Natural hybridization between wheat (Triticum aestivum L.) and its wild relatives Aegilops geniculata Roth and Aegilops triuncialis L.

BACKGROUND

Cultivated bread wheat (Triticum aestivum L.) spontaneously hybridizes with wild/weedy related Aegilops populations, but little is known about the actual rates at which this hybridization occurs under field conditions. It is very important to provide reliable empirical data on this phenomenon in order to assess the potential crop–wild introgression, especially in the context of conducting risk assessments for the commercialization of genetically modified (GM) wheat, as gene flow from wheat to Aegilops species could transfer into the wild species genes coding for traits such as resistance to herbicides, insects, diseases or environmental stresses.

RESULTS

The spontaneous hybridization rates between wheat and A. geniculata and A. triuncialis, which are very abundant in the Mediterranean area, have been estimated for the first time in the northern part of the Meseta Central, the great central plateau which includes the largest area of wheat cultivation in Spain. Hybridization rates averaged 0.12% and 0.008% for A. geniculata and A. triuncialis, respectively. Hybrids were found in 26% of A. geniculata and 5% of A. triuncialis populations, at rates that can be ≤3.6% for A. geniculata and 0.24% for A. triuncialis.

CONCLUSION

The detection of Aegilops spp.–wheat hybrids in Aegilops populations indicates that gene flow can occur, although wheat is considered a crop with a low-to-medium risk for transgene escape. These data on field hybridization rates are essential for GM wheat risk assessment purposes. © 2023 Society of Chemical Industry.     

Proportionality principle revisited – relationship between application rates and pesticide residue concentrations in food commodities

BACKGROUND

The proportionality principle has been broadly used for over 10 years in regulatory assessments of pesticide residues. It allows extrapolation of supervised field trial data conducted at lower or higher application rates compared to the use pattern under evaluation by adjustment of measured concentrations, assuming direct proportionality between the rates applied and the resulting residues. This work revisits the principle idea by using supervised residue trials sets conducted under identical conditions but with deviating application rates. Four different statistical methods were used to investigate the relationship between application rates and residue concentrations and to draw conclusions on the statistical significance of the direct proportionality assumed.

RESULTS

Based on over 5000 individual trial results, the assumption of direct proportionality was not confirmed to be statistically significant (P > 0.05) using three models: direct comparison of application rates and residue concentrations ratios and two linear log-log regression models correlating application rate and residue concentration or only residue concentrations per se. In addition, a fourth model analysed deviations between expected concentrations following direct proportional adjustment and measured residue values from corresponding field trials. In 56% of all cases, the deviation was larger than ±25%, which represents the tolerance usually accepted for the selection of supervised field trials in regulatory assessments.

CONCLUSION

Overall, the assumption of direct proportionality between application rates and resulting residue concentrations of pesticides was not statistically significant. Although the proportionality approach is highly pragmatic in regulatory practice, its use should be considered carefully on a case-by-case basis. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

吡蚜酮在马铃薯中的残留与膳食风险评估

为评价马铃薯中吡蚜酮残留引发的膳食风险。2016年-2018年开展了3年12地规范残留试验,构建吡蚜酮在马铃薯中的残留检测方法,并对我国一般人群进行了膳食风险评估。马铃薯样品使用2%乙酸乙腈提取,C18净化,外标法定量,UPLC-MS/MS检测。在0.01～1 mg/kg添加水平下,吡蚜酮在马铃薯中的平均回收率为75%～85%,相对标准偏差为3%～8%。12地最终残留试验表明,50%吡蚜酮水分散粒剂按有效成分225 g/hm²施药2次,最后1次施药后14、21 d马铃薯中吡蚜酮残留量分别≤0.018 mg/kg和≤0.012 mg/kg。膳食风险评估表明,吡蚜酮的国家估算每日摄入量(NEDI)为1.633 9 mg/kg,风险商(RQ)为86.4%。马铃薯中吡蚜酮残留不会对一般人群健康造成不可接受的风险。     

Hepatitis E virus in Cuba: A cross-sectional serological and virological study in pigs and people occupationally exposed to pigs

