不同性别龙胜凤鸡生长曲线的拟合与分析

为了解龙胜凤鸡的生长发育规律，本研究运用Logistic、Gompertz和Bertalanffy3种非线性模型分别对龙胜凤鸡的生长情况进行曲线拟合和分析。研究结果表明，3种模型均能较好的模拟龙胜凤鸡生长曲线，拟合度假。）均高于0．99，其中以Bertalanffy模型的拟合度最高（公、母鸡都为0．997）。因此，运用3种模型对龙胜凤鸡进行生长曲线的拟合和比较分析是可行的，本研究也为及时了解龙胜凤鸡的生长发育规律提供了参考。     

Survival and Early Development of Lodgepole Pine

The effect of site fertility, spacing and mode of regeneration on the survival and stand development of lodgepole pine, Scots pine and Norway spruce was studied in a series of experiments comprising 22 study areas in Finland. After 13-14 yrs, lodgepole pine had a mean survival of 68% in planted and 61% in seeded plots, while Norway spruce had the highest (92%) and Scots pine the second highest survival (82%). The survival of planted lodgepole pine was better the wider the spacing. Best survival was achieved on subdry and dry sites, both with planting and with direct seeding. Dominant height was not affected by spacing, but both basal area and volume at the age of 13-14 yrs were significantly higher the denser the spacing. The average difference in the value of the estimated site index H ⁵⁰ was slightly under 3 m for the superiority of lodgepole compared with Scots pine. Seeding resulted in site indices almost as high as those of lodgepole planting.     

The 1997-1998 classical swine fever epidemic in The Netherlands--a survival analysis

The aim of this analysis was to characterise the temporal pattern of infection during the 1997/98 classical swine fever (CSF) epidemic in The Netherlands and hence identify and quantify risk factors for infection in different enterprise types and areas. Survival analysis and Cox proportional hazards regression were used to describe the epidemic. Substantial differences in temporal survival patterns (herd breakdown rate) were found between areas where different control policies operated. Factors with a significant influence on the infection hazard of individual herds included: sow numbers as a percentage of total sows and fatteners (HR = 3.38 for mixed herds (0.1–60% sows) vs. fattening herds (0% sows) and HR = 2.74 for breeding herds (60–100% sows) vs. fattening herds), the number of ‘transport contacts per month’ (>0.3 vs. <0.3; HR = 4.11), pig density (pigs/km²) in the area (HR_{1000 pigs} 1.48) and herd size (HR_{100 pigs} = 1.01).

Pre-emptive slaughter in an area appeared to be associated with lower subsequent disease levels. Higher frequency of transport contacts for welfare slaughter during the epidemic, however, well regulated and controlled, was associated with a substantially higher risk of becoming infected. The positive association of a higher pig density with CSF indicates the potential importance of local spread as a factor in disease transmission and emphasizes that dilution of the pig population can contribute to reduction in CSF occurrence. This analysis suggests however, that if pre-emptive slaughter can promptly be applied effectively in an area after initial diagnosis, pig density is then not a significant factor. Mixed and breeding herds had a higher probability of becoming infected than fattening herds, possibly due to different types and frequencies of inter-herd contacts. These contacts continue to some extent during the epidemic, despite the standstill of animal movements.     

Prognostic significance of serum alkaline phosphatase activity in canine appendicular osteosarcoma

Sixty-one dogs with appendicular osteosarcoma were treated with amputation and chemotherapy of cisplatin and doxorubicin. Serum samples were obtained before and after treatment for determination of total alkaline phosphatase (TALP) activity as well as the activities of the constituent bone (BALP), liver (LALP), and corticosteroid-induced (CALP) isoenzymes. The relationship between alkaline phosphatase activities and survival was examined by Cox proportional hazards regression analysis and Kaplan-Meier log rank analysis. Mean activity of TALP, BALP, and LALP decreased significantly after treatment (P < .001). TALP and LALP activities before treatment were significantly correlated with survival (P = .006 and .001, respectively). The correlation between BALP activity before treatment and survival approached significance (P = .054). CALP activity and TALP, BALP, and LALP activities after treatment were not significantly correlated with survival. Dogs with normal pretreatment TALP and BALP activities survived significantly longer than dogs with increased pretreatment activities (P = .001 and .003, respectively). Median survival times for dogs with normal or increased TALP activities before treatment were 12.5 and 5.5 months, respectively; and median survival times for dogs with normal or increased BALP activities before treatment were 16.6 and 9.5 months, respectively. In the design of future clinical trials involving dogs with osteosarcoma, consideration should be given to stratifying the randomization according to alkaline phosphatase activity. In addition, alkaline phosphatase activity should be a factor considered by clinicians attempting to tailor the aggressiveness of adjuvant chemotherapy to the needs of individual patients or owners.     

