CO 2与土壤水分交互作用的番茄光合速率预测模型

为了实现不同土壤水分管理下的CO 2气肥精细控制,建立了番茄作物不同生长阶段的光合速率预测模型。实验设置了4个CO 2浓度与3个土壤水分条件的交互处理,利用无线传感器网络长期实时监测温室内环境信息,采用LI-6400XT型光合速率仪定时采集作物净光合速率信息;并用BP神经网络分别建立了番茄苗期、花期和果期的光合速率预测模型。预测模型的验证结果表明,对于苗期预测模型,预测值与实测值之间的决定系数 R 2为0.925;花期预测模型的决定系数 R 2为0.920,果期预测模型的决定系数 R 2为0.958;番茄各生长期的光合速率预测模型均具有较高的预测精度。在不同土壤水分条件下改变CO 2浓度,得到的CO 2浓度与光合速率预测曲线与实测值相近,可反映实际土壤水分管理下的CO 2浓度最优值,对指导不同土壤水分条件下CO 2气肥的精细调控具有重要意义。     

Preliminary Study on the Activity of Phycobiliproteins against Botrytis cinerea

Phycobiliproteins (PBPs) are proteins of cyanobacteria and some algae such as rhodophytes. They have antimicrobial, antiviral, antitumor, antioxidative, and anti-inflammatory activity at the human level, but there is a lack of knowledge on their antifungal activity against plant pathogens. We studied the activity of PBPs extracted from Arthrospira platensis and Hydropuntia cornea against Botrytis cinerea, one of the most important worldwide plant-pathogenic fungi. PBPs were characterized by using FT-IR and FT-Raman in order to investigate their structures. Their spectra differed in the relative composition in the amide bands, which were particularly strong in A. platensis. PBP activity was tested on tomato fruits against gray mold disease, fungal growth, and spore germination at different concentrations (0.3, 0.6, 1.2, 2.4, and 4.8 mg/mL). Both PBPs reduced fruit gray mold disease. A linear dose–response relationship was observed for both PBPs against disease incidence and H. cornea against disease severity. Pathogen mycelial growth and spore germination were reduced significantly by both PBPs. In conclusion, PBPs have the potential for being also considered as natural compounds for the control of fungal plant pathogens in sustainable agriculture.     

间作番茄对甘蓝生长及其主要害虫种群数量的影响

研究了甘蓝、番茄不同间作比例对甘蓝生长及主要害虫种群数量的影响,以期找到适宜的间作比例,为生产实践中进一步推广应用提供理论依据。试验结果表明,甘蓝与番茄间作模式,对甘蓝主要害虫菜青虫、小菜蛾、菜蚜等有一定的趋避效果,但6∶1的间作处理效果较差,4∶1、2∶1的间作处理效果良好,且差异不明显;2∶1的间作处理由于间作密度小,甘蓝受番茄生长的影响大,植株生长不良,6∶1的间作处理由于间作密度较大,甘蓝受虫害影响大,叶球虫孔数多,商品性差,4∶1的间作处理,植株生长良好,叶球饱满、质量大、商品性好。综合各项数据分析,4∶1的间作比例更合适。     

亚精胺浸种对番茄种子萌发及幼苗高温抗性的影响

以番茄品种合作903为试验材料,设置0、0.1、0.2、0.4 mmol · L~(-1 )亚精胺(Spd)浸种10 h,研究不同浓度Spd浸种对番茄种子萌发、幼苗生长及高温抗性的影响。结果表明:不同浓度Spd浸种处理均能显著提高番茄种子的发芽指数和活力指数,幼苗地下部干质量和壮苗指数明显提高,其中0.2 mmol · L~(-1 )Spd浸种处理发芽最快,活力指数最高。Spd浸种处理降低了高温胁迫下番茄叶片的相对电解质渗透率、丙二醛(MDA)和H_2O_2含量,其中0.2 mmol · L~(-1 )Spd处理的相对电解质渗透率、MDA和H_2O_2含量最低;抗氧化酶(SOD、APX、DHAR)活性增加,并在0.2 mmol · L~(-1)时达到最大值。综上,0.2 mmol · L~(-1 )Spd浸种能有效促进番茄种子萌发和幼苗生长,提高番茄幼苗高温抗性。     

小麦秸秆复合基质对番茄幼苗生长发育的影响

为了充分利用农业废弃物小麦秸秆,进行草炭替代性育苗基质原料的研发,实现农业资源循环化利用和生态可持续发展。以番茄‘毛粉802’为材料,通过小麦秸秆腐熟物、草炭、蛭石和珍珠岩4种基质原料及1种生物菌肥进行不同体积配比处理,研究不同配比的小麦秸秆复合基质对番茄幼苗生长指标、叶片色素及荧光指标、幼苗叶片理化性质指标、基质生物指标和理化性质的影响,探究适合番茄幼苗生长的小麦秸秆复合育苗基质配方。利用灰色关联法对19项指标进行综合分析。结果表明,V腐熟小麦秸秆∶V蛭石∶V珍珠岩∶V生物菌肥=6∶2.5∶1∶0.5番茄幼苗表现最好,而番茄幼苗在纯小麦秸秆腐熟物上的生长量远远低于其他处理,番茄幼苗的表现最差。     

