Zerumbone attenuates MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells by inhibition of oxidative stress

AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP⁺)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP⁺-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP⁺ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP⁺ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP⁺-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis.     

Effect of shikonin on high glucose-induced apoptosis and oxidative stress in vascular endothelial cells

AIM:To investigate the effect of shikonin on the apoptosis and oxidative stress induced by high concentration of glucose in vascular endothelial cells. METHODS:Rat thoracic aortic endothelial cells were randomly divided into 5 groups:normal control group (with glucose at concentration of 5.5 mmol/L in cell culture medium), high glucose group (with glucose at concentration of 33 mmol/L in cell culture medium), high glucose+low shikonin group (with glucose at concentration of 33 mmol/L and shikonin at concentration of 0.1 μmol/L in cell culture medium), high glucose+medium shikonin group (with glucose at concentration of 33 mmol/L and shikonin at concentration of 1 μmol/L in cell culture medium), and high glucose+high shikonin group (with glucose at concentration of 33 mmol/L and shikonin at concentration of 10 μmol/L in cell culture medium). After treatments, the cell viability was measured by CCK-8 assay and cell apoptotic rate was analyzed by flow cytometry. In addition, the status of oxidative stress was evaluated by determining the levels of malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). The activation of Nrf2/HO-1 signaling pathway was determined by Western blot. RESULTS:Compared with high glucose group, shikonin reversed high glucose-induced decrease in cell viability and increase in apoptosis in a concentration-dependent manner. High concentration of glucose induced high levels of MDA and ROS, while decreased the levels of SOD and GSH-Px. However, after treatment with shikonin, the contents of MDA and ROS were decreased, while the activities of SOD and GSH-Px were increased as compared with high glucose group. Furthermore, the high concentration of glucose up-regulated the protein levels of cleaved caspase-3, HO-1 and Nrf2 (nuclear). Compared with high glucose group, the protein levels of cleaved caspase-3, HO-1 and Nrf2 (nuclear) were partly decreased after treatment with shikonin. CONCLUSION:Shikonin alleviates high glucose-induced vascular endothelial cell apoptosis. Its mechanism may be related to activation of Nrf2/HO-1 signaling pathway and down-regulation of oxidative stress in vascular endothelial cells.     

几种混凝土衬砌防渗渠的比较与选优

本文在对３种混凝土防渗渠进行定性分析的基础上，利用“模糊综合评判法”进行了综合评价，得出了“大力推广钢模预制Ｕ形渠，逐步淘汰卷板式Ｕ形渠，限制使用梯形渠”的结论。     

基于ARM9内核的智能灌溉控制系统的设计

介绍了基于ARM920T内核的嵌入式控制系统的设计与实施,并将控制系统引入设施栽培自动灌溉控制中。实践证明,该系统的设计达到了预期的效果。     

钻头牙轮三维实体造型的设计

以UG为二次开发平台，采用参数化设计思想，利用UG／OPEN GRIP语言编程，对钻头牙轮三维实体造型进行了研究。利用GRIP语言直接读取牙轮轮廓曲线数据，生成了牙轮三维实体，同时实现了在牙轮布齿锥面上生成齿孔和生成简单牙轮轴承的三维实体造型。通过该软件的应用，可以极大的缩短钻头牙轮的设计周期，提高产品的设计质量。     

基于Pro/E的高度可调式人机学座椅设计

日常生活中随处可见的座椅给人们带来了很大的舒适性,但也出现了因长时间保持坐姿而产生的腰酸背痛情况。原因在于传统座椅的设计较少引入人体因素,因此,只有运用人机学原理设计的新型座椅才能解决这一问题。此外,诸如Pro/E之类的CAD软件也应该在现代设计中得到广泛应用,故本文尝试使用Pro/E建立模型。     

长期服用氨氯地平对高血压病降压的疗效及左室结构功能的影响

目的；观察氨氯地平治疗高血压病的长期降压效应及左室结构功能的改变。方法：２６例患者日服氨氯地平５－１０ｍｇ，平均２４个月。于服药前，服药后４周及治疗结束时进行偶测血压，动态血压及彩色多普勒超声心动图检查。结果：治疗后偶测血压，白昼及夜间动态血压明显平稳下降，心率则差异无统计学意义，室间隔，左室后壁厚度及左室重量指数均显著下降。     

关于非线性方程组求解技术

提出了新的非线性方程解法。在进行结构非线性平衡路径的全过程分析时，在仔细研究了由Ｃｒｉｓｆｉｅｌｄ和Ｒａｍｍ提出，并被广泛用于非线性方程求解的著名的弧长增量法的基础上，提出了一种基于牛顿－拉菲逊法的十分有效的投影增量法，该法克服了弧长增量法的一个重点缺点，即必须根据结构特性来判定如何选取关于广义荷载参数λ＾ｉ＋１的一元二次方程中的二个根中的一个，而且其收敛速度要稍快，计算量也略小。并通过引进广义时     

野生和人工栽培缬草香精油的GC/MS分析对比研究

应用ＧＣ／ＭＳ／ＤＳ分析对比研究野生和人工栽培香料油，分别鉴定出３４个和２８个化合物，两种香精油其相组分２１个。占总精油组分的９１％－９６％，主成分基本相同，占精油总量的７８％－８５％，其成分上的差异对其在香料工业、日化、食品、饮料、医药工业上的应用没有影响，为缬草开发利用建立家种缬草原料基地，保证缬草香料油工业化生产提供依据。     

Biochemical indices (RNA/DNA ratio and protein content) in studying the nutritional status of Ruditapes decussatus (Linnaeus 1758) juveniles

In this work, we investigated the nutritional status of Ruditapes decussatus juveniles under different rearing conditions, using biochemical indices (RNA/DNA ratio and protein content) and the linear instantaneous growth rate (IGR). Two experiments were conducted to study the effect of starvation, addition of substrate (sand) as rearing support and diet composition on somatic growth and biochemical indices. Results show that biochemical indices of fed juveniles were significantly (P<0.05) higher. Highest RNA/DNA values and protein content were recorded in fed juveniles reared with substrate (P<0.05); conversely, lowest values were recorded in starved ones reared without substrate. In the second experiment, the highest RNA/DNA ratio was recorded in juveniles fed with control algal mixture composed of species commonly used in bivalve nurseries [Isochrysis galbana (clone T-Iso), Chaetoceros calcitrans and Tetraselmis suecica] and lowest in those fed with algal species isolated from Tunisian coasts. This confirms the usefulness of the RNA/DNA ratio and protein content in the clam's nutritional status evaluation. This assay is useful to provide information about the nutritional status and health of the clam in field conditions.