川西茶馆文化的复兴与发展研究

对传统川西茶馆文化进行总结梳理,发现当下的川西茶馆功能日渐萎缩。可通过挖掘传统川西茶馆的历史文化价值,将川西乡村茶馆的发展与新农村建设和社区自治结合起来,将乡村茶馆建设成为代表川西社会独特生活方式和社会主流文化的教育基地,重塑川西茶馆文化。     

自组织视野下西部地区特色城镇化建设的路径选择及其依据

从宇宙世界的本然规律出发,认为西部地区特色城镇化建设路径应以自组织为基础、他组织为促进,走生态城镇化建设道路,并从其理论依据和事实依据进行了论述。     

家蚕脂蛋白基因BmLp-c21的表达分析及蛋白质亚细胞定位

家蚕30 kD脂蛋白家族在家蚕的生长发育过程中具有重要的生理功能。从家蚕蛹cDNA文库中克隆到一条编码30kD蛋白质的基因cDNA序列,该序列全长2 803 bp,ORF为792 bp,编码含263个氨基酸残基的脂蛋白(low molecular 30K lipo-protein pBmHPC-21;GenBank登录号:Q00801),命名为BmLp-c21。将BmLp-c21克隆至原核表达载体pET-28a(+),转化E.coli Rosetta(DE3),IPTG诱导表达后通过亲和层析得到纯化的融合蛋白,并免疫新西兰大白兔制备多克隆抗体。亚细胞定位显示BmLp-c21主要存在于细胞质中,呈点状或者片状分布。通过实时荧光定量PCR和Western blotting方法,分别检测到家蚕不同发育时期和5龄幼虫不同组织中BmLp-c21 mRNA的转录水平与蛋白质的表达水平存在较大差异,在蛹期及5龄幼虫血淋巴中的mRNA转录水平和蛋白质表达水平最高。初步推测BmLp-c21在家蚕蛹的变态发育过程以及蚕体脂质的运输方面具有重要的功能。     

阿替美唑对小型猪特异性麻醉剂诱导大鼠大脑皮质内Fos蛋白表达的影响

研究阿替美唑对小型猪特异性麻醉剂(XFM)麻醉下大鼠大脑皮质Fos蛋白表达的影响,探讨阿替美唑颉颃XFM催醒大鼠与大脑皮质c-fos基因的关系。将72只SD纯种大鼠随机分为XFM对照组、XFM+阿替美唑组、XFM+生理盐水组,每组又按采脑时间点分成4个亚组。采用蛋白质印迹法检测大脑皮质内Fos蛋白表达量。结果表明:XFM麻醉大鼠大脑皮质内Fos蛋白表达量逐渐增加,与给药后10min比较差异显著(P<0.01或P<0.05);XFM麻醉大鼠腹腔注射生理盐水,各时间点Fos蛋白表达量与对照组间比较无显著差异(P>0.05);阿替美唑注射后引起XFM麻醉大鼠大脑皮质Fos蛋白表达量减少,与XFM对照组比较差异显著(P<0.01或P<0.05)。结果提示,大脑皮质c-fos基因参与了阿替美唑颉颃XFM麻醉作用。阿替美唑抑制XFM诱导大鼠大脑皮质Fos蛋白表达,可能是阿替美唑催醒XFM麻醉大鼠的重要机理之一。     

小鼠出生后背部毛囊发育与Lef-1表达的研究

旨在研究Lef-1在小鼠背部毛囊生长周期中的表达规律及其与毛囊生长更替的关系。本试验采用石蜡切片HE染色、Western blotting以及免疫组织化学方法,研究小鼠毛囊的周期性变化,检测Lef-1在小鼠毛囊生长的不同时期皮肤组织中的蛋白表达水平,并对其进行组织定位。结果,昆明系小鼠毛囊生长呈周期性变化,处于不同生长时期的毛囊具有各期的特殊结构;Western blotting结果显示,在各阶段皮肤组织中存在Lef-1蛋白的表达,其在毛囊生长初期的表达量显著高于中期和末期;免疫组织化学结果显示,Lef-1在不同毛囊生长时期的皮脂腺、根鞘和真皮乳头均有不同程度的表达。Lef-1的表达与毛囊的周期性变化存在相关性。     

