锦鲤尾鳍细胞系(KF-H)的建立

本研究建立了锦鲤(Cyprinus carpio)尾鳍细胞系。染色体数目、核型及DNA含量等实验,发现锦鲤体细胞和锦鲤培养细胞无显著性差异,染色体数目和DNA含量符合比例关系,建立的锦鲤尾鳍细胞系已形成了稳定的遗传性状,命名为KF-H。     

基于多块网格技术的离心泵叶轮CFD分析

网格生成是三维紊流数值分析的重要组成部分，其网格质量对数值求解的稳定性及精度至关重要。结构化网格具有节省内存和便于节点编号等优点被广泛使用。对于几何形状复杂的离心泵叶轮通道，用单块结构化网格难以很好地进行描述。采用多块网格技术，根据各块和流场的特点，探讨了离心泵叶轮通道结构化网格划分中的一些处理方法，同时对离心泵叶轮三维紊流场进行了雷诺平均N-S方程的数值模拟，检验了所提的方法。     

AFLP analysis of genetic variability among Stylosanthes guianensis accessions resistant and susceptible to the stylo anthracnose

Stylosanthes guianensis, belonging to the genus Stylosanthes, is one of the most important tropical forage legumes and is native to South and Central America and Africa. Anthracnose, caused by the fungus Colletotrichum gloeosporioides (Penz.) Sacc., is a major constraint to the extensive use of Stylosanthes as tropical forage. Forty‐two accessions of S. guianensis were assessed with amplified fragment length polymorphism (AFLP) for genetic diversity and for resistance to anthracnose. In AFLP analysis, four selective primer combinations screened from 96 primer combinations were used to analyse these accessions, and a total of 225 clear bands were used for genetic similarity (GS) analysis, showing a 95.5% level of polymorphism on average. GS from 31.0% to 95.0% among the accessions was calculated with ntsys ‐pc software. The dendrogram was constructed with unweighted pair group method of averages (UPGMA) based on the AFLP data, and five clusters were defined at 48% GS. Two typical strains of C. gloeosporioides from Stylosanthes in China were used for anthracnose resistance screening. Most of the plant accessions showed variation in the reaction to two strains, and the correlation of resistance had a value of 0.904 (P < 0.01), suggesting common resistance to the two strains. The resistance accessions were randomly distributed in different groups of UPGMA clustering. These results demonstrate that AFLP analysis is an efficient method for evaluating the genetic diversity among S. guianensis accessions.     

Selection of soybean elite cultivars based on phenotypic and genomic characters related to lodging tolerance

Soybean lodging can result in serious yield reduction. Detecting the quantitative trait loci (QTL) associated with lodging tolerance for their further application in marker‐assisted selection (MAS) has the potential to enhance soybean breeding efficiency. In this study, a genome‐wide association analysis (GWAS) was performed to identify soybean accessions that could potentially be used to produce lodging‐tolerant varieties, based on the comprehensive evaluation of lodging scores (LS) obtained for the parental cultivar “Tokachi nagaha” and its 137 derived cultivars. Results showed that genotype, environment and genotype × environment interaction significantly influenced LS. Of the 31 significant SNPs identified, 22 were consistently detected in two or more environments and 27 SNPs were located in or close to agronomically important QTL mapped by linkage analysis. Best linear unbiased predictors (BLUPs) of LS tend to decrease with the elite alleles contained by accessions increasing. Some excellent accessions, with lower BLUPs and D_i (stability coefficients) values and more elite alleles, were selected. This study contributed to understand the genetic mechanism of lodging, providing genetic and phenotypic information for MAS.     

樟树幼林生物化学参数与高光谱遥感特征参数相关分析

高光谱遥感在植被研究中的应用使定量估算植被的生物化学参数和生物物理参数成为可能.分析了樟树幼林生物化学参数与高光谱特征变量之间的相关关系.结果表明:樟树幼林的大部分生物化学参数(叶绿素a含量、叶绿素b含量、叶绿素总含量、类胡萝卜素含量和总磷含量)与高光谱遥感特征参数(蓝边内最大的一阶微分、绿峰反射率、红边位置、蓝边面积、绿峰反射率和红谷反射率构成的比值与归一化植被指数、红边面积和蓝边面积构成的比值和归一化植被指数)之间的相关系数达到了0.01极显著性检验水平;纤维素、总氮含量与高光谱特征参数的相关系数没有达到显著性检验水平.因此,可以利用相关系数达到了0.01极显著性检验水平的高光谱特征参数建立樟树幼林生物化学参数的高光谱遥感估算模型.     

