松口蘑的驯化栽培及人工促繁

在国家松茸自然保护区建设的基础上 ,应用松茸窝理论阐述松口蘑驯化栽培和人工促繁的基本理论和基本方法。     

谷秆两用稻草粉替代麦麸栽培珍稀食用菌

为了解决食用菌生产中的“菌牧”矛盾，更好地发展食用菌业和畜牧业．我们利用谷秆两用稻草粉替代麦麸作培养料，栽培珍稀食用菌秀珍菇、金福菇、茶薪菇、真姬菇等。结果表明：用谷秆两用水稻“201”优质稻草粉50％代替栽培料中的麦麸栽培秀珍菇、金福菇、茶薪菇，生物效率分别提高了5．84％(差异不显著)、7．98％(差异不显著)和25．16％(差异极显著)；100％代替麦麸栽培真姬菇(占培养料总量的20％)生物效率减少3．68％(差异不显著)。同时降低栽培袋的污染率，降低培养料成本，节省了大量麦麸用作饲料粮，提高了经济效益和社会效益。     

松口蘑 Tm-3 菌株的紫外诱变育种

对松口蘑(Tricholorna matsutake)Tm-3出发菌株进行紫外诱变处理，将茵丝体生长快且茂盛的诱变菌株进行初筛和复筛，比较茵丝体生长量和胞内多糖产量．筛选出诱变株Tm-3-21为胞内多糖发酵的优质菌株，其胞内多糖产量达0．67mg／mL，得率是9．41％，比出发菌株提高1．19倍。     

巨大口蘑子实体营养成分分析

测定了巨大口蘑子实体中氨基酸、矿质元素、多糖、粗蛋白、粗脂肪、灰分和粗纤维含量。结果表明，在测定的18种氨基酸中，8种必须氨基酸含量占氨基酸总量的40．63％；矿质元素K、Na、Ca、Mg、Cu、Fe、Zn、Mn和Si含量分别是17654．35、1644．28、177．96、909．26、184．12、171．54、102．01、3．91和1．28mg／kg；多糖、粗蛋白质、粗脂肪、灰分和粗纤维含量分别是11．59％、36．59％、4．26％、6．31％和9．43％。     

草原蒙古口蘑蘑菇圈的特殊生态现象观察

蒙古口蘑(Tricholoma mongolium Imai.),也称蒙古白蘑,是著名的优质野生食用菌和典型的蘑菇圈产生菌.     

巨菌草饲粮对七彩山鸡血液指标和脂肪相关基因的影响

旨在探究日粮中添加巨菌草对七彩山鸡血液生理生化指标以及脂蛋白脂酶(LPL)基因和钙激活蛋白酶抑制蛋白(CAST)基因表达影响.在为期3周预试验后,选取1周龄(无外观上缺失,无精神、食欲不振等情况)健康的七彩山鸡(母)210只,随机分为1个对照组和4个处理组,每组3个重复,每个重复14只,5个组分别是在基础饲料中添加0％...     

Giant sequoia regeneration in group selection openings in the southern Sierra Nevada

Fire has been linked to the regeneration of giant sequoia (Sequoiadendron giganteum) [Lindley] Buchholz but no studies have directly investigated the effects of opening size and fuel treatment on giant sequoia establishment. Giant sequoia seedling density was analyzed in 36 group selection openings harvested in 1993 at Mountain Home Demonstration State Forest, CA. The experiment consisted of four replicates of a randomized 3 × 3 factorial design which investigated the effects of opening size and fuel treatment on giant sequoia regeneration. Small, medium, and large circular openings had average diameters of 15, 30, and 61 m, respectively. A total of 12 large, 12 medium, and 12 small openings were created. Three fuel treatments were randomly applied to the openings: tractor pile and burn, broadcast burn, and lop and scatter. Analysis of variance (ANOVA) detected no significant differences between treatments and seedling density (p < 0.05). All the treatments had low giant sequoia seedling density regardless of opening size or fuel treatment. Regeneration was completely absent in all openings with the lop and scatter fuel treatment while small openings had low giant sequoia regeneration density regardless of fuel treatment. Giant sequoia seedling density was low because of below average annual and summer precipitation following the creation of the openings and of low seed dispersal. The group selection silvicultural system attempts to simulate the structural complexity of the prehistoric, patchy, high intensity fire regime that once existed in the mixed-conifer forests of the southern Sierra Nevada but important ecosystem processes such as increased seed dispersal following patchy, high intensity fire and large scale nutrient cycling are not duplicated.     

大百合中铜和锰的原子吸收光谱直接测定方法

[目的]建立简便、快速、精确的大百合中高含量铜、锰的测定方法。[方法]采用次灵敏线（铜327．4nm、锰280．1nm）确定大百合中高含量铜、锰的分析条件,建立大百合中高含量铜、锰的次灵敏线原子吸收光谱测定方法,并考察酸及酸含量、共存离子等对测定结果的影响。[结果]含量低于10％的盐酸、硝酸、王水、高氯酸、硫酸对5mg／L铜和锰的测定结果无影响;2000mg／L K＋、Na＋,1000mg／LFe3＋、Zn2＋、mg2＋、Ca2＋100mg／LAl3＋、Si4＋及50mg／LPb2＋、Cd2＋、Cr6＋、Ni2＋均不干扰铜、锰的测定;铜、锰的线性jia范围分别为0-25、0—12mg／L,相对标准偏差分别为0．42％和0．66％,加标回收率分别为96％～103％和97％-102％。[结论]该研究建立的大百合中铜、锰含量的测定方法简便、快速、实用性强。     

长白山区松口蘑组织分离比较试验

本文以松口蘑子实体作为分离材料，对予实体的不同部住应用4种培养基进行组织分离。结果表明：菌褶为最佳分离部位．分离成功率最高时达到100％，山楂-黑木耳菌丝麦麸浸汁培养基为最佳分离培养基。本试验从菌柄基部分离得到2支葡酒色被包霉。     

RAPD标记在松口蘑菌丝体鉴定中的应用

利用筛选的分离培养基从4个长白山松口蘑菌褶中各自分离了菌丝体,应用RAPD标记对其真伪进行了鉴定.结果表明：分离的菌丝体是其来源松口蘑子实体的纯培养物;所选培养基可用于松口蘑菌丝体的分离;长白山松口蘑子实体之间遗传差异较大.