogenic fungi of peanut, Rhizoctonia solani and Sclerotium rolfsii. Bacillus subtilis isolate B4(GenBank accession no. EF150884) was the highly effective one for inhibiting the fungal mycelial growth. Batch fermentation of B. subtilis isolate B4 was carried out and the maximum biomass achieved was 4.53 g L~(-1) at 11 h. Bacillus subtilis isolate B4 was formulated and evaluated as a biofungicide to reduce peanut soil-borne diseases under greenhouse and field conditions at the side of Rizolex-T(fungicide) as standard. Treatments by formulated plant growth-promoting rhizobacteria(PGPR) B. subtilis B4 and Rizolex-T in a soil infested with R. solani, S. rolfsii and mixture of them were more effective in decreasing percentage of damping-off, root and pod rot disease incidence(%) in greenhouse and open field environment during the two seasons 2015 and 2016. Treatments by PGPR gave highly dry weight and number of healthy pods compared to control of fungi treatment which was nearby to dry weights of healthy pods achieved by treatments by Rizolex-T in a soil infested with S. rolfsii, R. solani and mixture of them. Formulated PGPR B. subtilis B4 gave higher increasing of yield percentage than treatment by Rizolex-T in the two evaluated seasons 2015 and 2016. It can conclude that the produced bioforumlated agent was more efficient as fungicide when compared with the other chemical synthesized fungicides, safe for human and the environment and economy.     

火炬松干枯型枯梢病的发生与防治技术研究

研究表明，火炬松干枯型枯梢病始于６月，在７～９月的高温干旱期，病害较严重，但整个病变过程比较缓慢。１９９３～１９９６年防治试验说明，５～６月，土壤或叶面施硼砂、微肥复合晶、钼酸铵、柑桔微肥，防治效果好。尤其是硼砂，防效高，肥效稳定。土壤施硼砂２３～２５ｇ／株，１ａ一次；叶面喷０．２％的硼砂水溶液，连喷３次，可以在生产上推广应用。     

烟草种质资源TI203对烟草花叶病的抗性特点

[目的]系统了解烟草种质资源TI203抗烟草花叶病毒(Tobacco mosaic virus,TMV)的特点,为烟草抗病育种的亲本选择提供更丰富信息.[方法]以烟草种质资源TI203为材料,采用TMV病毒接种、病情调查、病毒外壳蛋白累计量检测、TMV复制水平测定及日灼现象观察等方法,研究TI203对TMV的抗性特异性、TI203接种TMV普通株系(TMV-U1株系)后的症状、病毒复制水平等.[结果]TI203只对TMV表现无症状的抗病特点,接种马铃薯Y病毒(PVY)和黄瓜花叶病毒(CMV)则表现出典型症状.接种TMV-U1株系后14 d,Western blotting检测结果显示,TI203叶片病毒复制水平明显低于对照品种;用表达GFP的TMV侵染性克隆浸润接种发现,TI203对TMV的抗性发生在病毒复制水平.在高温和强日照条件下,TI203生长迅速的中上部叶片会发生日灼现象,且难以恢复.[结论]TI203只对TMV表现无症状,其对TMV的抗性发生在病毒复制水平.     

高产优质晚粳新品种‘宝农203’的选育及特性研究

为选育高产优质水稻新品种,宝山区农业良种繁育场以‘武运粳7号’与‘宝农14’杂交F₁代花粉经单倍体生物组培选育成中熟晚粳品种‘宝农203’;其株高105 cm,有效穗210万～300万穗/hm²,每穗总粒数145～155粒,结实率90%,千粒重25～26 g,全生育期约160 d;其特点是高产、米质优良、综合抗性好,适宜于长三角地区及相似生态环境类型水稻区种植。     