多尺度高分辨率全球土地覆被遥感产品相对一致性比较

国家及区域尺度的土地覆被信息对于解决环境演变、生物多样性保护、生态系统评价及环境建模等一系列问题起着至关重要的作用。该文从国家及区域尺度分别比较了当前全球2种最高分辨率的土地覆被遥感产品,以便解释两者在空间及专题上的一致性和异质性。结果表明,GLOBCOVER和MODIS2种产品在国家尺度上具有较好的一致性,但在区域尺度上仍有较明显的差异,特别是在中国东北和西南地区;2种产品在林地、灌木、耕地和草地间存在严重的混淆现象;总体精度和Kappa系数在全国尺度和区域尺度有着较大的差异,总体精度值从全国尺度的56.35%降为区域尺度的27.01%,Kappa系数值从全国尺度的47.09%降为区域尺度的16.57%。在全国尺度上东北区域有着最好的一致性,四川盆地的总体一致性最差;MODIS产品的类别均质性百分比明显优于GLOBCOVER,类别一致性百分比与空间一致性之间存在显著的相关性,其决定系数R2为0.724,说明产品间的类别均质性百分比差异越小,两者的空间一致性越高。该研究可为遥感产品的使用者提供准确可信的信息分布,为产品生产者有针对性改进分类算法提供科学合理依据。     

吊瓶精准输液技术对芒果产量的影响

采用吊瓶精准输液技术,在芒果树开花到成熟期按树体大小定量向树杆木质部注射营养液,以确定对芒果产量的增产效应,结果表明：芒果果数可增产23.85%,单果重可增产22.77%,总产量可增产52.06%,其产投比为3.38:1,是对照产投比（2.29:1）的1.48倍。通过定量分析和计算机模拟的方法推导出芒果精确施肥用药量的数学模型为?=5.15383(D)3.09674(g),对吊瓶输液技术导致芒果抗旱丰产的原因进行分析及讨论。     

人口城镇化与土地城镇化协调关系实证分析--以湖北省为例

选取湖北省为研究对象,在深入理解城镇化内涵的基础上,分别构建了人口城镇化与土地城镇化评价指标体系,运用SPSS17．0统计分析软件的主成分分析法确定了各指标权重值,并分别计算了湖北省1995～2012年的人口城镇化指数与土地城镇化指数,利用协调度模型逐年计算了湖北省人口城镇化与土地城镇化协调度,比照协调类型评价标准,揭示了湖北省人口城镇化与土地城镇化协调发展的时间演变特征及趋势。     

纤维素酶及其基因结构特征与功能的关系

纤维素是一种具有重要发展潜力的生物型能源.研究纤维素酶及其基因的结构和功能,阐明基因表达和降解机制是利用这一巨大资源的基础之一.本文综述了纤维素酶及其基因结构和功能研究的最新进展,并对其发展前景进行了分析.     

转基因玉米双抗12-5对根际土壤酶活性及 微生物多样性的影响

采用田间试验研究了转基因玉米瑞丰1号-双抗-12-5(RF1-12-5)种植对根际土壤酶活性、微生物群落的影响,可为RF1-12-5的环境释放和商业化应用提供科学的安全性评价数据支持。研究结果表明:①在5个生育期内,RF1-12-5与其非转基因对照品种瑞丰1号(RF1)根际土壤碱性蛋白酶、脲酶和酸性转化酶活性均没有显著性差异;RF1-12-5根际土壤过氧化氢酶活性在收获期、碱性磷酸酶活性在乳熟期显著低于RF1,其他生育期差异不显著。②在5个生育期内,RF1-12-5与RF1根际土壤微生物群落的Shannon多样性指数、McIntosh均匀度指数和Simpson优势度指数均不存在显著性差异,主成分分析未发现RF1-12-5与RF1根际微生物功能多样性存在规律性差异。