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51.
李淑芬 《农业图书情报学刊》2012,24(2):111-112,133
西文配套辅导书著录信息源情况复杂,是西文图书编目的一大难点。通过实例对西文配套书著录中易出现的问题进行探讨,并提出了西文配套书正确著录的方法。 相似文献
52.
云南山区少数民族社区的可持续发展,是云南全面实施可持续发展战略的重要组成部分,是云南区域性可持续发展研究中的前沿性课题。为探求云南山区少数民族社区可持续发展之路,该研究采用了云南省山地民族行政村可持续发展指标体系对云南省永仁县他的苴村1991~2001年可持续发展态势进行评价,结果表明:1991~2001年他的苴村处于基本可持续发展的状态。 相似文献
53.
农牧交错地区沙漠化土地整治与开发利用 总被引:2,自引:0,他引:2
本文从自然条件的特点和生产的实际出发,对农牧交错地区沙漠化土地的整治模式与技术及其开发利用的前景作了较为详细的论述。提出这一地区沙漠化土地的整治必须与开发利用相结合;与农牧林业生产的实际相结合。在整治中开发,在开发中整治,才能达到既整治环境又发展生产的目的。 相似文献
54.
Dowdall SM Proudman CJ Love S Klei TR Matthews JB 《Research in veterinary science》2003,75(3):223-229
Cyathostomins are important equine gastrointestinal parasites. Mass emergence of mucosal stage larvae causes a potentially fatal colitis. Mucosal stages are undetectable non-invasively. An assay that would estimate mucosal larval stage infection would greatly assist in treatment, control and prognosis. Previously, we identified two putative diagnostic antigens (20 and 25 kDa) in somatic larval preparations. Here, we describe their purification and antigen-specific IgG(T) responses to them. Western blots confirmed the purity of the antigens and showed that epitopes in the 20 kDa complex were specific to larval cyathostomins. No cross-reactive antigens appeared to be present in Parascaris equorum or Strongyloides westeri species. Low levels of cross-reactivity were observed in Strongylus edentatus and Strongylus vulgaris species. Use of purified antigens greatly reduced background binding in equine sera. These results indicate that both antigen complexes may be of use in a diagnostic assay. 相似文献
55.
Stefancíková A Stĕpánová G Derdáková M Pet'ko B Kysel'ová J Cigánek J Strojný L Cisláková L Trávnicek M 《Veterinary research communications》2002,26(8):601-611
In the course of epizootological research on Lyme borreliosis in domestic and farm animals, the serological evidence for the occurrence of this zoonosis in cattle was screened. An ELISA showed that 25.2% of cattle from seven geographical areas in Slovakia were positive for anti-Borrelia IgG antibodies. In particular localities, the seroprevalence ranged from 0.6% to 34.3%. Of 33 cases with clinical signs, 20 ELISA-positive samples were also confirmed in Western blots. The most frequent clinical signs were lameness and swollen joints, but most of the cases were asymptomatic. The occurrence of Borrelia burgdorferi antibodies and suspected clinical signs in cattle of Slovak regions indicates that veterinarians should pay attention to this disease in their clinical practice and include it within the differential diagnosis. 相似文献
56.
Atkinson RA Cook RW Reddacliff LA Rothwell J Broady KW Harper P Ellis JT 《Australian veterinary journal》2000,78(4):262-266
OBJECTIVE: To investigate the seroprevalence of Neospora caninum infection in a commercial dairy cattle herd, 15 months after detection of an abortion outbreak. PROCEDURE: Sera from the whole herd (n = 266) were examined for N caninum antibodies by indirect fluorescent antibody test (IFAT) and immunoblot analysis. Herd records were reviewed to collate serological results with abortion history, proximity to calving, and pedigree data. RESULTS: The seroprevalence of N caninum infection was 24% (63/266) for IFAT titre > or = 160, 29% (78/266) for immunoblot positive (+ve), and 31% (82/266) for IFAT > or = 160 and/or immunoblot +ve; 94% (59/63) of animals with IFAT > or = 160 were immunoblot +ve. The association between seropositivity (IFAT > or = 160 and/or immunoblot +ve) and history of abortion was highly significant (P < 0.001); the seroprevalence was 86% (18/21) in aborting cows, compared with 30% (50/164) in non-aborting animals. The abortion rate for seropositive cows was 26% (18/68) compared with 3% (3/117) for seronegative animals. IFAT titres of infected cows were higher within 2 months of calving than at other times (P < 0.001). The association between seropositivity in dams and daughters was highly significant (P = 0.009). CONCLUSIONS: The abortions were associated with N caninum infection and there was evidence of reactivation of latent infection close to calving and congenital transmission of infection. Immunodominant antigens identified by immunoblots may prove useful for improved diagnostic tests. 相似文献
57.
Suksawat J Hegarty BC Breitschwerdt EB 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(1):50-55
Ehrlichia canis, E. equi, and E. risticii seroprevalence was determined by microimmunofluorescent antibody testing (IFA) in a sequential population of 1,845 sick dogs admitted during a 1-year period to the North Carolina State University Veterinary Teaching Hospital. A seroreactor was defined by a reciprocal IFA titer of > or =80 to E. canis, E. equi, or E. risticii antigens. Of the 48 IFA seroreactors, 44 dogs were seroreactive to E. canis, 21 to E. equi, and 0 to E. risticii. Seventeen dogs reacted to both E. canis and E. equi antigens. There was concordance of E. canis IFA and western immunoblot (WI) test results for 36/44 dogs. Because of cross-reactivity of E. canis sera with E. equi antigens, WI was of less utility to confirm E. equi exposure. After elimination of E. canis seroreactors, there was concordance of 2/4 E. equi IFA and WI test results. Based upon a retrospective review of medical records, ehrlichiosis was diagnosed in 10/48 (21%) IFA seroreactive dogs, 9 of which were confirmed positive by WI. Of the remaining 38 IFA seroreactors, 29 also were confirmed by E. canis or E. equi WI. These results indicate that (1) ehrlichiosis was not diagnosed in the majority of serologically confirmed cases, (2) based upon E. canis and E. equi WI analysis, IFA testing was not specific (21% false positive), (3) E. canis sera cross-react with E. equi antigens, and (4) serologic evidence of E. risticii infection was lacking in the dog population studied. 相似文献
58.
59.
将堆型艾美球虫(E.acervulina)3-1E基因克隆至pET-32a(+)载体中。转化大肠杆菌BL21,在37℃下,用终浓度为1.0mmol/L的IPTG诱导表达,得到相对分子质量约为38500的重组蛋白。Western blot检测证实,该重组蛋白可以与特异性抗血清结合。在20℃条件下,以终浓度分别为2.0、1.0、0.5mmol/L的IPTG进行诱导.当IPTG终浓度为0.5mmol/L时,以可溶性形式存在的目的蛋白含量最高。 相似文献
60.
西北牧区灌溉人工草地适宜发展规模分析 总被引:3,自引:0,他引:3
针对西北牧区水资源和草地资源已被超极限利用的现状,应用水草畜平衡原理,分析了西北牧区灌溉人工草地的发展规模,提出了西北牧区灌溉人工草地适宜发展规模为215.76~239.71万hm^2,载畜量控制在13833.18万羊单位之内即可实现草畜平衡,总体可达到人工种植冷季补饲水平。新增灌溉需水量约为71.85~83.55亿m^3,占2000年灌溉总用水量(777亿m^3)的9.25%~10.75%。解决这一灌溉需水量的可行途径是建设节水型社会,提高水资源利用效率。 相似文献