印度芥菜BjJ10-2基因原核表达载体构建及抗盐功能的鉴定

利用RT-PCR技术克隆了印度芥菜核糖体相关膜蛋白RAMP4家族成员BjJ10-2基因的开放读码框,成功构建了pET32a-BjJ10-2原核表达栽体,通过热激法将其转化到原核表达菌株BL21(DE3)plysS中,得到重组大肠杆菌BL21(pET32a-BjJ10-2).SDS-PAGE凝胶电泳检测到在IPTG诱导下...     

Si4432无线芯片在普适农业系统中的应用

为了解决无线模块发射距离近、穿透能力差的问题,将Si4432模块应用于普适农业系统[1],实现了较大范围农田的温湿度远程监测.该系统由控制中心和传感器节点组成,每个节点包括PIC16F877A单片机、Si4432无线模块和SHT10温湿度传感器,控制中心负责接收、显示、存储和发送温湿度信息.实验证明,该系统可以达到较远的通信距离和较强的穿墙能力,并且通信误码率低,具有很好的推广前景.     

鹌鹑β-防御素10基因的克隆与表达及其体内分布的检测

为测定鹌鹑β-防御素10 (AvBD10)体外抗菌活性及检测其在鹌鹑脏器组织的分布,本研究应用RT-PCR方法从鹌鹑肺脏组织中扩增到鹌鹑AvBD10,经序列同源性分析,鹌鹑AvBD10与鸡AvBD10的氨基酸序列同源性最高(83.8％).将该基因亚克隆到表达载体pGEX-6p-1中进行原核表达,SDS-PAGE电泳表明,鹌鹑AvBD10重组蛋白分子量约为31ku.该重组蛋白经纯化后测定其体外抗菌活性,结果显示,鹌鹑AvBD10重组蛋白具有广谱的抗菌活性.采用定量RT-PCR法检测到AvBD10在鹌鹑脏器组织中广泛表达.     

Hydraulic characteristics of composted pig manure,perlite, and mixtures of them,and their impact on cucumber grown on bags

Composted pig manure (CPM), unmixed or in mixture with perlite, was evaluated as growing medium based on both physical characteristics and agronomic performance. The physical properties were determined in CPM, perlite (0.5–2.5 mm), and their 75:25, 50:50 and 25:75 mixtures by volume. It was found that unmixed CPM has a higher bulk density (BD) than perlite, a slightly lower air space at a suction of 10 cm, and a markedly higher content in easily available water (EAW), although the water content at a suction of 10 cm is the same in the two media. Mixing CPM with perlite by 50:50 seems to increase the actual water content and decrease the actual air content at container capacity, while the BD and the EAW score intermediate to those in the unmixed constituents. Simulation of the relative hydraulic conductivity (K_r) indicates a sharp decrease in the unsaturated hydraulic conductivity (K) of CPM, perlite and their mixtures as the media suction increases. The differences in the decrease rate of K_r between the tested substrates are rather small, with the most and least steep decrease being observed in perlite and the mixture by 50:50, respectively. The agronomic performance of CPM was assessed by growing greenhouse cucumber in bags filled with perlite up to 20 cm in height, CPM up to 10 or 20 cm in height, and their mixture by 50:50 up to the same heights as CPM. A media height of 10 cm in the bag resulted in appreciably higher yields than a height of 20 cm, regardless of the type of the substrate, while the latter had no significant effect on yield. This effect of media height was ascribed to the steep decrease in K, which was minimized in all media at higher suction levels than 10 cm, thereby severely restricting water availability in their upper layers (10–20 cm) when their height in the bag was 20 cm.     

早熟番茄新品种郑番06-10的选育

郑番06-10是以04商引常规-1-混-混为母本,以05早粉父本-混为父本配制的自封顶番茄一代杂种。幼果微具青果肩,成熟果亮粉红色,近圆形,平均单果质量153 g;田间表现对叶霉病、病毒病抗性与中杂9号相当;畸形果和裂果率为2.22 %,抗裂果;果实硬,耐贮运,整齐度高。可溶性固形物含量4.8 %,果肉VC含量294 mg?kg-1,总酸含量0.42 %,可溶性总糖含量2.58 %,品质佳。综合性状表现优良。产量6 400 kg?（667 m2）-1。适合河南、河北、陕西等地早春温室、拱棚、春露地及高山越夏种植。     

Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

Selected wild Dahlia species in their natural habitats from west‐central Mexico were tested for the presence of three caulimoviruses known to be associated with cultivated dahlia (Dahlia variabilis), viz. Dahlia mosaic virus (DMV), DMV‐D10 and Dahlia common mosaic virus. Virus species‐specific primers and PCR were used followed by cloning and sequencing of the amplicons. Results showed that the wild dahlia species in their natural habitat contained DMV‐D10, which is an endogenous plant pararetrovirus. Viral sequences were found in 91% of the samples (n = 56) representing four different wild species. The gene coding for the movement protein of DMV‐D10 from Dahlia coccinea and all other species was cloned and sequenced. Sequence comparisons showed divergence of this gene when compared to that of DMV‐D10 from cultivated dahlias. The discovery of plant pararetroviruses in wild dahlia species in their natural habitats suggests a possible emergence, co‐existence and co‐evolution of pararetroviruses and their host plants.     

