甘肃省“121”雨水集流工程经济

甘肃中东部地区地处黄土高原，气候干旱，水资源缺乏，农村人畜饮水困难。“121”雨水集流工程的实施，有效地解决了这些地区的人畜饮水困难。为了客观评价“121”雨水集流工程的经济效益和作用，利用建设项目后评价理论与方法，对工程的经济效益进行了科学评价，国民经济评价结果可行，其结果可用于指导全省雨水利用工程的规划、设计，加强雨水利用工作的决策、管理。     

灌溉渠系流量最优调配的0-1规划模型

本文根据灌溉配水渠系的运行特点,提出在一定轮期和来水流量限制条件下实现下级配水渠最优组合及其配水时间最优排序的一种0-1规划模型,实例运算表明,这种方法可有效减少无效弃水和闸门调节次数,同时对渠道横断面的设计有参考意义。     

紫外辐照对产α-ALDC枯草芽孢杆菌的诱变效应

采用紫外诱变(波长260nm，功率15w)对一株产α-乙酰乳酸脱羧酶的枯草芽孢杆菌（Bacillus subtilis）BS059菌株进行诱变处理。结果表明，在紫外辐照时间为50s时，诱变效果较好。从正突变菌株中反复筛选，得到两株产酶量较高的菌株BS059-15和BS059-22，比原出发菌株的酶活分别提高了107.62%和162.25%。经过连续传代试验，证明其遗传性状稳定，为可遗传变异。     

Analysis of Application of Information in Mid - mini Enterprises on Condition of Mass- economy

The group of Mid - mini enterprises under mass - economy is the intermediate organization built up by specialized division of work and cooperation. This kind of organization is flexible in operation with high efficiency, but difficult in development of new technology. On the basis of analyzing the character and deficiency of the mid - mini enterprises, in this paper, through providing a framework of information service platform for the mid - mini enterprises to enhance their creative ability and complex competition. The construction and operational strategy of such platform are investigated. Finally a successful case study is given.     

Energy Efficiency Analysis of Heating System in the Birdhouse

In this paper the problem of energy consumption and environment in the birdhouse is introduced and the reconstructive measures aiming at energy savings are recommended. The measures include the limitation of temperature region, reconstruction of the building envelop and using the sunny board at the south side of the birdhouse roof. Finally, the energy efficiency of the reconstructive project is estimated.     

Discussion about the Agent- Construction System of Governmental Project in China

The agent - construction system, as a new public project management mode, has been practiced in some cities and attracted attention from the society. This paper firstly studies the features and operation modes of agent - construction system, and discusses the agent - construction system from the viewpoints of promoting competition, nourishing agent -construction enterprises, setting up a supervisory and encouraging mechanism, and its connection with the governmental project procurement system etc.     

Nonlinear FEM Analysis of RC Staggered Joint by Using APDL Language

In order to settle some problems involved in applying ANSYS into nonlinear Finite Element Analysis of RC structure,this paper discusses the nonlinear FEM method for staggered joint,incorporating its experimental result.The main solved problems are as follows:(1) The fundamental process of nonlinear FEM analysis;(2) How to model the behaviors of reinforcement and concrete respectively;(3) How to model the bond-slip relationship between reinforcement and concrete.     

Application of Water Quality Standand Leuel Method in Eualuazing Status Quo of Heauy-Metals Pollution in Riuers

The status quo of heauy-metals pollution in water phase of course of Long-Tan hydropower statian was eualuated by water Quality Standand Level Method. With this special method,mone objectiue and definite results were obtained.     

A MULTI -OBJECTIVE FUZZY DECISION -MAKING APPROACH FOR POWER NETWORK PLANNING UNDER UNCERTAINTY

This paper proposes a multi - objective fuzzy decision - making approach for power network planning under uncertainty ,which simutaneously considers not only the least investment cost,the minimum power loss,the maximum reliability,and the least environmental impacts, but also the uncertainty about the future load growth and the capital investment availability. The validity and effectiveness of the proposed approach is verified with numerical examples.     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.