Effect of uric acid on the maturation and the biological function of BMDCs

AIM: To investigate the effect of uric acid on the maturation and the biological function of murine bone-marrow derived dendritic cells (BMDCs) in vitro.METHODS: BMDCs were cultured with GM-CSF, IL-4, LPS and uric acid. Cell phenotypes were analyzed by flow cytometry. MTT was used to detect the effect of uric acid on the function of BMDCs in stimulating the proliferation of T cells. IL-12 released by BMDCs was also detected. RESULTS: BMDCs were cultured and identified. Uric acid at concentrations of 200 mg/L and 400 mg/L increased the expression of the molecules (CD11c, CD83, CD86, IA/IE) on BMDCs surface and the IL-12 level in the culture supernatants (P<0.05), promoted the proliferation of T cells at the T: DC rate 5∶1, 10∶1, 20∶1 (P<0.05). However, uric acid at concentration of 70 mg/L had no effect on above molecule expression (P>0.05), no effect on T cell proliferation with BMDCs (P>0.05) was observed. CONCLUSION: Uric acid promotes the differentiation, maturation, the expression of co-stimulatory molecules, the IL-12 production in BMDCs and enhances the ability of BMDCs to stimulate the proliferation of T cell in a specical dose range.     

刚果12号桉与厚荚相思混交林的养分效应

刚果12号桉与厚荚相思行状混交抑制了两试验树种的生长,混交提高了桉树N营养水平,却降低了桉树P、K的吸收量.混交对林木生长的影响可能由两树种间N的再分配和P、K的竞争的养分效应所致.     

LY12合金冷拉棒性能的研究

对淬火前的热挤压棒用不同的冷拉拔量进行拉拔，结果表明；冷拉拔量对棒材的组织和性能有不同的影响．当变形量达到10％时，经淬火后的棒材其组织是均匀的细晶组织．     

蔗糖和B_(12)对昆明地区油橄榄扦插生根的影响

在昆明市海口林场种植的油橄榄树干较易萌芽,芽条生长快,但是由于管理粗放,插穗营养不良,扦插难生根。因此,从油橄榄喜光、耐高温且插穗营养不良的角度出发,在高温高湿条件下,针对蔗糖、B12及插床设计对油橄榄扦插生根的影响进行试验研究。结果表明,插穗经采用100 mg/l IBA+5%蔗糖+10 ml B12浸泡2 h处理生根率可达69.3%,平均不定根数可达10.9条,平均不定根长4.2 cm,根系效果指数为0.60。插床空间越小,生根效果越好。     

可燃气体检测与报警装置设计

文章介绍了以STC12C5201AD为核心的可燃气体检测与报警装置设计.该装置用催化型可燃气体传感器将气体浓度转换为对应的电压值,然后采用A/D进行采集和标度变换,最后由单片机进行信息处理,判断浓度是否超标,并控制报警动作.     

RF-IC卡门禁系统设计

门禁系统能够给各种需要一定程度管理的场所带来极大的方便.它不仅能制止非法人员的侵入,也可对合法人员的行为时间进行记录,在很大程度上提高了其在安全管理方面的层次,具有较高的现实意义和市场需求.文章主要介绍了RF_IC卡门禁系统,并对系统的总体设计方案作了简要论述.     

基于MC9S12DP256的发动机转速测量系统研究

设计了一种基于飞思卡尔MC9S12DP256单片机的发动机转速测量系统,给出了测量系统的实现技术和硬件结构,进行了软件程序设计,并讨论了转速测量数据的滤波方法。该系统在实际测量中取得了良好的效果,为发动机动力性能研究提供了重要的参考依据,有较强的实用性。     

Molecular phylogeny of the apterygotan insects based on nuclear and mitochondrial genes

Basal hexapodan orders (Apterygota) are traditionally divided in two well defined taxonomic groups, Entognatha and Ectognatha. Entognathy (the enclosed mouthparts condition) is considered the most distinctive character joining the Ellipura (Protura+ Collembola) with the order Diplura, whereas the presence of exposed mouthparts (ectognathy) is the feature shared by Microcoryphia and Zygentoma with the pterygote insects. In spite of the growing interest for the evolutionary history of the Apterygota, there is no complete agreement among the general phylogenetic hypotheses based on the study of morphological characters. In this study we analyzed the DNA sequence of segments of the nuclear Elongation Factor-1α(EF-1α) and of the mitochondrial 12S rRNA genes, and used different methods of phylogenetic reconstruction. The 12S gene seems to be more suited than the EF-1α to resolve some of the most outstanding systematic disputes, whereas the lack of resolution at the deeper nodes does not allow to assess the phylogenetic relationships within Microcoryphia and between ectognathan orders. We have obtained a fairly high support for the monophyly of the orders Diplura and Zygentoma. In the 12S analysis, the Ellipura and the Entognatha form monophyletic assemblages. In addition, the study of the distribution of introns in the EF-1α suggests a relationship between Collembola and Diplura.     

中棉所12(Gossypium hirsutum C.V.Zhongmian 12)体细胞胚发生及植株再生

通过对不同外植体的研究,发现幼胚下胚轴是理想的外植体材料,２,４－Ｄ和ＺＴ是中棉所１２体细胞胚发生的关键植物生长调节物。从愈伤组织诱导到大量再生植株只需４个月左右的时间。