Yak interferon-alpha loaded solid lipid nanoparticles for controlled release

To explore the potential of a novel animal interferon formulation for controlled release, the yak interferon-alpha (IFN-α) glutathione S-transferase (GST) fusion protein was expressed in Escherichia coli (E. coli) and the purified recombinant IFN-α was encapsulated into solid lipid nanoparticles (SLN) by double emulsion solvent evaporation (w/o/w) method. The particle size and zeta potential of IFN-α-loaded SLN were 124.2 ± 10.2 nm and −11.2 ± 0.6 mV. The encapsulation efficiency of IFN-α and loading capacity of the SLN were 83.7 ± 4.5% and 1.73 ± 0.15%, respectively. In vitro release study and antiviral assay demonstrated that the IFN-α released from the SLN in a 16-day period exhibited antiviral activity in Madin-Darby bovine kidney (MDBK) cells against vesicular stomatitis virus (VSV), and showed a release pattern of an initial burst release followed by a sustained and slow release. Cytotoxicity assay in cell culture demonstrated that the SLN were not toxic. The results of this exploratory study suggest that the IFN-α-loaded SLN could be a useful formulation for controlled release in veterinary therapeutics.     

Analysis of the toxic potential of Palicourea corymbifera (Müll. Arg.) Standl. in laboratory animals

Palicourea species may produce bovine toxicity. Palicourea corymbifera grows in terra firme forests within the Amazon rain forest and in Tropical America, particularly in spots that gave place to gazing areas. The lyophilized extract done with the aerial organs of P. corymbifera were analyzed in male and female mice. Results revealed a significant toxicity: LD50 was 1.10 (1.04-1.15)g/kg for male mice, and 1.05 (1.00-1.10)g/kg for female mice. Locomotion was affected as well as there were reflexes linked to environmental stimuli in addition to changes in posture. Progressive central nervous system stimulus signs such as trembling and convulsions were detected, the latter followed by the animal's death. Macroscopic histopathological exams performed on the liver, kidneys and lungs of mice submitted to necropsy did not indicate the existence of lesions. General activity of animals, measured in an open field, was reduced as a result of the administration of the extract. Duration of locomotion and rearing frequency were reduced, in opposition to an increase in the duration of immobility. Thin layer chromatography analysis showed that monofluoroacetic acid is present in the lyophilized extract, but other qualitative techniques as gas chromatography/mass spectrometry and 19F nuclear magnetic resonance showed that the MFAA was not present in the extract, and that the toxicity is related to other compound, although the toxic profile is very similar to that of MFAA. P. corymbifera was shown to be significantly toxic to laboratory animals and investigation of the possible toxic substance shall be done.     

二甲基甲酰胺对家蚕毒性试验

二甲基甲酰胺对家蚕的毒性较低，只有在很高的浓度食下时才表现出急性中毒死亡，并且随着龄期的增加，家蚕的耐毒性增加，3龄蚕比2龄蚕有更强的耐毒性。低浓度的二甲基甲酰胺（DMF）对家蚕生命率和经济性状都没有显著影响。DMF对家蚕的毒性有积累作用，3龄期连续添毒稀释10倍的DMF原液24h没有观察到急性中毒死亡，但连续添毒90h后，可最终导致家蚕中毒死亡。     

AA肉鸡腿肌重活体估测方法的探讨

以AA肉鸡商品代为试验材料,分析0、7、14、21、28、35、42日龄公、母鸡整体和腿肌的累积生长,建立腿肌重(y)与活重(x)之间的直线回归、幂回归、指数回归、对数回归及多项式回归方程,探讨腿肌重的活体估测方法。结果表明,AA肉鸡的腿肌在后期生长迅速,尤其是公鸡在35～42日龄时期表现突出;腿肌重(y)与活重(x)的幂回归、直线回归、多项式回归方程的拟合精度均在0.95左右,效果较好。     

甘肃陇西黄芩灰霉病病原菌分离鉴定及田间药剂防治

为探明黄芩(Scutellaria baicalensis Georgi)茎基部和根部灰霉病的病原菌,本文于2019—2021年春季对甘肃陇西县采集的黄芩病样进行病原菌的分离培养,运用形态学和内转录间隔区(Internal transcribed spacer, ITS)、甘油醛-3-磷酸脱氢酶(Glyceraldehyde-3-phosphate dehydrogenase,G3PDH)、热激蛋白60(Heat shock protein 60,HSP60)和RNA聚合酶亚基II(The second largest subunit of the nuclear RNA polymerase enzyme II,RPB2)多基因方法鉴定,采用菌丝生长抑制法进行药剂室内毒力测定和田间药剂防治试验。结果表明：引起黄芩灰霉病的病原菌为灰葡萄孢(Botrytis cinerea),春季发病时表现为地上部分枯死不发芽,靠近地表的根表面呈暗绿色水渍状,随后地下根木质部变软呈黄褐色腐烂,病健交界明显;室内毒力测定表明,97%咯菌腈对灰葡萄孢菌的抑菌作用最强,EC₅₀为0.01...     

