基于遥感的山东省土地利用时空变化和驱动力分析

利用山东省1970s、1980s、1995、2000、2005、2010和2015年共7期土地利用数据,采用土地利用动态度、结构变化、土地利用类型转移矩阵研究人类土地利用活动导致的生态环境变化,结果表明1970s—2015年山东土地利用类型变化较大,除建设用地增加迅速外,耕地、林地、草地和未利用土地均呈不同程度减少趋势...     

表达小反刍兽疫病毒H基因的山羊痘病毒通用转移载体的构建及鉴定(英文)

[Objective] This study was to develop a live vector vaccine of goat pox virus of Peste des petits ruminants(PPR). [Method] Using PCR amplification technique, PPR H gene was obtained, then ligated into pGEM-T easy vector; the recombinants were digested by Nhe Ⅰ and Hind Ⅲ, and ligated into pEGFP-N1-P7.5, yielding the recombinant vector pEGFP-N1-P7.5-H; next the expression cassette EGFP-N1-P7.5-H was first released from recombinant vector pEGFP-N1-P7.5-H by double digestion of Hind Ⅲ and Nhe Ⅰ and ligated into pUC119-TK that was digested by Kpn Ⅰ, yielding the transfer vector pUC119-TK-EGFP-P7.5-H. [Result] Identification and double enzyme digestion showed that the transfer vector pUC119-TK-EGFP-P7.5-H was correctly constructed. From the transfer vector transfected BHK-21 cells which infected GTPV AV41, specific fluorescence was observed at 48th h of transfection. [Conclusion] The construction of goat poxvirus live vector laid a foundation for the live vector vaccine of PPR vaccine.     

波尔山羊胚胎移植产业化关键技术研究

为使波尔山羊胚胎移植形成产业,对供、受体羊的选择、同期发情、超数排卵、采胚、检胚与移植等关键技术进行了研究.在非繁殖季节(北京地区 4月至 8月),供体波尔山羊 15只,受体羊(马头山羊) 76只,受胎率为 71.0%,移植产羔率为 42.1%;在繁殖季节(北京地区 9月至翌年 3月),供体波尔山羊 34只,受体羊(马头山羊) 98只,受胎率为 68.4%,移植产羔率为 48.0%.这表明波尔山羊胚胎移植可以从研究阶段发展到产业化应用阶段.