南瓜新品种科栗1号的选育

科栗1号是以S31-1为母本、S4-1为父本配制而成的南瓜一代杂种。植株蔓生,长势中等;分枝较少,叶小,节间短;第1雌花节位第9节左右;嫩瓜扁圆形,瓜皮绿色覆绿色斑点和浅色纵条斑纹,老瓜皮墨绿色。嫩瓜单瓜质量0.5~1.5kg,果实横径9cm左右,纵径13cm左右,糯性好,粉质,品质佳;春季露地和秋季设施栽培嫩瓜产量1500~2000kg·(667m2)-1,早春设施栽培嫩瓜产量3000kg·(667m2)-1左右。适宜浙江省及栽培条件相似的地区推广种植。     

Influence of paricalcitol on renal tubulointerstitial fibrosis in diabetic nephropathy

AIM: To investigate the effect of paricalcitol (P) on renal tubulointerstitial fibrosis and the underlying mechanisms in diabetic nephropathy (DN).METHODS: DN rat model was induced by a single intraperitoneal injection of streptozotocin after fasting. The animals were randomly divided into 2 groups:the DN rats in paricalcitol-intervened group (group P) were injected intraperitoneally with paricalcitol dissolved in propylene glycol after the day when the model was induced successfully at a dose of 0.4 μg/kg (3 times a week); the DN rats in DN group (group D) were given isopyknic propylene glycol. Normal control group (group C) was also set up. The samples of blood, urine and renal tissue were collected after intervention of paricalcitol for 12 weeks. The biochemical indexes were measured. The renal tissues were used for pathologic observation and determining the expression of transforming growth factor-β1 (TGF-β1), Wnt-4, β-catenin and Klotho by immunohistochemistry and Western blotting. In addition, the correlation among the above indexes was analyzed.RESULTS: (1) Scr, BUN and 24 h urine protein increased significantly in group D compared with group C, while decreased in group P compared with group D (P<0.05). (2) The area of renal tubulointerstitial fibrosis increased in group D compared with group C, while decreased in group P compared with group D (P<0.05). (3) The expression of Klotho decreased, while the expression of TGF-β1, Wnt-4 and β-catenin increased in group D compared with group C (P<0.05). Compared with group D, the expression of Klotho increased, while the expression of TGF-β1, Wnt-4 and β-catenin decreased in group P (P<0.05). (4) The expression of Klotho was negatively correlated with the fibrosis area, TGF-β1, Wnt-4 and β-catenin (P<0.05).CONCLUSION: Paricalcitol inhibits renal tubulointerstitial fibrosis in DN by promoting the expression of renal Klotho, and inhibiting Wnt/β-catenin signaling pathway activation and TGF-β1 synthesis.     

兴安藜芦提取液对榆紫叶甲幼虫的防治效果

[目的]明确野生兴安藜芦对榆紫叶甲不同龄期幼虫的防治作用.[方法]以野生兴安藜芦为材料,采用70％乙醇、60％丙酮、沸水对藜芦的根、茎、叶分别进行提取,研究各提取液对榆紫叶甲2龄、3龄、4龄幼虫的防治效果.[结果]兴安藜芦不同药用部位的杀虫活性具有明显差异,以根提取物的杀虫活性最强,叶提取物的杀虫效果最差;不同提取方法的杀虫效果也有明显不同,以70％乙醇提取液的杀虫活性最强.[结论]试验结果为榆紫叶甲的生物防治提供了参考.     

