布朗李果实耐藏性与简易气调贮藏试验

通过测定果实硬度、固酸比、褐变率、气体变化等分析布朗李不同品种的耐藏性.同时设计包装、乙烯脱除剂等不同处理组合探讨简易气调贮藏布朗李的效果.结果表明,黑宝石、威克森在2个月的贮期内,其硬度、固酸比、褐变率变化均小于黑琥珀、圣玫瑰、玫瑰皇后.在包装与气体处理组合中,硅窗袋、乙烯脱除剂组合能明显地抑制黑宝石布朗李品质指标的下降,使O2浓度明显低于对照,并稳定在0.5%左右,释放的乙烯浓度也较低,起到明显的气调贮藏作用.     

TA29-Barnase 转基因雄性不育芥菜的获得

将烟草TA29 花药绒毡层特异启动子驱动下的核糖核酸酶Barnase 基因,利用农杆菌介导的
方法导入渝丰榨菜幼苗下胚轴中,获得了彻底败育的雄性不育植株。细胞学观察结果显示：转基因芥菜植
株的花药绒毡层细胞提前降解,花粉母细胞退化,成熟花药中无正常花粉粒产生。转基因芥菜植株除了花
药瘦小、开花后无花粉散出、自交不能正常结籽外,还出现了花朵变小、雌蕊变短、种荚变短、结籽量下
降的现象。     

农电66kV变电所继电保护中易忽视的问题

TA饱和影响供电可靠性,严重威胁运行设备的安全。通过介绍农网变电所中10kV线路TA饱和的二次电流特性,以及TA饱和对感应型、电磁型和数字式电流继电器的影响,提出增大保护级TA的变比、减小电流互感器的二次负载以避免TA饱和的方法。同时,分析了励磁涌流对继电保护装置的影响,并提出了解决方法。     

Photosynthetic reaction,mineral uptake,and fruit quality of strawberry affected by different levels of macronutrients

This experiment was conducted to evaluate the effects of three nutrient recipes containing different concentrations of macronutrients in the vegetative stage [Vegetative Nutrient Solution: (VNS-I, VNS-II, and VNS-III)] and two nutrient recipes in reproductive stage [Reproductive Nutrient Solution: (RNS-I and RNS-II)], on physiological and biochemical parameters of strawberry ‘Paros’ in a soilless system. The results indicated the significant effects of nutrient solution on the photosynthetic capacity, yield, minerals uptake and titratable acidity of strawberry fruits. In the other hand fruit total soluble solids affected by vegetative nutrient solution. The highest yield was obtained under application of lowest level of minerals. Vegetative growth including leaf number and leaf area, as well as total chlorophyll were the highest as potassium (K⁺), calcium (Ca²⁺), and nitrogen (N) concentrations were increased. The highest content of N and Ca²⁺ uptake were in VNS-III × RNS-I formulation. Additionally, the highest vitamin C was in VNS-I formulation. Moreover, the most firmed fruits and the highest post-harvest shelf life of fruits were produced in VNS-III × RNS-I formulation.     

Changes in organic acids and acid metabolism enzymes in melon fruit during development

A novel melon cultivar (‘Flavor No. 3’) containing high acid concentration as well as high sugar content in the mature fruit was successfully bred out. The aim of this study is to determine the main organic acids present in this novel melon and to clarify the mechanisms of acid accumulation. Fruit development and acid accumulation patterns in ‘Flavor No. 3’ were compared with those of a conventional low-acid melon, ‘Xuelihong’. The titratable acidity (TA) and organic acids in fruits were measured at different developmental stages. There is a positive correlation between TA and citric acid content in ‘Flavor No. 3’, indicating that citric acid is the predominant organic acid present throughout the fruit development. The activities of the enzymes involved in citric acid metabolism, including phosphoenolpyruvate carboxylase (PEPC), citrate synthase (CS), aconitase (ACO), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), and malic enzyme (ME), were determined. Although no single enzyme was responsible for citric acid accumulation throughout the fruit development, the activities of ACO and ME were positively correlated with citric acid concentration in the two cultivars from 5 to 25 days after pollination (DAP). Moreover, ACO and ME activities in ‘Flavor No. 3’ increased over time and were higher than those in ‘Xuelihong’, resulting in citric acid accumulation in the fruits of ‘Flavor No. 3’. Although a sharp decrease in ACO activity took place after 25 DAP in the fruits of ‘Flavor No. 3’, the earlier accumulation of citric acid was sufficient enough to maintain the high acid concentration until the melon fruits became mature.     