Non-mycorrhizal fungal endophytes in two orchids of Kaiga forest (Western Ghats), India

We used standard isolation protocols to explore the endophytic fungal communities in three tissue types of two dominant orchids (Bulbophyllum neilgherrense and Vanda testacea) of the Kaiga forest ofthe Western Ghats. We surface sterilized and assessed 90 segments ofeach orchid for the occurrence and diversity of endophytic fungal taxa.The 118 fungal isolates were obtained from root, bulb and leaves of B.neilgherrense, consisting of 17 anamorphic taxa (range, 10 15 taxa) with 1.3 fungal taxa per segment (range, 1.2 1.4 taxa). Four taxa (Aspergillus flavus, A. niger, Penicillium sp. and morpho sp. 1) belonged to the coregroup (11.1% 32.2%). The relative abundance of A. flavus and morpho sp.1 was more than 10%. A total of 130 fungal isolates from roots, stems and leaves of V. testacea yielded 20 anamorphic taxa (range, 11 15 taxa)with 1.4 fungal taxa per segment (range, 1.4 1.5 taxa). Aspergillus flavus,A. niger, A. ochraceus, Gliocladium viride, Penicillium sp. and morphosp. 1 belonged to the core group. Relative abundance exceeded 10% for A. flavus, A. niger, and morpho sp. 1. The Simpson and Shannon diversity indices were higher in leaf than root or bulb/stem of both orchids.Jaccard’s similarity coefficient was higher between root and leaf in both orchids (56.3% 60%) than between other pairs. Our study revealed thatthe endophytic fungal assemblage and diversity of B. neilgherrense and V.testacea of Kaiga forest of the Western Ghats were relatively similar between orchids and their tissues.     

3个杨树品种在鲁西地区光合生理特性研究

在鲁西地区对鲁林2号、鲁林3号、2025杨(对照)3个杨树品种的净光合速率、蒸腾速率、气孔导度等生理指标进行测定的结果表明:鲁林2号、鲁林3号的净光合速率显著高于对照,具有高产潜能。相关性分析发现,蒸腾速率、空气温度与杨树净光合速率分别呈极显著和显著正相关关系,说明杨树要实现高产,必须有充分的水、热条件做保障。     

Anthropogenic Influences on Wild Nutmeg Fruit Crop in the Western Ghats of India

Myristica dactyloides is characteristic of the mid- and high-elevation evergreen forests of the Western Ghats of India. Aril and seeds of the species are valuable nontimber forest products and are harvested extensively. We made visual estimates of fruit crop size in nine populations. We assessed disturbance using five variables: distance from road, method of harvesting fruits, nonforest matrix around the population, seedling regeneration, and protected area status. Unsustainable harvest and proximity to road impacted fruit crop, as did the nonforest matrix (F = 38.17 df = 5,102, p < .001). While high protection and measures against harvesting keep some populations safe, the harvest method plays a crucial role in determining total fruit production and seedling recruitment in the species.     

浅谈西部环境法律体系建设与完善

指出了为适应深入实施西部大开发的发展战略,加强西部地区的生态建设,要求建立与完善与其相适应的环境法律法规。从西部环境保护的法律体系现状出发,分析了现有环保法律体系的不足。同时,从自然和社会两方面提出了完善现在法律体系的措施,并强调了国家机关、社会组织、公民的监督的作用。     

A Framework for Economic Analysis of Cropping Systems: Profitability,Risk Management,and Resource Allocation

SUMMARY

A cropping system must be economically viable for it to be sustainable. This study focuses upon the economic components of profitability, risk management potential, and optimal resource allocation. Some of the primary aspects of economic analysis of cropping systems to a broad multidisciplinary audience of researchers, extension specialists, graduate students, and senior undergraduate students are addressed. Analytical economic tools are discussed including data requirements, advantages, and disadvantages of each method. A case study of a Henderson, Kentucky producer is presented as a primary example along with empirical applications from the literature. The importance of various types of economic analysis is demonstrated through the selection of different production decisions dependent on the level of economic analysis included.