秋冬萝卜新品种晋萝卜4 号的选育

晋萝卜4 号是以雄性不育系4-01A 为母本，自交系03-37-1 为父本配制而成的秋冬萝卜一代杂种，生育期80 d（天）。地上部叶丛半直立，叶片稀疏，肉质根圆柱形，表皮光滑，出土部分皮绿色，入土部分皮白色，近1/2 露出地面。含水量适中，肉质甜脆，商品性好。肉质根纵径30 cm，横径7～8 cm，单根质量1.5 kg，每667 m² 产量5 000 kg 左右。适宜山西省及其周边地区种植。     

Persimmon breeding in Japan for pollination-constant non-astringent (PCNA) type with marker-assisted selection

Oriental persimmon (Diospyros kaki) originated in Eastern Asia, and many indigenous cultivars have been developed in China, Japan, and Korea. These cultivars are classified into four groups based on their natural astringency loss on the tree and seed formation: pollination-constant non-astringent (PCNA), pollination-variant non-astringent (PVNA), pollination-constant astringent (PCA), and pollination-variant astringent (PVA). PCNA is the most desirable type because the fruit can be eaten without any postharvest treatment; therefore, one of the goals of our persimmon breeding programs is to release superior PCNA cultivars. The PCNA genotype is recessive to the other three non-PCNA genotypes, and PCNA-type F₁ offspring are obtained exclusively from crosses among PCNA genotypes. Moreover, the number of superior PCNA cross-parents have been limited. In the late 1980s, inbreeding depression became obvious, especially in terms of fruit size, tree vigor, and productivity. To mitigate the inbreeding, a backcross program using PCNA [(non-PCNA × PCNA) × PCNA] was started in 1990. This process, however, was inefficient because only 15% of the offspring were PCNA, and all offspring had to be grown to the fruiting stage. Therefore, molecular markers linked to the PCNA locus were developed for discriminating PCNA offspring. A molecular marker linked to Chinese PCNA has also been developed.     

Effects of Jiedu-Qingfei mixture on NF-κB and p38 MAPK pathways in lung tissues of rats with Mycoplasma pneumoniae infection

AIM: To observe the therapeutic effect of Jiedu-Qingfei mixture on Mycoplasma pneumoniae (MP)-infected rat lung tissues and to explore its mechanism. METHODS: SD rats (n=40) were randomly divided into 4 groups:blank control group, model group, Jiedu-Qingfei group and positive control group, with 10 rats in each group. The rats in experimental groups were slowly dripped with 1×10⁹ CFU/L MP solution into their nostrils for 4 d. One rat in each group was sacrificed for MP nucleic acid detection at the second day after inoculation, and the other rats were given gavage therapy. The rats in blank control group and model group were intragastrically given the same volume of normal saline, the rats in Jiedu-Qingfei group were given 8 mL/kg Jiedu-Qingfei mixture daily for 4 weeks, and the rats in psoitive control group were given dexmethasone sodium phosphate (0.5 mg·kg^-1·d^-1). After the experiment, the rats were killed. The serum and bronchoalveolar lavage fluid (BALF) were collected for detecting the levels of interleukin-12 (IL-12), IL-13 and TNF-α by ELISA. The right lung tissues were used for pathological observation and HE staining, while the left lung tissues were used to detect the expression of NF-κB p50, I-κBα and p38 mitogen-activated protein kinase (p38 MAPK) at mRNA and protein levels. RESULTS: The results of MP nucleic acid detection showed that all the rats except blank control group were MP nucleic acid positive, indicating that the rat model of MP infection was successfully established. On the 1st day of the treatment, the pathological scores of the lung tissues in model group and Jiedu-Qingfei group were significantly higher than those in blank control group (P<0.05). After treatment, the pathological scores of the lung tissues in mo-del group were significantly higher than those in blank control group and Jiedu-Qingfei group. The levels of IL-12 in the serum and BALF in model group were significantly lower than those in blank control group after MP infection (P<0.05), while those after treatment with Jiedu-Qingfei mixture were significantly higher than those in model group (P<0.05). The levels of IL-13 and TNF-α in the serum and BALF of MP-infected rats were increased significantly, while those after treatment with Jiedu-Qingfei mixture were significantly lower than those in model group (P<0.05). The mRNA expression levels of NF-κB p50 and p38 MAPK in model group were increased significantly (P<0.01). After treatment, the mRNA expression levels of NF-κB p50 and p38 MAPK were decreased significantly compared with model group (P<0.01). The mRNA expression level of I-κBα in model group was significantly lower than that in control group. After treatment, the mRNA expression of I-κBα in Jiedu-Qingfei group was significantly higher than that in model group (P<0.05). The protein levels of NF-κB p50 and p38 MAPK in the lung tissues of model group were significantly higher than those of blank control group. After treatment, the protein expression of NF-κB p50 and p38 MAPK was decreased significantly. The protein level of I-κBα in model group was significantly lower than that in blank control group, and after treatment with Jiedu-Qingfei mixture, the protein expression level of I-κBα was increased significantly (P<0.05). CONCLUSION: Jiedu-Qingfei mixture may attenuate lung tissue inflammation caused by MP through NF-κB and p38 MAPK pathways.     

