10%氯噻啉可湿性粉剂防治萝卜蚜虫田间药效试验

2006年在重庆万州进行10%氯噻啉可湿性粉剂防治萝卜蚜虫田间药效试验,结果表明,在萝卜蚜虫发生期,用10%氯噻啉可湿性粉剂100、200、300g/hm2剂量喷雾防治,药后1d的平均防效分别为90.7%、97.7%、99.0%,药后7d的平均防效分别为84.0%、95.6%、97.5%,均优于对照药剂25%乐.氰乳油400mL/hm2处理的防效,持效期达7d以上。从防治效果及经济成本考虑,建议10%氯噻啉可湿性粉剂防治萝卜蚜虫以施药量100～200g/hm2、喷液量600kg/hm2为宜。     

新世纪首都水源区发展战略研究报告

作为首都郊区的密云县 ,应该自觉找准本县在首都经济中的战略地位 ,找到一条持续快速健康发展的道路。新世纪首都水源区的发展战略应该是 :着眼首都未来发展对水源和环境的高标准要求 ,切实履行高质量保护水源和环境的特殊职责 ;走环境立县、引进强县、科教兴县和依法治县之路 ;努力建设清洁优美的自然环境、先进完善的设施环境、高效规范的体制环境和健康和谐的人文环境 ;构建以高新技术工业、绿色农业、生态旅游业和环保节能型建筑建材房地产业为支柱的首都水源区经济体系 ;突出县工业开发区、密云卫星城与中心镇建设、旅游西线开发和畜牧业产业化四个战略重点 ,促进经济持续快速健康发展 ,实现强县富民的目标。     

Cracking the Cytotoxicity Code: Apoptotic Induction of 10-Acetylirciformonin B is Mediated through ROS Generation and Mitochondrial Dysfunction

A marine furanoterpenoid derivative, 10-acetylirciformonin B (10AB), was found to inhibit the proliferation of leukemia, hepatoma, and colon cancer cell lines, with selective and significant potency against leukemia cells. It induced DNA damage and apoptosis in leukemia HL 60 cells. To fully understand the mechanism behind the 10AB apoptotic induction against HL 60 cells, we extended our previous findings and further explored the precise molecular targets of 10AB. We found that the use of 10AB increased apoptosis by 8.9%–87.6% and caused disruption of mitochondrial membrane potential (MMP) by 15.2%–95.2% in a dose-dependent manner, as demonstrated by annexin-V/PI and JC-1 staining assays, respectively. Moreover, our findings indicated that the pretreatment of HL 60 cells with N-acetyl-l-cysteine (NAC), a reactive oxygen species (ROS) scavenger, diminished MMP disruption and apoptosis induced by 10AB, suggesting that ROS overproduction plays a crucial rule in the cytotoxic activity of 10AB. The results of a cell-free system assay indicated that 10AB could act as a topoisomerase catalytic inhibitor through the inhibition of topoisomerase IIα. On the protein level, the expression of the anti-apoptotic proteins Bcl-xL and Bcl-2, caspase inhibitors XIAP and survivin, as well as hexokinase II were inhibited by the use of 10AB. On the other hand, the expression of the pro-apoptotic protein Bax was increased after 10AB treatment. Taken together, our results suggest that 10AB-induced apoptosis is mediated through the overproduction of ROS and the disruption of mitochondrial metabolism.     

湄潭县2020年辣椒密度试验

湄潭辣椒种植历史悠久,常年种植面积13 333 hm²,尤其以圆形椒（泡椒品种）种植为特色,为了稳定湄潭黔北优质泡椒基地的地位,加快辣椒产业化建设步伐,特引进黔辣10号（圆形椒杂交种）优质品种,并研究不同密度下其生育性状、经济性状、产量性状的变化。结果表明,黔辣10号种植密度在3 000株/667 m²时,单株结果数47个,产量为1 765.35 kg/667 m²,表现良好。建议黔北海拔700～900 m区域,黔辣10号种植密度为3 000株/667 m²。     

两个红梨品种花色苷合成相关基因及转录因子MYB10表达模式分析

为研究不同红色梨品种着色差异的原因,以西洋梨品种红星和砂梨品种满天红为试材,通过荧光定量PCR方法,分析果皮中花色苷合成基因PAL、CHS、CHI、F3H、DFR、ANS、F3GT和转录因子MYB10基因在果实不同发育期的转录特性。结果表明,在红星中除PAL外,花色苷合成基因表达量与果皮花色苷含量变化一致,先升高后降低;在满天红中,PAL和F3GT在果实转色期表达量最高,其他基因表达量随果实发育而下降。转录因子MYB10在红星整个果实发育期表达水平都很低,在满天红转色期呈现峰值,且表达量是红星的50倍以上。红星着色程度可能由多个基因协同作用,而满天红着色可能受关键基因F3GT的影响,MYB10可能通过调控F3GT的表达从而调控满天红的着色。     

Effects of warming, wetting and nitrogen addition on substrate-induced respiration and temperature sensitivity of heterotrophic respiration in a temperate forest soil

