不同海拔高度茶树芽叶主要生化物质含量的变化

本文报导了川西茶区不同海拔高度茶树新梢主要生化成份含量的变化。茶树新梢氨基酸的含量,随海拔高度的不同出现高低的变化,以1100—1200米为最多。咖啡碱的含量相对稳定,与海拔高度没有明显的相关性。茶多酚含量随海拔升高而降低。因此,川西盆边山区发展茶叶生产时,垂直分布高度以不超过1200米为好。     

马立克病毒UL36~（USP）蛋白的表达及其抗体制备

UL36USP是马立克病毒UL36基因编码的蛋白UL36上具有去泛素化酶活性的N-端部分片段（UL36USP）,本试验以提取MDCC-MSB1细胞的基因组为模板通过PCR获得目的基因,将UL36USP基因克隆入pMD18T载体,测序正确并酶切鉴定后,将目的基因亚克隆到pET28a原核表达载体,构建重组质粒pET28a-UL36USP。将重组质粒转化BL21（DE3）E.coli中,IPTG诱导表达并使用Ni-NTA Agarose亲和层析进行纯化得到目的蛋白。应用纯化后的UL36USP免疫獭兔制备多克隆抗体。使用昆虫细胞表达系统表达的UL36片段检测多克隆抗体的特异性。结果显示成功制备特异性的UL36USP抗体。     

牛病毒性腹泻病毒E0基因重组表达质粒pMG36e-E0的构建与免疫原性分析

利用SacⅠ、HindⅢ限制性内切酶分别对pMG36e与pMD19-T-E0进行双酶切,将目的基因E0整合入穿梭表达载体pMG36e,构建牛病毒性腹泻病毒E0基因重组表达质粒pMG36e-E0。提取重组质粒pMG36e-E0,进行双酶切鉴定及PCR鉴定。将阳性重组质粒pMG36e-E0转化到大肠杆菌DH5α中进行表达,对表达产物进行SDSPAGE和Western-blotting鉴定。用表达的E0蛋白二次免疫家兔,间接ELISA法检测其血清抗体水平。结果,重组质粒经双酶切得到大小分别约为3 600、691bp的2个片段,PCR扩增出691bp的片段,双酶切与PCR鉴定结果表明目的基因E0正确插入到载体pMG36e中。SDS-PAGE电泳结果表明E0基因在大肠杆菌获得了表达,表达产物相对分子质量大小约为27 000。Western-blotting分析结果证明表达产物能被BVDV阳性血清所识别。ELISA检测结果证明表达的E0融合蛋白能刺激动物产生抗体,具有天然蛋白的免疫原性。     

Reproduction related immunoglobulin changes in rainbow trout

Annual changes in plasma immunoglobulin (IgM) levels were investigated in three strains of rainbow trout Oncorhynchus mykiss which have different spawning periods, i.e., September–October, November–December, and January, reared under constant water temperature and natural day length. Plasma IgM levels decreased during the spawning season in all strains tested. The IgM changes became reversed in response to significant increases in plasma testosterone (T) and estradiol-17 in females and T and 11-ketotestosterone in males. Though the IgM decline showed a connection with suppressed immunocompetence, since many mature fish caught fungal diseases, no clear differences were observed in the plasma IgM levels between infected and noninfected fish during the spawning season. Incidentally, plasma IgM levels in infection prone fish were higher than in noninfection prone fish prior to the spawning season, whereas coincident differences in the plasma steroid levels were observed. Immature fish reared under lower water temperatures showed lower IgM levels. The effect of water temperature may have to be considered when analyzing the defense mechanism during the spawning season in rainbow trout.     

大豆新品种晋豆36的选育及高产栽培技术

晋豆36是山西省农科院小麦研究所与临汾市尧都区种子公司合作育成,简要介绍晋豆36特征特性、产量表现及栽培技术.     