Surveillance of hepatitis E virus (HEV) in risk groups is an important strategy to monitor its circulation pattern and to timely detect changes thereof. The aims of this cross-sectional study were to estimate the prevalence of HEV infections in pigs and humans from different regions of the country, to identify risk factors for increasing anti-HEV IgG prevalence and to characterize HEV strains. The presence of anti-HEV antibodies was assessed by commercial ELISA in serum samples from the general population, farm and slaughterhouse employees, as well as pigs sampled in the three regions of Cuba from February to September 2016. Overall, individuals with occupational exposure to swine or swine products (70/248, 28.2%) were 4 times more likely to be seropositive compared to the general population (25/285, 8.7%; OR: 4.18; p < .001). Within the risk group, risk factors included age, number of years working in a professional activity with direct exposure to swine, geographic region and distance between residence and closest professional swine setting, while wearing gloves had a protective effect. Prevalence of total anti-HEV antibodies in swine was 88.2% (165/187) and HEV RNA was detected by real-time RT-PCR in 9.2% (16/173) swine stools. All HEV strains sequenced clustered within genotype 3. Some strains clearly belonged to subtype 3a, while another group of strains was related with subtypes 3b and 3 k but partial HEV sequences did not allow unequivocal subtype assignment. These findings suggest that the high HEV exposure in Cuban individuals with swine-related occupations could be due to enzootic HEV in certain regions, direct contact with infectious animals or their products as well as environmental contamination.     

基于土地利用/覆盖的甘肃省景观生态风险时空变化特征

甘肃省景观类型丰富多样,在中国西部地区的生态安全中起关键性作用,也为当地发展提供着重要的物质基础和生态服务。由于气候变化和人类不合理活动的影响,甘肃省部分地区生态环境的安全与稳定遭到严峻挑战。本研究基于欧洲航空局全球土地利用/覆盖数据（CCI-LC）,利用动态度、转移矩阵方法分析了2005-2019年甘肃省土地利用/覆盖变化特征;通过构建景观生态风险指数并运用空间自相关分析法分析了景观生态风险的空间分布特征。结果显示：1)2005-2019年间,裸地面积减少最多,变化率1.88%,耕地次之,变化率2.31%;草地面积增加最多,变化率1.83%,建设用地次之,变化率191.08%。2）所有土地利用/覆盖类型中,建设用地的动态度最高且在整个研究时期内变化速率都保持在较高水平,综合动态度最大的时期为2010-2019年。3）土地利用/覆盖转移矩阵主要表现为耕地、林地、草地、裸地之间的转入与转出以及其他土地类型向建设用地的转入。4)2019年整体的生态风险值较2005年明显上升。其中低风险区和较低风险区面积最多,转化最频繁;中等风险区、较高风险区和高风险区面积虽然较少,但都存在不同程度的增长。...     

A Caculation Method of Portfolio Investment under the Risk Mininiz

According to the security investment theory of Markowitz,this paper presents aCaculation method of portfolio investment under the risk minimization,and some mathematic expres-sions of the efficient frontier for portfolio investment are conducted.Based on these,the efficientfrontier is proved as a parabola,and a practical caculation process is given.     

Conditional Value-at-Risk and Its Use in Credit Risk Measurement of Banks

Credit risk, the major risk of bank, is more and more intense during the period of economic restructuring in China. In view of the flaw of present risk measurement system, Conditional Value-at-Risk is used for the credit risk measurement which is better than Value-at-Risk. This paper creates the model ,while gives the method and procedure for solving it. So CVaRof credit combination is produced, which is just the early warning value of credit risk. At last, it is concluded that the CVaRrisk measurement is too difficult to use widely at present in china, then some advice is provided.     

Risk Decision-making Method of Slope Resistant Engineering

Considering the character of slope resistant engineering invest benefit, in line with security and economy balmaced, combining initial investment and anticipated hazard loss, disaster resistant engineering should be satisfied with the criterion of minimum cost. Decision tree model is adopted as analyzing means, and risk decision-making of slope design is carried out. Project input-output radio adopting dynamic analysis can reflect project investment benefit more accurately. At last this paper analyzes some questions in process of slope resistant engineering with benefits enumerate.