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51.
土壤铅和镉溶出伏安法检测中影响因素及其削弱方法研究进展 总被引:1,自引:1,他引:0
溶出伏安法具有分析速度快、成本低、灵敏度高等优点被广泛应用于检测土壤重金属含量,但在检测土壤重金属时,溶出伏安检测精度会受到多方面因素的影响。该文在介绍溶出伏安法工作原理的基础上,从伏安参数、试验条件和土壤物质成分三方面阐述溶出伏安法检测土壤Pb2+和Cd2+为的影响因素,解析各因素的影响机理,归纳影响削弱方法的研究进展。研究结论为:方波脉冲阳极溶出伏安法最常用于检测土壤Pb2+和Cd2+,伏安参数包括脉冲幅值、电压增量和脉冲频率,试验条件包括沉积时间、沉积电压和支持电解质种类及其pH值,土壤成分主要干扰因素包括非目标重金属和有机质。针对伏安参数和试验条件的影响可以设计优化试验有效削弱。针对非目标重金属和有机质的干扰影响,目前研究还没有提出有效的削弱方法。最后,展望了溶出伏安法检测土壤重金属的未来发展方向。 相似文献
52.
碱性蛋白酶产生菌S9发酵条件的研究 总被引:1,自引:1,他引:0
为了解大庆地区盐碱土中嗜碱微生物蛋白酶产生情况,对大庆市盐碱土中筛选出的一株碱性蛋白酶菌株S9,通过单因子试验与正交试验确定该菌株的最佳培养基为:葡萄糖2%、硫酸铵2%、酵母浸粉0.5%;最佳培养条件为:温度30 ℃,pH 9.5. 相似文献
53.
不同培养条件对微藻总脂含量和脂肪酸组成的影响 总被引:8,自引:0,他引:8
微藻细胞中脂类的数量及质量是评价其营养价值的重要指标。大量研究表明,改变培养条件及在不同的时期收获对微藻的脂肪含量和脂肪酸组成有着显著影响。本文主要从培养基成分、光强、温度、通气量和生长期5个方面综述了相关的研究成果,希望能为海洋微藻的研究和开发利用提供有价值的参考。 相似文献
54.
采用安藤芳明选择性培养基从自然发病明显的海带幼苗上分离出8株褐藻酸降解菌。研究表明,8株菌能够不同程度地降解褐藻酸钠,菌株A1和A2表现出较强的降解能力,接种1d后液体培养基即变清。温度是该菌大量繁殖的决定性因子,其生长的最适温度为20℃,最适pH7.5。褐藻酸钠质量分数为0.5%-0.6%时,不同氮源和碳源对菌体生长的影响不同,其中有机氮和铵态氮有利于菌体生长,尿素和硝酸钠抑制菌体生长;只有在培养基中含有褐藻酸钠时,菌体才能正常生长。 相似文献
55.
56.
鰤鱼诺卡氏菌培养条件及培养基的优化 总被引:1,自引:0,他引:1
对鰤鱼诺卡氏菌(Nocardia seriolea)ZJ0503的增菌培养基和生长条件进行优化。研究了温度、盐度、初始pH对鰤鱼诺卡氏菌生长的影响,并通过单因素试验对培养基的碳源、氮源和无机盐成分进行了筛选,采用正交实验法对培养基各主要成分的添加量进行了优化。结果表明,鰤鱼诺卡氏菌最适宜生长条件为温度25 ℃、盐度5、pH 6.5±0.2;经筛选,鰤鱼诺卡氏菌培养基中最佳碳源是葡萄糖,最佳氮源是酵母粉,促生长作用最强的2种无机盐是磷酸氢二钾(K2HPO4)和氯化钙(CaCl2);确立了培养基优化配方为葡萄糖20 g·L-1,酵母粉15 g·L-1,K2HPO4 0.75 g·L-1,CaCl 20.2 g·L-1(单独灭菌),氯化钠(NaCl2)5 g·L-1,pH 6.5±0.2。 相似文献
57.
Logging pattern and landscape changes over the last century at the boreal and deciduous forest transition in Eastern Canada 总被引:4,自引:0,他引:4
Forestry practices associated with the industrial era (since ~1900) have altered the natural disturbance regimes and greatly
impacted the world’s forests. We quantified twentieth century logging patterns and regional scale consequences in three sub-boreal
forest landscapes of Eastern Canada (117,000, 49,400 and 92,300 ha), comparing forestry maps depicting age and forest cover
types for early industrial (1930) and present-day (2000) conditions. Results were similar for the three landscapes, indicating
large-scale forest change during the twentieth century. In 1930, previous logging activities had been concentrated in the
lowlands and along the main hydrographical network, as compared to a more even distribution over the landscapes in 2000, reflecting
a decreasing influence of the environmental constraints on forest harvesting. In 1930, old-aged forests (>100 years) accounted
for more than 75% of the unlogged areas of the three landscapes, as compared to less than 15% for the present-day conditions.
Logging practices have thus inverted the stand age distribution of the landscapes that are currently dominated by young and
regenerating stands. The 1930 forest cover types showed a clear relationship with elevation, with conifers located in the
lowlands and mixed and deciduous stands restricted to the upper slopes. Between 1930 and 2000, 58–64% of the conifer areas
transformed to mixed and deciduous forests, such that no clear altitudinal relationships remained in 2000. We conclude that
twentieth century logging practices have strongly altered the preindustrial vegetation patterns in our study area, to the
point that ecosystem-based management strategies should be developed to restore conifer dominance, altitudinal gradients,
as well as the irregular structure inspired from old forest stands. 相似文献
58.
Softening process and total antioxidant activity were evaluated in kiwifruit differently exposed to light intensity and harvested at two different times (October 10 and November 14). Fruit was stored for 2 months at 0 °C (S1) and then maintained for a week at ambient temperature (S2). The results showed that fruit harvested later presented a faster softening rate during storage than fruit harvested earlier, even if antioxidant activity did not change. Light-exposed fruit showed higher flesh firmness than that of shaded ones. Polygalacturonase activity was higher in kiwifruit maintained for a week at ambient temperature after cool storage and, in particular the highest value was recorded in fruit harvested later. The behaviour of β-galactosidase was different: it did not show changes in fruit harvested later and significantly decreased in light- and shade-exposed fruit harvested earlier. 相似文献
59.
The effects of three set-sizes (12.5, 17.5 and 22.5 mm in diameter) and seven storage temperatures (0, 5, 10, 15, 20, 25 and 30 °C) on bolting, bulbing and seed yield in two onion (Allium cepa L.) cultivars ‘Hygro’ and ‘Delta’ were investigated. The incidence of bolting increased linearly with set-size and curvi-linearly with decreasing storage temperature. Time to inflorescence emergence and floret opening showed a curvi-linear response to storage temperature with the earliest inflorescence emergence and floret opening occurring at 5 °C and the latest at 30 °C for ‘Hygro’ and at 25 °C for ‘Delta’. Seed yield per umbel also showed a curvi-linear response to storage temperature with the lowest seed yield occurring at 30 °C for ‘Hygro’ and at 25 °C for ‘Delta’ and the highest seed yield at 5 °C. For a seed crop, storage of large sets (22.5 mm) of these cultivars at 5 °C for 120 days appeared to be optimum with 5–12% higher seed yield per umbel than that of 90 days storage. Bulb yield showed a curvi-linear response to storage temperature with the highest bulb yield occurring at 25 °C and the lowest at 5 °C. 相似文献
60.