渝香203在重庆低海拔高产栽培的适宜田间配置方式研究

为明确优质水稻新品种渝香203在重庆低海拔高产栽培的适宜田间配置方式,采用大田试验研究了田间配置方式对水稻茎蘖消长、干物质生产与分配、中后期叶面积指数及剑叶SPAD值、产量及产量构成的影响.结果表明,扩行稀植的方法不仅有利于茎蘖的发生和有效茎蘖数的提高,还有利于增加水稻单株干物质积累量.高产水稻群体齐穗期的地上部干质量为成熟期穗干质量的1.5倍左右,齐穗期主茎5片叶表现为“2＞3≥1≥4 ＞5”的叶长排序.推荐的田间配置方式为26.6 cm×33.3 cm或23.3cm×33.3 cm,种植密度为7500～ 8570穴/666.7 m2,每穴栽插2粒谷苗.     

油葵赤葵杂1号、KWS203复种播期试验研究

试验在相同栽培管理条件下,观察赤葵杂1号、KWS203两个油葵品种在科尔沁区4个不同播期的生育进程、生育性状,并根据它们的产量、含油量确定了复种油葵杂交种的适宜播种时间.     

关于人造板高耐磨固化液体表层的探讨

人造板表现高耐磨处理工艺,是当今人造板精细加工工业发展的首要方向。本文所探讨的高耐磨固化液体表层的 是将表层的所有组份按比例制成液体形态,使每个耐磨颗粒被胶液包裹着,均匀地分布于胶层的各个层面中,级固化后,从而生产更高的耐磨强度,它改变了传统工艺中耐磨颗粒只能不均匀地置于表层纸最外层的弊端,大幅度地提高了耐磨表层的质量,降低了生产成本。     

Expression of miR-203 in human tongue carcinoma tissues and its in-fluence on viability and invasion ability of Tca8113 cells

AIM: To study the expression of miR-203 in tongue carcinoma tissues and the effect of miR-203 over-expression on the viability and invasion ability of Tca8113 cells.METHODS: Twenty-eight pairs of tongue carcinoma tissues and adjacent nontumor tissues were collected, and the clinicopathological characters were analyzed. miR-203 was detected in the tongue tissues of 28 patients with tongue carcinoma by real-time PCR. miR-203 mimics and scramble were transfected into Tca8113 cells by Lipofectamine 2000. The expression of miR-203 was detected in Tca8113, Tca8113-miR-203 mimics and Tca8113-scramble cells by RT-qPCR. The cell viability was measured by CCK-8 assay. The cell invasion ability was determined by Transwell chamber invasion experiment.RESULTS: miR-203 expression was significantly down-regulated in the tongue carcinoma tissues compared with those in the adjacent nontumor tissues. The expression of miR-203 was associated with TNM stage and lymph node metastasis. Up-regulation of miR-203 inhibited the viability and invasion ability of Tca8113 cells.CONCLUSION: miR-203 suppresses the growth and invasion of tongue carcinoma cells. miR-203 may be a potential therapeutic target for treating human tongue cancer.     

浙中山区鲜食红辣椒品种比较试验

对引进的4个小红椒品种植株性状、果实性状、产量等方面进行比较试验,结果表明,红天湖203和红丰404,产量高,椒果外表光亮、顺直,后期不变形,植株高大,结果期长。特别是红天湖203香辣味更强,抗病性好,深受市场欢迎,可在浙中山区磐安县及气候相似地区作鲜食小红辣椒示范推广。     

不同栽培处理下杂交水稻渝香203的再生性分析

重庆是再生稻的主产区之一,积极发展再生稻生产对确保粮食安全,改善稻米结构、增加农民收入、满足城乡人民生活需求等都具有重要意义.手工栽秧、机栽秧、抛秧、直播是目前水稻的主要栽培方式.本研究设置了人栽人收、人栽机收、机栽人收、机栽机收、抛秧人收、直播人收6个处理,比较了不同栽培处理下杂交水稻的再生性、再生稻产量及中稻-再生稻产量.结果表明:人栽人收在再生性、再生稻产量、中稻-再生稻总产上都具有明显的优势,依次为人栽机收、机栽人收、机栽机收、抛秧人收,直播人收处理下表现最低.