DETECTION OF PORTAL BLOOD FLOW USING PER-RECTAL 99mTc-PERTECHNETATE SCINTIGRAPHY IN NORMAL CATS

This study reports data obtained from per-rectal ^99mTc-pertechnetate portal scintigraphy in normal cats. It examines the effects of chemical restraint and the methods employed in defining regions of interest (ROIs) on the shunt index derived from this data. Six normal cats were used for the study; all six were chemically restrained for imaging using propofol and later four of them were manually restrained for comparison. Portal blood flow was studied and the mean shunt index was found to be 5.9%± 3.9 when ROIs were operator defined and 9.2%± 4.4 when ROIs were defined using an isocontour program. In cats that were restrained using propofol and operator defined ROIs, the mean value for the time between detection of radioactivity in the liver and in the heart was 14 ± 1 seconds.     

Comparison of Blood Physiological and Biochemical Parameters of Two Kinds of Minipigs in Different Altitudes

This study was designed to compare the blood physiological and biochemical parameters between plateau Juema minipigs and plain Bama minipigs,and provide a theoretical basis for Juema minipigs served as experimental animals in the future.The blood samples of Juema minipigs were taken in Gannan,and the blood samples of the Bama minipigs were taken in Xi'an,24 blood physiological and biochemical parameters were tested using an automatic analyzer of blood cells and biochemistry.Between the plateau Juema minipigs and Bama minipigs,there were significant differences in PLT,RDW-CV and RDW-SD (P< 0.05) and extremely significant differences in RBC,HGB,HCT,MCH,MCMH,GGT,GLU and CRE (P< 0.01).The plateau Juema minipig had an altitude acclimatization,so it was better than Bama minipig in plateau medical science.     

神经元特异性烯醇化酶在大鼠动情周期子宫中的分布

选择健康、未产、体重220-250g成年雌性SD大鼠，采用阴道涂片方法鉴定其动情周期，并采用免疫组织化学SP法研究了神经元特异性烯醇化酶（NSE）在大鼠动情周期子宫内分布的变化规律。结果显示，子宫各层均有不同程度NSE免疫阳性产物分布，并随动情周期的变化表现出一定的规律：各期子宫内膜黏膜上皮细胞、腺上皮细胞、基质细胞、血管内皮细胞及肥大细胞等NSE免疫阳性细胞数量变化不大，但着色深浅有一定差别，动情期着色最深，动情后期、动情间期、动情前期着色依次减弱；肌层和子宫外膜中，动情期着色最深，动情后期着色最浅，动情间期表达量明显回升，动情前期比动情期着色稍浅。表明，NSE在子宫中的分布可能受性类固醇激素的调节。     

抓捕应激对鸡血清酶活性的影响研究

鸡受到抓捕应激后,开始血液中SOD活性降低,到第三天明显低于对照组;随着抓捕应激次数的增多,SOD活性又迅速升高,第十二天显著高于对照组,第九天、第十五天分别极显著高于对照组。CPK活性都高于对照组,而且随着应激次数的增加,CPK活性有逐渐上升的趋势。但抓捕应激对血清中GOT、GPT、APK、LDH、GSH-Px等酶活性的影响不显著。     

杆菌肽锌对夏季产蛋鸡生产性能及血液指标的影响

３８０日龄北京白鸡８０只被随机分成２个处理，每个处理４个重复，每个重复１０只鸡，对照组喂基础日粮，试验组在基础日粮中添加５０ｍｇ／ｋｇ杆菌肽锌．试验被安排在１９９８年６月３０日至７月３０日进行．试验结果表明：杆菌肽锌对产蛋鸡平均产蛋量、平均蛋重及饲料转化率没有显著影响，但却可以明显改善蛋壳质量（蛋壳强度、蛋壳厚度）（Ｐ＜０．０５），并显著减少破软蛋率（Ｐ＜０．０１）．而且在夏季（３２℃以上）蛋鸡产蛋率下降阶段可以减少产蛋率的下降幅度．杆菌肽锌对蛋鸡血液中Ｃａ、Ｐ、Ｚｎ及ＡＬＫＰ（碱性磷酸酶）含量的影响不显著．     