10%双烷基二甲基氯化铵水剂防治番茄晚疫病的效果

采用10%双烷基二甲基氯化铵水剂防治日光温室番茄晚疫病,田间试验结果表明:10%DDAC(C8~C10)水剂对番茄晚疫病具有较好的防治效果;其中10%DDAC(C8~C10)水剂1 000倍液6、00倍液的防治效果分别为76.48%、88.04%;10%DDAC(C8~C10)水剂600倍液的防治效果与标准对照药剂69%安克锰锌WP 600倍液的防治效果(90.02%)无明显差异。     

重组结核分枝杆菌CFP10蛋白对A549细胞TLR信号途径介导的炎症反应的影响

本研究旨在检测重组结核分枝杆菌10 ku培养滤液蛋白（culture filtrate protein 10,CFP10）对肺泡Ⅱ型上皮细胞系A549细胞Toll样受体（TLRs）信号途径及其介导的炎症反应的影响。PCR扩增cfp10目的基因片段,构建重组质粒载体pCzn1-CFP10。将重组质粒pCzn1-CFP10转入BL21（DE3）大肠杆菌感受态细胞,IPTG诱导表达重组蛋白CFP10（rCFP10）并鉴定,纯化rCFP10,去除内毒素及脱盐备用。设置对照组,利用MTT检测rCFP10对A549细胞的存活率的影响;通过qRT-PCR、Western blot及ELISA等技术,分别在转录和翻译水平检测rCFP10对A549细胞TLRs信号途径关键分子及其下游炎症因子表达的影响。结果显示,成功构建重组表达载体pCzn1-CFP10并表达纯化出高纯度的rCFP10。MTT结果显示,随着rCFP10浓度增加和处理时间延长,A549细胞存活率显著降低。与空白对照组相比,rCFP10分别从转录和翻译水平显著（P<0.05）或极显著（P<0.01）上调A549细胞中TLRs途径关键分子TLR2、TLR4、MyD88、TRAF6和NF-κB p65及下游炎症因子IL-6、TNF-α的表达水平。rCFP10蛋白可以通过激活A549细胞TLRs受体信号途径促进细胞炎症因子的分泌,这将为进一步加深理解结核病发病机制提供理论依据。     

红壤侵蚀区马尾松林恢复过程中土壤异养呼吸及微生物多样性的变化特征

土壤异养呼吸是影响土壤有机碳积累的关键因素。以南方红壤水土流失区不同恢复年限的马尾松林（未治理地（Y0）、恢复14 a（Y14）、恢复31 a（Y31））为对象,对不同呼吸组分进行测定并结合温度、水分以及微生物等因子,研究马尾松林恢复对土壤异养呼吸的影响。结果表明：不同恢复年限马尾松林土壤异养呼吸差异显著,恢复31 a显著大于恢复14 a以及未治理地,未治理地异养呼吸速率仅为0.99 μmol?m-2?s-1,而治理14 a、31 a分别为2.20、2.80 μmol?m-2?s-1;温度是异养呼吸季节变化的主要影响因子,分别解释季节变化的40.6%（Y0）、62.2%（Y14）、66.6%（Y31）;马尾松林恢复后土壤异养呼吸温度敏感性（Q10）显著增加,Y0、Y14、Y31的 Q10分别为1.58、1.93和1.82;不同恢复年限土壤异养呼吸占土壤总呼吸比例为77.94%（Y0）、70.84%（Y14）、77.35%（Y31）。结构方程表明,在马尾松林恢复过程中,土壤有机碳（SOC）、温度以及土壤微生物多样性变化是影响土壤异养呼吸变化的主要因子,其中SOC、土壤微生物与异养呼吸显著正相关,而植被恢复过程中土壤温度变化与异养呼吸显著负相关。本研究结果表明,马尾松林植被恢复过程中SOC的积累以及缺乏有效的物理保护增加了微生物对SOC的分解,另一方面土壤环境温度的降低和细菌、真菌丰度的增加以及群落中变形菌、子囊菌、酸杆菌的增加,更进一步加剧微生物对原有土壤有机质的分解强度,导致异养呼吸碳排放的持续增加,最终限制了马尾松林土壤碳吸存效率。因此,较高的土壤异养呼吸可能是影响红壤侵蚀退化区土壤有机质进一步提升的关键。