青山羊种公羊能量需要量的研究

12只18月龄青山羊种公羊随机分为2组,通过饲养试验、气体代谢试验及屠宰试验,研究种公羊能量代谢规律及需要量。试验表明(1)青山革种公羊的MEm为507W~(0.75)KJ,代谢能的增重效率K_G为0.157;(2)绝食产热为282.4KJ/kgW~(0.75),非配种期一天产热量(HP)与食入代谢能(MEI)呈强直线相关(P<0.01):HP/W~(0.75)=186.2+0.45MEI/W~(0.75);(3)本试验中,高能量水平对精清生化指标、性行为及精液品质各项指标影响差异不显著。综合试验结果,按析因法得出青山羊种公羊非配种期与配种期代谢能需要量分别为:ME_R=507W~(0.75)+36⊿W,ME_R=(507+122n)W~(0.75)。     

双峰驼精液的某些特点及化学成分分析

本研究从10峰公驼采精158次,研究了双峰驼精液的基本特征及主要化学成分。结果表明,双峰驼的射精量为4.35±1.86毫升,采精后精子活力为0.78±0.19,精子浓度为5.59±1.20亿/毫升,精液的pH为7.37±0.06。此外还对精清中的葡萄糖、乳酸、磷、钙、钾、钠、氯、镁及总氮含量进行了测定。     

水貂人工授精的研究

1983～1986年对水貂进行电刺激,阴道采精94头次,成功81次,成功率86.2％。测得公貂射精量为0.11±0.01毫升,精子密度10.70±2.99千万,精子活力0.716±0.021。研究制定水貂精液冷冻程序。冷冻24次,解冻后精子复苏率为83.7％。1985年用冻精按常规从阴道给母貂输精,产出2窝健康仔貂(共输5只),每窝产仔2.0±1.0只。     

二氢吡啶与VE对布尔山羊精液品质的影响

本试验以二氢吡啶（每日每头50mg）和VE（每日每头15mg）以及二者联合使用喂布尔山羊种公羊,现察布尔山羊精液品质的变化情况,结果表明,单纯二氢吡啶或二氢吡啶与VE显著提高公羊射精量和精子活力（P＜0.01＝,显著降低精子畸形率（P＜0.01＝;单纯饲用VE 对公羊精液品质明显（P＞0.05）     

Isolation of six isoflavones from Semen sojae praeparatum by preparative HPLC

A method for the isolation of six isoflavones (genistein, genistin, daidzein, daidzin, glycitein and glycitin) with high purity from Semen sojae praeparatum, a famous traditional Chinese medicine, by preparative HPLC is described.     

单复冠基因对白羽丝毛乌骨鸡体尺性状与精液品质的影响

为观察单、复冠基因对白羽丝毛乌骨鸡的体尺性状以及精液品质的影响,寻找体尺性状和精液品质间的相互关系,对44只55周龄单、复冠白羽丝毛乌骨鸡种公鸡的体尺与精液品质进行了测定,结果表明：单冠乌鸡的体尺均值均大于复冠乌鸡;单、复冠乌鸡间精液品质无明显差异;单、复冠乌鸡的体尺性状与精液品质间的相关性均无达到显著水平;单冠乌鸡的精液品质与体尺性状间的相关性多为负相关。故在乌鸡的选育中,不仅要重视单冠基因在体尺性状方面的明显优势,还应该特别注意单、复冠乌鸡在精液品质与体尺性状相关性方面的差别。     

Motility and Fertility Evaluation of Thawed Frozen Stallion Semen After 24 Hours of Cooled Storage

Breeding mares with cryopreserved semen requires specialized equipment for storage and thawing and more intensive mare management. The objectives of this study were (1) evaluate the longevity of frozen stallion semen once it had been thawed, extended, and maintained at 5°C for 48 hours in a passive cooling container, and (2) determine fertility potential of frozen semen that had been thawed, extended, and used to inseminate mares after 24 hours of cooled storage. Eight ejaculates were collected and aliquots were cooled in either INRA96 and CryoMax LE minus cryoprotectant at a concentration of 50 million total sperm/mL. The remainder of the ejaculate was frozen in CryoMax LE extender at a concentration of 200 million total sperm/mL. Semen was thawed using 1 of 3 thawing protocols, and diluted to a concentration of 50 million total sperm/mL in either INRA96 or CryoMax LE minus cryoprotectant and cooled to 5°C. Sperm motility was evaluated at 24 and 48 hours. Eight mares were inseminated over two estrous cycles using frozen semen that had been thawed, extended in INRA96, and cooled for 24 hours. There was no difference in progressive motility at 24 or 48 hours of cooled-storage post-thaw between the 3 thawing protocols. An overall per cycle pregnancy rate of 56% (9/16 cycles) was achieved using frozen-thawed semen that had been extended and cooled for 24 hours. In summary, frozen stallion sperm was thawed, extended, and cooled to 5°C for 24 hours and still maintained adequate (>30%) sperm motility and fertility.     

Changes in Blood and Semen Quality of Bulls after Artificial Infection with Mycoplasma wenyonii

[目的]验证温氏枝原体对种公牛血液和精液品质的影响。[方法]将10头健康的鲁西黄牛的种公牛随机分成2组,试验组分别于试验当天和第28天静脉注射温氏枝原体,从临床症状、血液涂片镜检、血液生理生化、精液品质和阴囊变化等方面,综合评价温氏枝原体对种公牛的影响。[结果]除个别指标外,试验组临床检查基本正常,未出现明显的血液和精液品质异常。[结论]该研究表明人工感染温氏枝原体对种公牛毒力较低,致病性不强,对血液和精液品质无明显异常。     

山羊精液冷冻保存技术研究

[目的]探索山羊精液的冷冻方法。[方法]以甘油为冷冻保护剂,采用细管法冷冻保存山羊精液,探讨甘油浓度和添加时间、冻结方法和解冻温度对山羊精液冷冻效果的影响。[结果]在稀释液中添加6%和7%甘油的处理组中,冻后精子活率与复苏率均显著高于添加3%、5%甘油的处理组。配液时添加6%甘油的处理组中冻后精子活率与复苏率均显著低于在4℃平衡后添加的处理组。把平衡的山羊精液分别在离液氮面3 cm处熏蒸9 min和-80℃冰箱中冻结9 min,前者的精子复苏率显著低于后者。在解冻温度分别为40、45和50℃的处理组中精子冻后活率与复苏率显著高于解冻温度为55和60℃的处理组,说明解冻温度以40～50℃为宜。[结论]在山羊精液冷冻保存中,甘油的最佳添加时间为4℃平衡后冻结前。     

双峰驼的人工授精及精液冷冻技术

 骆驼配种采用人工授精。其操作技术基本同于马、牛，采输精借用牛的假阴道和输精器。原精用12%蔗糖溶液或7%葡萄糖溶液稀释，实行阴道输精，每次输0.5毫升以上，有效精子数不低于0.8-1.0亿，受胎率达80%（42/52），产羔率75%（39/52）。驼精以蔗甘液稀释和铜纱网漂浮液氮熏蒸慢速降温法。制安瓿冻精65批665支，平均活力0.4，高者达0.5；制颗粒冻精1200余粒，活力0.3-0.5。解冻后室温（14—16℃）保存，平均存活86小时，长者达190小时。1983-1986年共输精92峰，受胎率为76.1%（70/92），产羔率为61.96%（57/92）。在第一次输精的同时，给发情母驼肌注LRH-A诱导排卵，排卵率可达94.4%。