应用~(15)N示踪探究楸树无性系对氮素的吸收和分配

为探讨楸树无性系对氮素的吸收、分配及利用特性,以2年生楸树无性系015-1、1-3、7080、1-4和004-1组培苗为试验材料,应用15N示踪技术对楸树无性系进行施肥试验。结果表明,5个楸树无性系氮肥的吸收率、利用率及分配率具有较强的一致性,氮肥利用率介于27.14%~31.24%之间。楸树无性系根和叶的肥料氮比例(Ndff)明显大于茎,楸树无性系根和叶对氮肥的竞争力较强,茎对氮肥的竞争力最弱。015-1茎部氮素分配率及无性系7080根部氮素分配率明显高于其他4个无性系;氮素分配率在各个器官中差异显著,叶片氮素的分配率最高,总体趋势为叶根茎。本研究结果为楸树氮肥的合理施用提供了理论依据。     

永安市美丽乡村建设成效、问题与对策

美丽乡村是"生产发展、生活宽裕、乡风文明、村容整洁、管理民主"的"二十字"方针贯彻落实的成果展现。在生态文明视角下建设美丽乡村是目前我国正在全力规划实施的一个重大工程。该文总结了福建省永安市美丽乡村建设所取得的成效,分析了制约其发展的因素,并提出对策建议。     

Cell direct reprogramming：a new technique for treating diabetes

Diabetes is characterized by an absolute or relative deficiency in β-cell mass, which cannot be reversed with existing therapeutic strategies. The restoration of the endogenous islet β-cells can stabilize the level of blood glucose. The islet β-cells can be obtained from the directional differentiation of stem cells, but the process is complex and has the risk of teratomas generation. Cell direct reprogramming, one terminal differentiated cell can transdifferentiate into another kind of terminal differentiated cell, which is other than directional differentiation from stem cells. Direct reprogramming gives rise to the generation of islet β-cells from one terminal differentiated cell, may be preferable for diabetes therapy because of its unique advantage.     

6种药剂对枸杞炭疽病的防治效果及其残留量的比较

为了筛选针对枸杞炭疽病的高效低残留农药,选用戊唑醇等6种药剂,就其药效及农药残留情况进行了田间比较试验。结果表明:施药后第7天,43%戊唑醇(SC)2 000倍液、40%氟硅唑(EC)6 000倍液、50%异菌脲(WP)1 000倍液、25%嘧菌酯(SC)1 000倍液、25%苯醚甲环唑(EC)1 000倍液和50%多菌灵(WP)500倍液对枸杞炭疽病的防治效果分别为97.00%、76.67%、69.33%、59.00%、56.00%和28.67%,施药后第10天的防治效果分别为92.50%、65.55%、47.41%、45.59%、45.44%和6.44%;戊唑醇的防治效果最好,氟硅唑次之。枸杞干果中戊唑醇的残留含量为2.60 mg·kg-1,低于最大残留限量;氟硅唑的残留含量为0.16 mg·kg~(-1),高于最大残留限量。6种试验药剂中只有戊唑醇符合高效低残留农药的筛选标准,可用于采果期枸杞炭疽病的防治。