9种杀虫剂对番茄潜叶蛾的防治效果评价

【目的】 筛选应急防控番茄潜叶蛾的绿色、高效农药,为防止进一步扩散为害提供指导。【方法】 选择9种内吸性杀虫剂,分2个浓度梯度,总计在田间设置18个药剂处理和1个清水对照,在药后1、3、7和14 d调查虫口,计算虫口减退率及防效,筛选绿色高效的农药及最佳药剂浓度。【结果】 60 mL /667m²的6%阿维·氯苯酰SC、42 mL /667m²24%甲氧虫酰肼SC、7.5 mL/667m²的20%氯虫苯甲酰胺SC 3种药剂对番茄潜叶蛾的防治效果和虫口减退率均在90%以上,后两者在后期效果最为明显,具有持效性。3种药剂在药后14 d防效分别为93.6%、93.16%和93.72%,虫口减退率分别为91.20%,90.59%和91.36%。37.5 mL /667m²的0.3%印楝素EW在整个过程防效最低,药后14 d防效为38.04%,其减退率仅为14.79%。【结论】 轮换使用60 mL/667m²的阿维·氯苯酰SC、42 mL /667m² 24%甲氧虫酰肼SC、7.5 mL /667m²的20%氯虫苯甲酰胺SC在生产上。     

几种番茄病毒病的症状识别特征与防治策略

本文简述了番茄斑萎病毒病、番茄黄化曲叶病毒病、番茄褪绿病毒病、番茄花叶型病毒病、番茄蕨叶型病毒病5种主要番茄病毒病的症状识别特征。提出加强植物检疫、选用抗病品种、诱虫板与防虫网联用、切断人为传播和昆虫传播途径、调整定植期、清洁田园环境、合理选择杀虫剂进行杀虫控病等防治策略和防治措施,为番茄产业绿色发展提供了建议和参考。     

百香果番茄复合果酒的工艺研究

以百香果和番茄为主要原料,对百香果番茄复合果酒的加工工艺进行了研究。结果表明,最佳的工艺参数为：百香果番茄汁液(V:V)1:2、初始糖度23.0 °Bx、酵母添加量0.25%、发酵温度22 ℃。这四种因素对百香果番茄复合果酒影响的主次顺序为百香果番茄汁V:V >酵母添加量>发酵温度>初始糖度。百香果番茄复合果酒的成品指标为：酒度10.4 °(V%),残糖4.9%,pH值为4.3,呈柠檬黄色,清澈透明无悬浮物、果香酒香浓郁、丰满爽口有新鲜感。     

Discrimination of ‘San Marzano’ accessions: A comparison of minisatellite, CAPS and SSR markers in relation to morphological traits

‘San Marzano’ (SM) is one of the most widely known tomato (Solanum lycopersicum L.) cultivars, and is a classic example of a local variety with a premium value. Unfortunately, the original cultivated form is underrepresented in the Protected Denomination of Origin (PDO) area because of the incidence of contaminant and phenotypically similar genotypes. Our aim was to examine the ability of three DNA marker systems (minisatellite, cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR)) to reveal the genetic diversity of tomato accessions that were, based on a morphological analysis, very similar. The data indicate that both minisatellites and SSRs can be used to genetically distinguish the analysed materials. Furthermore, these two marker systems depict relationships consistent with the hierarchal pattern obtained by the morphological data. As locally cultivated tomato accessions are often characterised by some degree of genetic variability, our results will be valuable in facilitating the purification, management and breeding of tomato germplasms. The differences between the marker systems employed are also discussed in relation to their usefulness in the agro-food chain.     

Resistance of 57 greenhouse-grown accessions of Lycopersicon esculentum and three cultivars to Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

The aim of this study was to evaluate resistance to Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) by antixenosis on 57 Lycopersicon esculentum Mill. accessions from the Horticultural Germplasm Bank (HGB) of Universidade Federal de Viçosa and by the three commercial cultivars (Santa Clara, Moneymaker and TOM-601) under greenhouse conditions. A randomized complete block design was used with three replications. Infestations with T. absoluta adults were performed weekly and the following characteristics were evaluated: number of small, large and total mines/leaf and % of leaves mined at days 60, 75 and 90 after planting. Low infestation occurred at days 60 and 75 but at day 90, infestation was sufficient to evaluate insect damage. Based on these data it was concluded that only accessions HGB-674 and HGB-1497 appeared to be the most promising. In addition, to determine possible chemical causes of resistance, hexane extracts were analyzed at day 90 by gas chromatography/mass spectrometry and the major peaks identified by a mass spectral database using similarity index. Nine hydrocarbons, viz., hexadecane, heptadecane, eicosane, tricosane, 2-methyltricosane, tetracosane, hexacosane, octacosane and triacontane were identified in the hexane extracts in many samples. Tricosane, tetracosane and hexacosane presented significant correlations with the leaves mined. Only tricosane presented a negative correlation with the number of small mines (r = −0.28), total number of mines (r = −0.27) and % of leaves mined (r = −0.22). However, tetracosane and hexacosane presented significant positive correlations (r = 0.25 and 0.24, respectively) with the % of leaves mined.