抑制素免疫对牛、羊繁殖力作用的研究进展

文章综述了天然提纯,化学合成及重组等不同种类抑制素抗原免疫对牛、羊繁殖力的影响.近年来抑制素疫苗作为多胎疫苗的角色已越来越为人们所重视,其免疫原性同抗原本身一些因素如纯度、免疫原性、异原性、免疫剂量有关.     

胰岛素样生长因子及其受体mRNAs在绵羊生殖系统中的表达和分布

为检测胰岛素样生长因子（IGFs）及其受体（IGFR）mRNAs在绵羊发情周期早期卵巢、子宫和输卵管中的表达,探讨绵羊胚胎早期发育过程中其发育环境——生殖道中生长因子的表达、分泌及其作用,取绵羊发情周期早期卵巢、子宫和输卵管,经固定、切片、免疫染色,观察IGFs mRNAs的表达和分布情况。同时用RT-PCR技术研究了各组织中IGF-Ⅰ、IGF-Ⅱ、IGF-ⅠR、IGF-ⅡR mRNAs的表达情况。结果表明,IGFs mRNAs在绵羊发情周期早期的卵巢、子宫和输卵管中都有表达,4种因子表达模式相似：在卵巢中,IGFs主要定位于卵泡颗粒细胞,间质细胞亦有少量表达。在输卵管中,上皮细胞免疫染色呈阳性;在子宫中,腺细胞及上皮细胞的阳性信号强于固有层。RT-PCR检测表明IGFs mRNAs在3种组织中均有表达。     

新疆国际客源市场时空发展变化分析研究

利用集中度和亲景度、竞争态指标,对新疆国际客源市场13个主体客源国1997～2004年统计数据进行分析,揭示国际客源时空演替规律,指出新疆主要的3个国际客源市场是日本、俄罗斯和美国,并提出了拓展国际客源市场的建议。     

应用灰色系统理论评价草地质量初探

以草地优良牧草产量、可食牧草产量、载畜量、牧草利用率和覆盖度为主要指标，应用灰色系统理论中关联工分析的方法，对武威地区３２个类型草地质量进行了评价。结果表明，质量最好的草地是山地草甸，质量最关的是干荒漠和荒漠摹。根据分析结果，提出了武威地区草地质量分级的方案。     

杉木外地优良家系引种及选择研究

从南京林业大学引进22个杉木(Cunninghamia lanceolata)第二代种子园优良家系,对其10年试验结果进行分析,引种家系胸径生长与对照存在显著的遗传差异,大部分家系生长量不如贵州黎平的(对照ck2)高;各家系生长量达显著和极显著水平,以生产力指数、稳定性参数和材积生长量综合评判,将参试家系分成优良(Ⅰ)、中等(Ⅱ)、较差(Ⅲ)3类,并从中选出3个可以在我省利用和推广的优良家系。     

Expression and Bioinformatic Analysis of ADIPOQ Gene in Hanper Sheep

The aim of this study was to investigate the expression and biological characteristics of adiponectin (ADIPOQ) gene in longissimus dorsi muscle of Hanper sheep at different months of age.The ADIPOQ gene mRNA expression was detected in longissimus dorsi muscle of Hanper sheep at 3 different growth periods (1,7 and 13 months old) by Real-time quantitative PCR.The bioinformatics method was used to analyze the nucleotide and amino acid sequences of ADIPOQ gene,protein characteristics and network interaction protein.The results showed that ADIPOQ gene was expressed in longissimus dorsi muscle of Hanper sheep,which showed an increasing trend with the growth of the months age without significant difference (P>0.05).There was higher homology of ADIPOQ gene with Capra hircus (98.60%,99.00%) and Bos taurus (98.60%,87.00%) in nucleotide and amino acid sequences.The results of physical and chemical properties analysis showed that the molecular formula of ADIPOQ protein was C₁₁₇₂H₁₇₇₆N₃₁₄O₃₄₉S₅,the molecular mass was 26.00 ku,and the theoretical isoelectric point (pI) was 5.88,which suggested that the protein was acidic.The average value of ADIPOQ hydrophilic protein (GRAVY) was －0.403,which indicated that the protein was hydrophilic and didn’t belong to transmembrane protein.In addition,the signal peptide sequence was Met¹-Ala¹⁷,and the cleavage site was between Ala¹⁷ and Arg¹⁸.ADIPOQ existed collagen structure domain and C1Q conserved structure domain at position 45－101 and 101－237,respectively.The secondary structure mainly composed of random coil (66.95%).The construction of protein interaction network indicated that ADIPOQ might interact with ADIPOR1,ADIPOR2,CDH13 and LEP.The results provided a theoretical basis for further exploring the molecular biological functions of ADIPOQ gene in the process of muscle development in Hanper sheep.     

