都市农业现代化装备可持续发展研究

分析浙江省宁波市都市农业现代化装备发展的有利条件和存在问题,提出都市农业现代化装备发展的重点领域,并在此基础上从抓好农机化示范基地建设、创新农机社会化服务模式、加强农机化技术创新、建立健全农机投入机制等发面探讨宁波市都市农业现代化装备可持续发展的对策。     

贵阳市植物景观规划设计探讨

对贵阳市植物景观现状调查分析,强调在城市总体规划中绿地同其它用地一样重要,植物景观规划应是城市绿地系统规划中重要一项。植物景观设计应遵循科学性与艺术性相结合的基本原则,要注重园林植物的形式美和意境美及体现地方文化特色。指出贵阳市植物景观特色的体现重要的一点就是向自然学习,体现植物的最佳适用性,把使用乡土树种、以乔木为主的植物配置从图纸上落实在建设中,以此推行节约型、生态型、可持续发展的园林绿化。最后列出几种宜于贵阳市的植物配置模式以供参考。     

金华市农业机械化发展水平评价

为促进金华市农业机械化发展,根据现有农业机械化水平评价方法,建立了金华市农业机械化水平评价指标体系,并确定了各指标的权重.在此基础上,利用1996-2006年金华市有关农业机械化统计数据,计算出金华市历年农业机械化水平,同时分析了影响当地农业机械化发展的因素.最后,结合金华市实际,提出了促进金华市农业机械化水平提高的措施与建议.     

Effects of CENP-W down-regulation on human glioma U87 cells

AIM: To study the effect of centromere protein W (CENP-W) down-regulation on human glioma U87 cells.METHODS: Small interfering RNA (siRNA) was used to inhibit the expression of CENP-W in the U87 cells. The interference effect of siRNA was evaluated by RT-qPCR and Western blot. The proliferation of the cells was analyzed by MTT assay, BrdU staining and colony formation experiment. Transwell chamber assay was used to detect the invasion ability of the cells. The cell migration ability was measured by a scratch test. The changes of the cell cycle distribution and apoptosis were analyzed by flow cytometry.RESULTS: The results of MTT assay, colony formation experiment and BrdU staining showed that the cell proliferation and colony formation abilities in experimental group were significantly lower than those in control group and negative control group. The results of Transwell and scratch experiments showed that the migration and invasion abilities in experimental group were weaker than those in blank control group and negative control group. The results of flow cytometry analysis showed that the cell cycle distribution in experimental group was arrested in G₀/G₁ phase. The percentage of apoptotic cells in experimental group was higher than that in control group (P<0.05).CONCLUSION: Down-regulation of CENP-W expression inhibits the proliferation, migration and invasion of human glioma cells and promotes the apoptosis of the cells, suggesting that CENP-W may be a potential target of gene therapy for human glioma.     

Screening of lentiviral vectors carrying effective siRNA targeting S1P2 gene

AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×10⁵ cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency.     

基于人行为影响的住区建筑多主体集成能耗模型

在城市建筑节能研究中,政府常常依赖城市能源与碳排放模型工具制定并评估节能策略。而当前的城市建筑能耗模型受限于开发技术等因素,尚存在诸多不足,例如,考虑人行为对建筑能耗的影响,建立行为、日常活动及与环境相关的综合能耗模型。关于此类模型合理的数学形式、参数选择的方法以及不同行为的模拟方式等问题还尚待进一步研究。采用多主体（Multi-Agent）建模技术,设计集成建筑能耗模型,以住区中的居民作为模型中活动的主体,模拟使用者行为习惯、活动对建筑能耗的影响;采用ISO 13790标准模拟建筑的供热和降温需求,并在集成环境中设计统一的描述框架,以相对独立的环境模拟、行为模拟模块形式进行交互,实现热环境模拟和基于主体活动的设备能耗模拟的有机结合。研究表明,住区居民的用能行为是影响城市建筑能耗密度变化的重要因素,实现对住区主体行为特征的引导是实现城市建筑节能目标的有效途径。     

基于GIS的秦巴山区土地生态敏感性评价──以陇南山区为例

生态环境敏感性评价是进行生态功能区划的前提条件,也是判断生态系统稳定性的重要手段。进行土地生态环境敏感性评价,是落实科学发展观和生态文明理念,加快实施主体功能区规划和建设国家生态安全屏障的重要举措,对推动陇南市可持续发展和维护国家生态安全具有重要意义。笔者根据陇南市山地生态系统基本特征,运用GIS技术,从土壤侵蚀、石漠化、和地质灾害3个方面对陇南市土地生态敏感性展开单因子评价和综合评价,并对综合评价结果进行趋势面分析。评价结果表明:(1)陇南市土地生态敏感性区域差异比较明显,在东西方向上为东高西低,整体呈递减趋势,在南北方向上呈现较为明显的"n"字型,南部略高于北部;(2)运用自然断点法,将陇南市土地生态敏感性分为极敏感区、高敏感区、中敏感区、低敏感区和不敏感区,分别占市域总面积的19.18%,24.61%,25.71%,16.88%和13.61%;其中极敏感区和高敏感区主要分布在宕昌县和武都区等周边地区,该区生态敏感极强,需要加强保护,开发强度不宜过高;低敏感和不敏感区域主要分布在礼县、西和县、徽县和成县等大多数区域,可以适当进行开发。     

盐碱地公园绿化树种的选择

为更好地指导大庆市及采油七厂公园盐碱地的绿化,在规划地土壤表层化学性质测定的基础上,参照不同树木耐盐能力上限,分别对全盐量在0.1%～0.3%、0.3%～0.5%、0.5%～0.7%、＞0.7%的131个样点上绿化树种的生长状况进行了调查及分析,并进而给出了树种选择及绿化工作的建议.