4115TA1型发动机节能改造试验分析

对4115TA1型发动机的部分节能改造措施进行了正交试验，应用综合平衡法确定了对节能效果影响因素的主次顺序是喷油器的型式（B）、活塞环位置（D）、涡流室型式（C）及发动机转速（A）。     

优质酿酒杨梅选种指标体系的研究

对浙江仙居地区优质酿酒杨梅选种指标体系的研究表明 ,固酸比 (可溶性固形物 /总酸 )是优质酿酒杨梅选种的一级指标 ,果实的总酸是二级指标 ,成熟时果实的颜色是三级指标 ,此外 ,在颜色满足酿酒需要的条件下 ,成熟时果实的大小也是一个重要的选种指标。荸荠种为该地区最佳酿酒品种 ,其次是东魁、临海早大梅 ,水杨梅较差     

运动控制技术在根系监测系统中的应用

分析了根系监测系统的组成,采用步进电机作为执行元件组成运动控制系统,并分析了步进电机驱动电路的构成及细分驱动的方法。利用ATmega16单片机和TA8435设计根系监测运动控制系统,给出其电路原理图。该设计可以方便地利用上位机软件控制CCD旋转和直线运动,扫描根系的全景,很好地解决了常规的监测系统监测范围有限的问题,提高了系统的实用性。实践证明,利用该方法设计的运动控制系统具有很高的推广价值和广阔的应用前景。     

轴套及轴瓦加工技术方案

本文介绍了TA2轴套及轴瓦的加工技术方案以及其搅拌轴及零部件加工通用技术要求,具有一定的借鉴意义。     

Study on Agrobacterium-Mediated Transformation of Pepper with Barnase and Cre Gene

This study was designed to control plant fertility by cell lethal gene Barnase expressing at specific developmental stage and in specific tissue of male organ under the control of Cre/lox system, for heterosis breeding of chili pepper （Capsicum annuum L.）. Chili pepper inbred lines （A, D, E, and I） were transformed with Cre gene and Barnase gene situated between loxp, separately, by means of Agrobacterium co-culture. In this study, we had established a high transformation system by extensive study of affecting factors including genotype, selection of marker, and lethal dose. Cotyledon with petiole from 9-11-day-old seeding was pre-cultured on media MR[MB（MS mineral＋vitamine B5）＋BA（6-Benzyladenine） 5.0 mg·L^-1 ＋IAA（indoleacetic acid） 1.0 mg·L^-1＋GA3（gibberellic acid） 1.0mg·L^-1＋sucrose 3%＋agar 6.5g·L^-1] for 2d. The explants were infected by Agrobacterium tumefaciens when their OD600（optical density at 600 nm）reached 0.6-0.9. After co-cultured for 4-5 d on media MC [MB＋BA5.0 mg·L^-1＋IAA 1.0 mg·L^-1 ＋GA3 1.0 mg·L^-1＋sucrose 3% ＋agar 6.5 g·L^-1＋AS （acetosyringone） 200μmol·L^-1, these cotyledons with petiole were cultured on selective differentiation medium in the media MT[MB medium supplemented with BA [5.0 mg·L^-1＋ IAA 1.0 mg·L^-1＋ GA3 1.0 mg·L^-1＋ AgNO3 5.0 mg·L^-1＋ CW （coconut water） 5% ＋ Km （kanamycin） 65 mg·L^-1＋ Cb （carbenicillin） 500 mg·L^-1＋ 3% sucrose ＋ agar 6.5 g·L^-1].The Kmr （kanamycin resistant） bud rosettes were elongated on selective elongation medium and rooted on rooting medium. PCR and Southern blotting analysis of Kmr plantlet indicated that the foreign genes had been integrated into the genome of pepper. The transgenic plants with Cre gene developed well, blossomed out, and set fruit normally. The transgenic plants with Barnase gene grew well with normal appearance of flower, but they showed different fertility from complete sterility, partial sterility to complete fertility, and similar results were obtained from in vitro pollen germination experiments.