茶多酚、茶多糖对兔动脉血压的影响

兔静脉注10mg／mL的茶多酚和茶多糖溶液，观察对动脉血压的影响，结果表明：注射荼多酚1—3mL后收缩压6次降低（9％-20％），2次升高（13％和20％），3次无变化；舒张压2次升高（20％和140％），9次无变化；注射茶多糖1-4mL后收缩压9次降低（2％-29％），3次升高（13％-40％），5次无变化；舒张压4次降低（10％-200％），7次升高（20％-50％），6次无变化。上述浓度和剂量的茶多酚、茶多糖对兔动脉血压均无显著影响（P〉0．05—0．5）。     

3种提取基因组DNA方法的比较分析

分别采用3种不同的方法提取基因组DNA，比较所提取的DNA的质量以及提取所需时间、费用，以期选择一种高效、简洁快速、价格合理的提取方法。结果发现这3种方法提取的质量都能达到相关分子生物学试验的要求，但在操作步骤、时间、价格等方面存在差异，具体比较结果可为分子生物学试验选择提取方法提供依据。     

Locomotion Score and Its Impacts on Production Performance and Blood Routine Indexes in Chinese Holstein

To investigate the influencing factors of locomotion score (LS) and its impacts on production performance and blood routine index in Holstein,body condition score (BCS),LS and DHI (dairy herd improvement) of 1 486 lactating cows from two dairy farms,and the blood routine indexes of 101 lactating cows from one farm in Beijing were measured.The fixed model was used to analyze the impacts of herd,parity and lactation stage on LS,and the impacts of LS and BCS on daily milk yield,somatic cell score,milk composition and blood routine indexes in dairy cows.The results showed that both parity and lactation stage had significant impacts on LS.The LS increased with the increase of parit,the LS increased by 0.71 from first lactation to fifth and above lactation.The LS of cow in lactation stage Ⅲ(100-199 d) and Ⅵ (after 350 d) were significantly higher than cow in stage Ⅱ (45-99 d)(P<0.05),and both were higher than 2.The LS had no significant impacts on daily milk yield,milk composition and somatic cell score (P>0.05).However,the milk yield of the cow with LS≥4 was the lowest,and a decrease of 2.29 kg was found compared with the cow with LS=1.Among the various blood routine indexes,LS had significant impacts on red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count (P<0.05),and had no significant impacts on white blood cell count (P>0.05).With the increase of LS,the red blood cell count,hemoglobin content,mean corpuscular hemoglobin concentration and lymphocyte count of cows decreased with different extents.There were some problems on the body immunity and hematopoiesis of cows with poor LS.In dairy farm,regular evaluation of LS could be used to find the management problems of feet and legs,which had a great significance to reduce the loss of production performance and early culling caused by hoof disease.     

滴灌马铃薯生长及产量的影响

通过对不同灌水量及不同灌水间隔时间的马铃薯生长状况进行大田滴灌试验,研究马铃薯适宜的灌水定额及灌水周期,以求达到节水、增产的目的。试验结果表明:马铃薯滴灌时,灌水量越大,灌水周期越短,株高越高;茎粗开始衰减的时间比株高要早十几天,且衰减幅度比株高要大。灌水周期最短的处理茎粗却并不是最大,在180 m3/hm2的灌水定额下,灌水周期为5天的处理茎粗值最大。在相同的灌水量下,灌水间隔时间越短的处理马铃薯淀粉含量越高。灌水定额越小,灌水周期对产量的影响就越大;而当灌水定额一定时,灌水周期越短,产量越高;灌水定额为90 m3/hm2,灌水周期为3天的处理产量最高,为28 260 kg/hm2,比7天1灌的处理每公顷产量高出达10 350 kg。