Soil heterotrophic respiration and its temperature sensitivity are affected by various climatic and environmental factors.However,little is known about the combined effects of concurrent climatic and environmental changes,such as climatic warming,changing precipitation regimes,and increasing nitrogen(N)deposition.Therefore,in this study,we investigated the individual and combined effects of warming,wetting,and N addition on soil heterotrophic respiration and temperature sensitivity.We incubated soils collected from a temperate forest in South Korea for 60 d at two temperature levels(15 and 20℃,representing the annual mean temperature of the study site and 5℃warming,respectively),three moisture levels(10%,28%,and 50%water-filled pore space(WFPS),representing dry,moist,and wet conditions,respectively),and two N levels(without N and with N addition equivalent to 50 kg N ha^-1year^-1).On day 30,soils were distributed across five different temperatures(10,15,20,25,and 30℃)for 24 h to determine short-term changes in temperature sensitivity(Q₁₀,change in respiration with 10℃increase in temperature)of soil heterotrophic respiration.After completing the incubation on day 60,we measured substrate-induced respiration(SIR)by adding six labile substrates to the three types of treatments.Wetting treatment(increase from 28%to 50%WFPS)reduced SIR by 40.8%(3.77 to 2.23μg CO₂-C g^-1h^-1),but warming(increase from 15 to 20℃)and N addition increased SIR by 47.7%(3.77 to 5.57μg CO₂-C g^-1h^-1)and 42.0%(3.77 to 5.35μg CO₂-C g^-1h^-1),respectively.A combination of any two treatments did not affect SIR,but the combination of three treatments reduced SIR by 42.4%(3.70 to 2.20μg CO₂-C g^-1h^-1).Wetting treatment increased Q₁₀by 25.0%(2.4 to 3.0).However,warming and N addition reduced Q₁₀by 37.5%(2.4 to 1.5)and 16.7%(2.4 to 2.0),respectively.Warming coupled with wetting did not significantly change Q₁₀,while warming coupled with N addition reduced Q₁₀by 33.3%(2.4 to 1.6).The combination of three treatments increased Q₁₀by 12.5%(2.4 to 2.7).Our results demonstrated that among the three factors,soil moisture is the most important one controlling SIR and Q₁₀.The results suggest that the effect of warming on SIR and Q₁₀can be modified significantly by rainfall variability and elevated N availability.Therefore,this study emphasizes that concurrent climatic and environmental changes,such as increasing rainfall variability and N deposition,should be considered when predicting changes induced by warming in soil respiration and its temperature sensitivity.     

亚麻新品种双亚十号的综合分析与生产应用效果

双亚十号亚麻新品种是黑龙江省科学院亚麻综合利用研究所采用复合杂交方法。经混合个体选择育成,具有高产、高纤、优质、抗病性、抗倒伏性强等优点。2004-2007年累积种植面积达到8．0万hm^2,增产亚麻原茎6万吨,增加经济效益1．8亿元。双亚十号亚麻新品种不仅适宜在黑龙江省亚麻种植区种植,还推广到云南、新疆、内蒙等省区,增产效果显著。     

呼市PM10污染特征分析及预警模型的建立

分析了呼和浩特市可吸入颗粒物PM10污染特征,以及PM10变化规律与气象要素的关系。采用CART方法对造成呼和浩特市中度以上污染的气象条件进行分析,建立了预警模型,确定了呼和浩特市重污染预警指标。     

大肠杆菌Top10Na~+/H~+反向运输体A基因的克隆及生物信息学分析

根据细菌中存在的Na+/H+反向运输体(Na+/H+antiporterA,nhaA)的基因序列,采用PCR扩增的方法从大肠杆菌(Escherichia coli)Top10中克隆到了nhaA基因(Acession Numeber:EU368045)。nhaA开放阅读框为1 167 bp,编码含有388个氨基酸残基,分子量为41.3 kDa的蛋白,预测等电点为9.23。nhaA含有25个碱性氨基酸,19个酸性氨基酸,211个疏水氨基酸及63个极性氨基酸。二级结构预测表明,该蛋白含约50%的α-螺旋、18%的延伸链、7%的β-转角和25%的不规则卷曲。亲疏水性分析显示,nhaA是疏水性蛋白。跨膜结构进行分析显示该蛋白含有11个跨膜区域。序列分析表明,大肠杆菌Top10 nhaA基因与大肠杆菌DH5α、大肠杆菌HS、大肠杆菌SECEC SMS-3-5和大肠杆菌CFT073的nhaA基因同源性分别为100%、98%、95%和92%。大肠杆菌Top10 nhaA基因的克隆及生物信息学分析为今后对nhaA的进一步深入研究奠定了基础。     

Comparison of predictions of baking volume using large deformation rheological properties

Three large deformation rheological tests, the Kieffer dough extensibility system, the D/R dough inflation system and the 2 g mixograph test, were carried out on doughs made from a large number of winter wheat lines and cultivars grown in Poland. These lines and cultivars represented a broad spread in baking performance in order to assess their suitability as predictors of baking volume. The parameters most closely associated with baking volume were strain hardening index, bubble failure strain, and mixograph bandwidth at 10 min. Simple correlations with baking volume indicate that bubble failure strain and strain hardening index give the highest correlations, whilst the use of best subsets regression, which selects the best combination of parameters, gave increased correlations with R²=0.865 for dough inflation parameters, R²=0.842 for Kieffer parameters and R²=0.760 for mixograph parameters.