Effect of CGRP on level of lung endogenous NO in rabbits with acute lung injury

AIM:To examine whether calcitonin gene-related peptide (CGRP) enhances nitric oxide (NO) level in pulmonary circulation blood and observe the influence of CGRP on mean pulmonary artery pressure (mPAP) in rabbits with acute lung injury (ALI) caused by oleic acid.METHODS: The level of NO was assessed by measuring the presence of nitrite in cervical artery blood by the Griess reaction, mPAP was measured with right ventricular catheter.RESULTS:The level of nitrite in cervical artery blood was significantly increased and the mPAP was markedly reduced after administration of CGRP intravenousely.CONCLUSION:CGRP enhanced the NO level of pulmonary circulation blood and reduces the mPAP significantly in rabbits with ALI.     

Biosynthesis of Depsipeptide Mycotoxins in Fusarium

The cyclic hexadepsipeptide enniatin is known as a phytopathogenic compound from Fusaria causing necrosis and wilt. The molecule consists of three alternating residues each of a branched chain amino acid and D-hydroxyisovaleric acid (D-Hiv). Enniatins are synthesized by a 347kDa multienzyme (enniatin synthetase) via a thiol template mechanism. The corresponding gene esyn1 has an open reading frame of 9393 nucleotides and harbours two modules, one responsible for D-hydroxy acid activation and one for L-amino acid activation with an integrated N-methyltransferase domain. Such methyltransferases build an homologous group among N-methyl peptide synthetases. Enniatins are synthesized by step-wise condensation of dipeptidol building blocks in an iterative manner resembling fatty acid synthesis. A key enzyme in enniatin biosynthesis is the NADPH-dependent D-2-hydroxyisovalerate dehydrogenase, that supplies enniatin synthetase with D-Hiv. Enniatins contribute to the wilt toxic character of Fusaria. Virulence was significantly reduced in F. avenaceum after disruption of the esyn1 gene.     

姬松茸菌丝活性肽的分离及其组成的初步研究

采用两次葡聚糖凝胶色谱法从姬松茸菌丝体中提取的肽类物质，初步分离获得肽，继而分析了肽的氨基酸组成和分子量。结果表明，姬松茸菌丝体中的活性肽含有丰富的氨基酸，是一种具有高F值(22．5)的寡肽，其分子量在1500～30000U之间。     

P10B抗菌肽和硫酸小檗碱对肉鸡源大肠杆菌的体外联合抗菌作用研究

为了解P10B抗菌肽与硫酸小檗碱联合抗菌作用,进行P10B抗菌肽与硫酸小檗碱联用的体外抑菌试验。试验菌株为20株肉鸡源大肠杆菌菌株。采用微量肉汤稀释法测定P10B与硫酸小檗碱对大肠杆菌的最低抑菌浓度;棋盘稀释法测定两个药的分级抑菌浓度指数,判断联合抗菌效果。结果表明,在联合药敏试验中,两药呈协同作用的占10%,呈相加作用的占85%,呈无关作用的占5%。平均分级抑菌浓度指数为0.825,两种药联和用药效应主要表现为累加作用。     

党参小包装饮片养护方法研究

[目的]通过对比不同包装、贮藏条件对党参小包装饮片内外质量的影响,建立适合党参小包装饮片的养护贮藏方法.[方法]党参饮片分别采用聚乙烯塑料、铝箔、牛皮凝膜纸袋3种包装材料包装,做真空/非真空处理后,藏于不同温湿度的环境中,定期考察外观性状、水分、浸出物、多糖及其总黄酮等指标的变化.[结果]前6个月内,各组的检测指标均无明显变化;12个月时,非真空包装样品中的多糖、总黄酮含量均呈显著变化,且室温下贮存的纸袋包装样品出现了虫蛀,色泽变暗、变深的现象;18个月时,除真空包装冷藏处理的各样品以及阴凉条件下的铝箔真空包装样品各检测指标与0个月差异不大外,其余均有明显的性状或含测指标变化.[结论]结合养护成本综合考虑,党参小包装饮片可采用真空塑料或铝箔包装,存放于阴凉干燥处.     

王家沟小流域50a土地利用动态变化分析

以经过50多a水土保持综合治理的王家沟小流域为研究对象,利用其1959—2008年土地利用数据,对该流域土地利用动态变化进行分析。结果表明,调整土地利用结构是王家沟小流域水土保持综合治理的一项关键性措施。     

生态文明视野下欠发达地区小城镇规划设计探讨——以江西省吉水县水南镇为例

小城镇是我国农村经济发展和社会进步的重要载体,也是带动一定区域内农村经济社会发展的中心,随着目前城市化速度的快速发展和小城镇规模的增大,带来各式各样的生态环境问题.文章以党的十八大提出的建设生态文明为背景,分析了国内小城镇规划建设进程中遇到的问题,并以江西省吉水县水南镇为案例,从规模预测、生态集约用地布局、田园式景观生态格局、保护与更新人文生态等内容论述了规划建设生态型小城镇的策略.     

心房钠尿肽基因敲除小鼠的鉴定

研究心房钠尿肽基因敲除小鼠(ANP-/-小鼠)的生物学及病理学特性。ANP-/-小鼠与C57BL/6小鼠饲养于SPF级环境中,观察小鼠的繁育状况及其生物学特性,选取合适时间处死小鼠,通过HE染色观察脏器形态及组织结构。ANP-/-小鼠的繁殖周期比C57BL/6长,产仔率较低;ANP-/-小鼠肝脏、肺脏、脾脏较C57BL/6小鼠的有明显炎症反应,但是C57BL/6小鼠未见明显的病理改变。提示ANP基因的缺失可能与炎症发生有关。说明ANP-/-小鼠繁殖较慢、产仔率低,且正常繁殖的普通小鼠不会引起器官的病理学改变。但作为一种在心血管、代谢疾病上的动物模型,并与炎症甚至肿瘤有可能的未知联系的转基因动物,其很有研究和探索价值。