水貂阿留申病发病机理的研究进展

水貂阿留申病是由阿留申病病毒引起的一种可对水貂养殖业造成严重损失的传染病。该病困扰动物医学界多年 ,始终未得到攻克。近年来随着分子生物学技术的发展 ,国内外学者对该病分子水平的发病机理有了更多的认识 ,研究内容主要集中在与水貂阿留申病发病密切相关的抗病毒抗体、病毒核酸的晚启动子P3 6及其顺式作用元件(cis acting)、结构蛋白VP2等方面。文章对以上研究进展进行了详细的归纳总结与分析 ,并在此基础上提出了尝试使用反义RNA或干扰RNA对该病进行预防、治疗的设想 ,以期今后对阿留申病的继续深入研究能有所帮助。     

SQR基因在大肠杆菌和乳酸菌中的表达

为了研究硫化物-醌氧化还原酶(SQR)基因的乳酸菌表达载体pMG36e-SQR-Myc的构建及其在大肠杆菌DH5a和乳酸菌MG1363中的表达,通过限制性内切酶酶切大肠杆菌-乳酸菌穿梭表达载体pMG36e与目的片段SQR-Myc,回收纯化并用T4连接酶进行连接,连接产物通过KCM法转化到大肠杆菌DH5a中,提取所得到阳性大肠杆菌菌株质粒pMG36e-SQR-Myc进行双酶切鉴定与普通PCR鉴定;并通过电转化将重组质粒导入乳酸菌MG1363中,采用SDS-PAGE电泳和Western-blotting检测SQR蛋白质在其中的表达情况。结果显示,SQR乳酸菌重组表达载体pMG36e-SQR-Myc被成功构建,目的蛋白质SQR在大肠杆菌和乳酸菌MG1363中被成功检测到。因此,乳酸菌MG1363可以用来表达硫化物-醌氧化还原酶(SQR)。     

杜氏利什曼原虫NH36基因的克隆及其在大肠杆菌中的表达

应用RT—PCR技术扩增并克隆杜氏利什曼原虫（Leishmania donovani）NH36编码基因，经鉴定及序列分析，构建其原核重组表达载体，经IPTG诱导表达，SDS-PAGE和Western-blotting鉴定其表达产物。结果显示，获得的NH36开放阅读框为945bp，编码314个氨基酸残基，与GenBank上发表的国际标准株NH36编码基因序列以及氨基酸序列同源性分别为88．57％（837／945）和89．17％（280／314）。表达蛋白为36ku，经1mol／L IPTG诱导6h后的表达量最高；薄层扫描显示表达的蛋白占菌体蛋白总量的57％；该蛋白可被抗杜氏利什曼原虫的多克隆抗体血清识别。     

Expression of SR-A II and CD36 in white blood cells correlate with plasma AGEs levels and diabetic complications

AIM: To investigate the correlation of the expression of scavenger receptors SR-A II and CD36 in white blood cells (WBCs) with the plasma levels of advanced glycosylation end products (AGEs) and different diabetic complications.METHODS: Blood samples were collected from 78 patients with diabetic complications. The levels of plasma AGEs were determined by using spectrofluorimetry. RNA in WBCs was extracted with Trizol reagent and the mRNA levels of SR-A II and CD36 were determined by RT-PCR. RESULTS: In the patients tested, the mRNA level of SR-A Ⅱ was found to be the highest in those with diabetic nephropathy, and lowest in those with fatty liver. The expression of CD36 was found to be the highest in diabetic patients with fatty liver and lowest in those with coronary heart disease. The expression of both receptors in WBCs showed significantly higher levels in diabetic patients with hypertension, and lower in those with cataract. The plasma levels of AGEs negatively correlated with mRNA levels of CD36 (r=-0.89,P<0.01), while positively correlated with SR-A II mRNA levels (r=-0.82, P< 0.05).CONCLUSION: The increased plasma levels of AGEs may stimulate the expression of SR-A II in WBCs, and higher expression of SR-A Ⅱ and CD36 was significantly related to diabetic complications, nephropathy and fatty liver, respectively. However, low expression of CD36 in some diabetic patients with complications might be important causes for their high plasma AGEs levels.     

水稻抗性品种IR36甲醇提取物不同极性组分对褐飞虱的活性

研究了抗褐飞虱Nilaparvata lugens(Stl)水稻品种IR36甲醇提取物各种不同极性组分对褐飞虱生物型Ⅱ成虫单向选择性、产卵拒避率、取食量和若虫生长发育等影响。结果表明,各种组分对褐飞虱的定向拒避率为51.59%~97.64%,产卵拒避率为48.91%~86.90%;与对照相比,取食量减少2.5~5倍,虫体重量增加率降低约6~12倍,若虫的死亡率增高2~6倍,羽化率降低1.5~5倍,以上各项指标与对照相比均达到差异显著水平。说明各种组分对褐飞虱生物型Ⅱ成虫定向反应和产卵选择有拒避作用,极性越低的组分作用越大;对褐飞虱取食行为有抑制作用,对若虫生长发育表现为负作用,导致其死亡率增加和羽化率降低,极性越强的组分作用越大。