猕猴桃AdCCS1基因的克隆及其表达调控

采用RT-PCR法从‘米良1号’猕猴桃中分离到1 066 bp的铜锌超氧化物歧化酶分子伴侣蛋白基因AdCCS1,登录号为KY471361。AdCCS1的开放阅读框为984 bp,编码327个氨基酸,包含3个典型结构域（N端结构域、中间结构域和C端结构域）和2个保守的金属结合位点（MXCXXC和CXC）。基因结构分析显示AdCCS1由6个外显子和5个内含子组成。系统进化分析表明AdCCS1聚在双子叶植物分支中,与茶树CsCCS的亲缘关系最近。此外,AdCCS1的5′端调控区存在多种光响应、胁迫响应和激素响应应答元件。定量PCR分析显示,AdCCS1在猕猴桃叶片中的表达量最高,其次是成熟果、花、幼果和茎,在根中的表达量最低。猕猴桃果实中AdCCS1在4 ℃低温贮藏过程中的表达量均比25 ℃贮藏中同期的低,说明低温抑制AdCCS1的表达。脱落酸和赤霉素处理后AdCCS1的表达下调,但二者的应答模式不同。     

SIRT1 promotes autophagy of pancreatic cancer cells induced by hypoxia via regulating FOXO1/RAB7 signaling pathway

AIM: To investigate the effect of SIRT1 on the autophagy of pancreatic cancer cells under hypoxia condition, and to analyze the underlying mechanism of regulating FOXO1/RAB7 signaling pathway. METHODS: Western blot and immunofluorescence methods were used to determine the expression of SIRT1 in the pancreatic cancer cells. The small interfering RNA targeting SIRT1 and SIRT1 over-expression plasmid were transfected into the pancreatic cancer Panc-1 cells. Confocal microscopy was used to detect the LC3 expression. Western blot was used to analyze the protein levels of LC3, p62 and FOXO1/RAB7 signaling pathway-related molecules. Co-immunoprecipitation was used to detected the protein interaction between SIRT1 and FOXO1. RESULTS: The expression level of SIRT1 in the nucleus of Panc-1 cells was increased under hypoxia condition. Compared with negative control under hypoxia condition, knock-down of SIRT1 expression attenuated the autophagy flux in the pancreatic cancer Panc-1 cells (P<0.05). Over-expression of SIRT1 increased the protein levels of FOXO1 and RAB7. On the contrary, knock-down of SIRT1 expression inhibited the protein levels of FOXO1 and RAB7. The protein interaction between SIRT1 and FOXO1 in the pancreatic cancer cells was observed. CONCLUSION: SIRT1 in pancreatic cancer Panc-1 cells under hypoxia condition is over-expressed in the nucleus. Down-regulation of SIRT1 inhibits autophagy and its mechanism may be related to FOXO1/RAB7 signaling pathway.     

商陆抗病毒蛋白在植物抗病上的应用

商陆抗病毒蛋白是指商陆属植物中的核糖体失活蛋白.阐述了商陆抗病毒蛋白的研究历史、意义、种类和理化性质,对病原菌的抗性、抗病机理及其在植物抗病上的应用情况等,并对它的应用前景作了展望.     

贮藏时间对马铃薯杂交实生籽发芽率的影响

用2003-2008年这6 a所制的马铃薯杂交实生种子做发芽试验。试验结果表明,刚过休眠期的种子发芽率高,而贮藏时间长或是未过休眠期的种子发芽率低,随贮藏年限的增加,发芽率逐渐降低。     

卷丹LlAGO1基因的克隆及表达分析

以卷丹（Lilium lancifolium）叶腋组织为研究材料,利用RT-PCR和RACE技术克隆得到AGO1基因的cDNA全长,命名为LlAGO1。其全长4 014 bp,开放阅读框长3 687 bp,编码1 228个氨基酸残基,其编码蛋白分子量为135.36 kD,理论等电点（pI）为9.57。氨基酸序列分析表明LlAGO1含有PAZ和Piwi两个AGO1典型的结构域;信号肽预测结果表明LlAGO1蛋白不存在信号肽,为非分泌蛋白;亚细胞定位预测其主要定位于细胞核;与相关同源蛋白高度相似,且与芦笋AGO1a蛋白（XP_020260210.1）亲缘关系最近。qRT-PCR分析结果表明：LlAGO1在卷丹叶腋、鳞片、根、叶等不同组织均有表达,其中在叶腋中的表达量最高,叶片和根中的表达较弱;腋生珠芽形成过程中,LlAGO1仅在可形成珠芽的上部叶腋表达,且在珠芽形成时表达量最高,而在不形成珠芽的下部叶腋几乎不表达,推测LlAGO1可能与卷丹珠芽的形成相关。     

花椰菜新品种雪剑一号的选育

雪剑一号是以福建地方品种经7代连续自交选育出的优良自交系CH19为父本,以雄性不育系02-9-10为母本配制成的早熟秋花椰菜一代杂种,生育期60d左右;植株生长势较强,株型直立紧凑,外叶深绿色、蜡质中等,叶柄较短,内叶合抱自护球;花球雪白、紧实、高圆形,单球质量1.12kg左右,每667m2产量2600kg左右。     

Effect of external counterpulsation on asymmetry diethylarginine and endothelin levels in blood

AIM:To observe changes of serum asymmetry dimethylarginine (ADMA) and endothelin-1 (ET-1) levels,which reflect blood vessel endothelial function,after therapy of enhanced external counterpulsation(EECP) in patients who had taken percutaneous transluminal coronary angioplasty and stent.METHODS:Fifty one coronary heart disease patients (all of them had taken percutaneous tranluminal coronary angioplasty and stent) were distributed into two groups by matching them with ratio of 1∶2,17 patients in EECP group and 34 patients in control group.Both of two groups were given conventional medicine,in addition,EECP group was undertaken three courses of treatment of EECP.ADMA was detected by HPLC-fluorescence method,and ET-1 was detected by radio-immunity method.RESULTS:In EECP group ，compared with prior treatment,ADMA and ET-1 levels was obviously reduced (P<0.05).In control group,compared with prior treatment,ADMA had no significant difference (P>0.05) and ET-1 was higher (P<0.01).Compared between two groups,extent of decrease in ADMA and ET-1 levels in EECP group were more obvious than control group (P<0.01).Improvement of angina and decrease of frequency of angina was more obvious in EECP group than control group.Besides,decrease of ADMA level was positive correlation with them (r=0.85，0.70,respectively P<0.01).CONCLUSION:EECP reduces serum ADMA and ET-1 levels by increasing shear stress to vessel endothelium in coronary heart disease patients who had taken percutaneous tranluminal coronary angioplasty.It hints that EECP can improve endothelial function and provides experimental evidence for the combined treatment of coronary heart disease.     

樱桃番茄新品种钱江红珠的选育

钱江红珠是以X1R为母本、KY-10-1为父本配制而成的杂交1代樱桃番茄新品种.该品种属无限生长型,中熟,生长势强.果实长圆,大红色,大小均匀,单果质量16～18 g,硬度好,耐贮运,春季667 m2平均产量5000 kg左右,抗TMV(Tobacco mosaic virus),中抗CMV(Cucumber mosa...     

广西玉米纹枯病菌培养性状及其致病力研究

从广西玉米主产区的8个县市秋玉米的纹枯病病株中分离出8个玉米纹枯病菌株,经生物性状的观察和菌丝融合群测定表明,它们都属于立枯丝核菌AGk-1-IA融合群;腐生定殖能力和致病力测定表明,菌株GX4、GX1的腐生定殖能力和致病力最强。