Longevity of Swiss Brown cattle in different housing systems in Switzerland

A Weibull mixed survival model was used to study the effect of a change in the housing system on length of productive life (LPL) in dairy cows. The data set included records on 71,469 Swiss Brown cows in 1674 farms in eastern and central Switzerland. About 36% of the records were right-censored. The observation period lasted from January 1, 1988 to May 1, 2001. During this period, 517 farms changed their housing conditions from a tie-stall barn to a loose housing system. Besides a random time-dependent effect of herd–year–season, the model included fixed effects of housing system, lactation number and stage of lactation, within herd and parity production level (all time-dependent), and age at first calving (time-independent). All effects had a significant effect on LPL at a level of p < 0.001. Cows in loose housing systems showed the lowest risk ratios of being culled (0.93) compared to the reference system tie-stall barn (1.00). The highest relative culling rate (1.09) was observed within the changing period from tie-stall barn to loose housing system. Within the first three lactations, the relative culling risk increased stepwise with lactation stage. The hazard slightly increased from first until lactation number 8 and higher. The relative culling rates were highest (2.22) for cows yielding less than 90% compared to herd-mates of the same lactation number, whereas the lowest risk ratios were estimated for animals yielding more than 110% compared to their reference group (0.64). When heifers were older than 3 years at first calving, there was a tendency towards a higher relative culling risk (1.06).     

PrP genotype and lamb birth weight in a scrapie-free environment: Is there an association?

The National Scrapie Plan (NSP) for Great Britain includes selective breeding programmes. These aim to reduce the frequencies of the ovine prion protein (PrP) genotypes at high risk of developing clinical disease (principally those containing the VRQ allele) within the national flock, and select for the low-risk ARR-containing genotypes. The aim of this work was to use available data from embryo transfer programmes in guaranteed scrapie-free environments to investigate whether there was a difference in lamb birth weight associated with the lamb's PrP genotype, which might affect survival. The first analysis utilised data from 355 Cheviot lambs born between 2001 and 2004. The analysis was then repeated using a larger data set of 737 Cheviot, Dorset and Suffolk lambs born between 1999 and 2003. The most important determinants of mean lamb birth weight were litter size, sex and recipient ewe breed, plus lamb breed in the three breed analysis. In both analyses the effects of all these variables are consistent and of the same order of magnitude. In the first analysis there was evidence for an apparent increase in mean lamb birth weight for the ARR-containing genotypes of 0.3 kg (95% C.I. 0.1–0.5 kg). Whereas, in the second data set there was evidence for a decrease in mean lamb birth weight for the VRQ-containing genotypes of − 0.6 kg (95% C.I. − 0.8 to − 0.4 kg). This had been masked in the first analysis by the categorisation of these genotypes with the ARQ/ARQ genotype. Within these flocks, in a scrapie-free environment, neither finding provides support for concerns that selection for low risk ARR-containing genotypes would be detrimental to lamb birth weight, which is considered to be the most important determinant of lamb survival. Extension of this work into field flocks, where scrapie-status cannot be easily assured, will require the collection of sufficient data to adjust for known direct effects as well as any additional potential confounding effects.     