新疆生产建设兵团报、刊文献资源的研究与利用

记述兵团报、刊历史发展的概况,分析了兵团报、刊文献资源所反映的军垦文化特色,并对其开发利用进行研究。     

BTH对新疆甜瓜过氧化物酶的系统诱导作用

采用3种浓度(25,50,100μg·ml-1)的BTH对耐病品种皇后和感病品种早金的第一片真叶进行诱导处理后,经诱导后不同时间测定第一片和第二片真叶的过氧化物酶(POD)活性,结果表明:BTH的三种不同的浓度处理均能诱导出耐病品种皇后第一片和第二片真叶的过氧化物酶(POD)活性的升高,与空白差异显著,以25μg·ml-1BTH处理的过氧化物酶(POD)活性最高,空白的为最低。第一片真叶的酶活性高峰为诱导处理后的第7天和第16天,第二片真叶的酶活性高峰为诱导处理后的第16天。BTH的三种不同的浓度处理也能诱导出感病品种早金第一片和第二片真叶的过氧化物酶(POD)活性的升高,但与空白差异不显著。以25μg·ml-1BTH处理的过氧化物酶(POD)活性最高,空白的最低。证明了甜瓜抗病性与甜瓜植株过氧化物酶活力呈正相关。BTH诱导酶活性的效应可以通过植物的输导组织,传导给其它未经处理的甜瓜组织,诱导其它组织中的过氧化物酶(POD)活性的升高。以25μg·ml-1BTH诱导效果最好。     

Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels

The purpose of this study was to investigate the variation of Orf virus (ORFV) immune related genes after infection with different species.The ORFV genomes of sheep and camel were extracted and named ORFV-Y and ORFV-LT,respectively.Based on ORFV genome sequence published in GenBank (accession No.:KF234407.1),three pairs of specific primers were designed and synthesized to amplify the B2L,F1L and VIR gene fragments of ORFV-Y and ORFV-LT,respectively,and the amplified fragments were cloned into pMD19-T vector,transformed into E.coli DH5α competent cells.The recombinant plasmid was identified,and positive clones were selected for sequencing,DNAStar software was used to analyze the homology,amino acid sequence and phylogenetic tree of 13 ORFV genome sequences published on NCBI.The results showed that the nucleotide homology of B2L,F1L and VIR genes were 92.8% to 99.2%,95.7% to 99.5% and 77.6% to 100%,respectively.After comparing the amino acid sequence between the two genomes and the reference sequence,it was found that there were obvious differences in the immune related genes between the two genomes,and F1L gene had some rules to follow.The phylogenetic analysis of B2L,F1L and VIR genes showed that ORFV-Y was closely related to the Chinese Fujian goat strain,while ORFV-LT was far from the reference strains,and was a separate branch.The results showed that ORFV had obvious difference in immune related genes between sheep and camels,it provided a reference basis for further research on the changes of ORFV gene sequences in different species and the development of vaccines for different species in the future.     

苜蓿皂甙对绵羊氮平衡及生产性能的影响

选用12只2周岁体况良好,体重35 kg的内蒙古半细毛羯羊,随机分成4组,每组3头,分别饲喂4种不同水平苜蓿皂甙的日粮(0、8、16、32 g/d)。试验结果表明,在试验期内,苜蓿皂甙对绵羊氮平衡及生产性能有影响。当苜蓿皂甙添加量为8g/d时,氮沉积及日增重最高。