稻虱净对家蚕毒性和安全性评估

在稻桑混栽区，施用浓度约１０００ｐｐｍ稻虱净时，将被污染的桑叶饲蚕，或在实验室用约１０００ｐｐｍ稻虱净从一龄蚕连续添毒，均能对家蚕造成慢性中毒，发生迟眠蚕，多营下茧和烂茧，全茧量、茧层量显著下降。     

蒲草垫料水浸出物、醇浸出物、醚浸出物的急性毒性试验及小鼠饥饿试验研究

小鼠在正常状况下仅有撕咬垫料行为，在饥饿状态下少量嚼食薄草垫料，但不引起小鼠中毒。急性毒性试验表明，蒲草的水浸出物，醇浸出物，醚浸出物的ＬＤ５０分别大于蒲草２２．４、１５．７、１９．６ｇ／ｋｇ体重，灌胃后小鼠行为、反应正常，无中毒症状。４日后处死剖检结果，脏器亦无病变。大鼠经皮肤涂抹浸出物（每次剂量分别相当于蒲草７６．０、５３．３、６６．７ｍｇ／ｃｍ２，重复３次）后，无全身中毒症状，涂抹部位无临床上肉眼可见病变。兔滴眼试验（每次剂量分别相当于蒲草１２８．５、９０．２、１１２．７ｍｇ／ｋｇ体重，重复３次）证明，眼无临床上肉眼可见病变。大鼠经口及兔经皮试验亦得到相同结果。说明蒲草垫料的三种浸出物对动物无毒害作用。     

Egg production forecasting: Determining efficient modeling approaches

Several mathematical or statistical and artificial intelligence models were developed to compare egg production forecasts in commercial layers. Initial data for these models were collected from a comparative layer trial on commercial strains conducted at the Poultry Research Farms, Auburn University. Simulated data were produced to represent new scenarios by using means and SD of egg production of the 22 commercial strains. From the simulated data, random examples were generated for neural network training and testing for the weekly egg production prediction from wk 22 to 36. Three neural network architectures-back-propagation-3, Ward-5, and the general regression neural network-were compared for their efficiency to forecast egg production, along with other traditional models. The general regression neural network gave the best-fitting line, which almost overlapped with the commercial egg production data, with an R(2) of 0.71. The general regression neural network-predicted curve was compared with original egg production data, the average curves of white-shelled and brown-shelled strains, linear regression predictions, and the Gompertz nonlinear model. The general regression neural network was superior in all these comparisons and may be the model of choice if the initial overprediction is managed efficiently. In general, neural network models are efficient, are easy to use, require fewer data, and are practical under farm management conditions to forecast egg production.     

65%桑用毒死蜱乳油对家蚕的毒性试验

通过2007年春季65％桑用毒死蜱乳油的家蚕毒性饲养试验，发现在1000～4000倍使用浓度范围内，间隔3-5d家蚕表现急性中毒死亡；间隔6-8d家蚕中毒死亡率随浓度增高而增高，随浓度降低，家蚕中毒死亡龄次逐步延后，出现积累性中毒症状，同等有效剂量，桑用乳油的生活力和茧质指标明显好于一般乳油；间隔14～16d后，各处理的生活力和茧质指标与不喷药对照无显著差异存在。65％桑用乳油2000倍喷药，安全用叶间隔期春季推荐为15～20d；秋季为20～25d。     

草原补奖政策下牧户家庭收入的影响研究——以内蒙古锡林郭勒盟为例

为探究草原补奖政策对牧户家庭收入的影响,本研究基于锡林郭勒盟219户牧民调研数据,运用分位数回归模型,对牧民收入的影响因素和作用程度进行分析。结果显示：草原补奖政策促进牧民增收,尤其是对低收入牧户家庭增收效果显著;草场使用面积、年初家畜数量、劳动力比例、家畜出生率及出售率对牧民收入具有显著正影响,当草场使用面积、年初家畜数量分别增加1公顷、1羊单位,人均总收入分别提高0.02%,0.15%;劳动力比例、家畜出生率及出售率分别提高1%时,人均总收入分别提高0.65%,0.43%,1.83%;各因素对不同收入家庭的收入影响及作用程度具有差异性,即随着收入水平的提高,劳动力比例增收效果趋于加强,而家畜出生率和出售率的增收效果则趋于减弱。因此,建议引导牧民发展畜牧业适度规模经营、加大畜牧业社会化服务体系建设等以提高牧民收入水平。