代谢调节剂对嫩枝扦插繁殖成活率的影响及其机理

  研究了红栌、樱桃砧木和石榴嫩枝扦插过程中, 喷施MHS代谢调节剂对其插条成活率、生根率和保绿时间的影响, 及其叶片中可溶性糖、可溶性蛋白质、叶绿素含量和SOD活性的变化。结果表明,MHS对扦插难、易生根植物生长均有明显促进作用。对红栌插条而言, 经生根剂浸根并喷施MHS后插条的成活率和生根率均最高, 与清水对照相比分别提高了895173%和735172% , 保绿时间延长了28185 d。插条的可溶性糖、可溶性蛋白质和叶绿素含量随处理天数的增加而呈增高趋势, 且均高于浸根处理的对照组。SOD活性在处理初期活力有所下降, 后期明显增强。由此可见, MHS是通过对插条生理生化代谢的调控来提高其成活率的。     

<Emphasis Type="Italic">Stenotrophomonas maltophilia</Emphasis>: a new potential biocontrol agent of <Emphasis Type="Italic">Ralstonia solanacearum</Emphasis>, causal agent of potato brown rot

Stenotrophomonas maltophilia was isolated from the rhizosphere of eggplant in the Nile Delta of Egypt, and its antagonistic potential against Ralstonia solanacearum race 3 biovar 2, the causal agent of potato brown rot, was in vitro evaluated on KB agar medium and in vivo on potato plants. In vitro, four isolates of S. maltophilia (PD3531, PD3532, PD3533, and PD3534) appeared antagonistic. The isolate (PD3533) was screened as the most promising antagonist for the in vivo tests. In the greenhouse, the antagonist was applied directly to soil or by bacterization of potato eyepieces. Stenotrophomonas maltophilia significantly suppressed potato brown rot in Egyptian clay soil but not in Dutch clay soil. Survival of a rifampicin and chloramphenicol-resistant S. maltophilia strain PD4560 was investigated in two pairs of clay soils, conventionally and organically managed, from Egypt and the Netherlands. The survival of S. maltophilia was significantly less in Dutch than in Egyptian soils, while the converse occurred for R. solanacearum. These results are in agreement with those obtained in the in vivo biocontrol tests. In conclusion, S. maltophilia may be useful for control of brown rot in the area where it was originally isolated, the Nile Delta in Egypt.     

Response to Insulin Treatment and Survival in 104 Cats with Diabetes Mellitus (1985–1995)

Medical records of 104 cats with diabetes mellitus were reviewed. Information from 54 cats that had multiple blood glucose concentrations evaluated at least 5 times over a minimum of 3 months, beginning at the time insulin treatment was initiated, was used to evaluate the efficacy of insulin in treating diabetes mellitus. Fourteen of 54 cats were treated with protamine zinc insulin (PZI), 26 with ultralente insulin, and 14 with lente insulin. Six, 29, and 19 cats had good, mediocre, and poor glycemic control, respectively, based on mean blood glucose concentrations, whereas 31, 21, and 2 owners thought clinical response was good, mediocre, and poor, respectively. No significant difference was found in glycemic control among cats treated with PZI, ultralente, or lente insulin. Glycemic control was significantly (P < .05) better in 33 cats without than in 21 cats with concurrent disease. All 104 cats were used to calculate survival data. Fifty-one of 104 cats were alive at the time of the study. Mean (± standard deviation [SD]) and median survival times were 24 (± 16) and 20 months, respectively, in the 51 cats still alive at the end of the evaluation, and 25 (± 4) and 17 months, respectively, in the 53 cats that had died during the period of evaluation. Pancreatic abnormalities identified in 37 cats that underwent necropsy included chronic pancreatitis (n = 17), acute to subacute pancreatitis (n = 2), exocrine pancreatic adenocarcinoma (n = 7) and adenoma (n = 1), islet cell atrophy and vacuolar degeneration (n = 27), and islet amyloidosis (n = 8). No association was found between glycemic control and islet amyloidosis or exocrine pancreatic neoplasia, or between survival time and chronic pancreatitis, islet amyloidosis, or exocrine pancreatic neoplasia. In conclusion, diabetic cats evaluated in this study showed a variable response to exogenously administered insulin, ranging from excellent to poor. By maintaining mean blood glucose concentrations under 300 mg/dL, clinical signs were improved, and owners were satisfied with insulin treatment. Concurrent potentially insulin-antagonistic diseases were common and deleteriously affected glycemic control and survival time.     

川西北高寒沙区引进桑树种试验初探

川西北地区沙化土地的急剧扩张已成为国内外关注的重大环境问题,该区域沙化治理存在植物材料单一的问题。本文从成活率、生长情况以及光合特性几个方面分析了4个桑树种在川西北高寒沙区的适应情况,结果表明4个桑树种基本能适应川西北高寒区的立地条件。