蝴蝶兰SRAP反应体系的建立与优化

以蝴蝶兰嫩叶提取的DNA为材料,蝴蝶兰SRAP反应体系中的重要参数Mg2＋、Taq酶、模板DNA及随机引物,建立了一套适合蝴蝶兰基因扩增的SRAP反应体系：25μL的反应体系中Mg2＋浓度为2.0mmol/L,Taq酶1.0U,DNA模板40ng,上下游引物0.8mmol/L。该体系扩增条带清晰,重复性好,有望在蝴蝶兰属植物的遗传育种研究中运用。     

QTL Mapping of Isoflavone,Oil and Protein Contents in Soybean （Glycine max L. Merr.）

Soybean （Glycine max L. Merr.） is the world＇s foremost source of edible plant oil and proteins, meantime, the biologically active secondary metabolites such as saponins and isoflavones are benefit to human health. The objective of this study was to identify quantitative trait loci （QTL） and epistatic interactions associated with isoflavone, protein, and oil contents in soybean seeds. An F13 recombinant inbred line （RIL） comprising 474 lines was derived from a cross between Jindou 23 and Huibuzhi cultivars. SSR technique was employed for mapping of the QTLs. The QTLs for isoflavone, protein, and oil contents were analyzed and 23 QTLs were detected based on the constructed linkage map. Six QTLs for isoflavone content were localized in linkage groups J, N, D2, and G, eleven QTLs for oil content were localized in the linkage groups A1, A2, B2, C2, and D2, and six QTLs for protein content were localized in linkage groups B2, C2, G, and H1. The correlative analysis demonstrated that the isoflavone content had significant correlation with protein content, while significantly negative correlations was existed between oil and protein content, and significantly positive correlations was existed between protein and oil content. All these findings have laid an important basis for the marker assisted breeding in soybean. The phenotypic correlations of quantitative traits may be resulted from the correlation of the QTL controlling those traits.     

Analysis of the Genetic Relationships in Chinese Ziziphus with SRAP Markers

The phylogenic and genetic relationships in germplasm resources of Chinese Ziziphus were studied at molecular levels, for providing new molecular evidences of classification, protection, and utilization of germplasm resources of Ziziphus. The sequence-related amplified polymorphism （SRAP） was assessed to analyse the genetic relationships among 14 species of Ziziphus, 11 cultivars of Z. jujuba Mill., and one outgroup. A total of 580 DNA bands were amplified by 19 selective primers, 570 of which （98.28%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 30. The genetic similarities of 26 sample materials were between 0.22 and 0.99. UPGMA method cluster analysis showed that 26 sample materials were classified into six cluster groups with the genetic similarity of 0.28. The results showed that SRAP technique is efficient in studying genetic relationships among Ziziphus, Z. jujuba Mill. and Z. acidojujuba C. Y. Cheng et M. J. Liu which should be treated as one species, and further infraspecific classification of Z. jujuba Mill. should be classified into two subspecies; the scientific names of new taxa, Chinese jujube, and wild jujube were Ziziphus jujuba Mill. subsp, jujuba and Ziziphus jujuba Mill. subsp, spinosa （Bunge） J. Y. Peng, X. Y. Li et L. Li, respectively. Z. xiangchengensis Y. L.Chen et P. K. Chou, Z. montana W.W. Smith and Z. mairei Dode might be originated from same ancestry. Z xiangchengensis Y. L. Chen et P. K. Chou and Z. montana W.W. Smith should be treated as one species. The infrasubspecific taxon of Z. jujuba Mill. was not suitable to set up varieties.     

苎麻多胚苗遗传多样性的SRAP标记分析

为分析苎麻多胚苗的遗传多样性,以靖西青麻和中苎1号杂交收获F1代种子,从中筛选出 21对双胚苗、3株三胚苗和1株单胚苗,用SRAP标记对这些材料及其亲本进行遗传分析.结果表明:筛选出的24个引物组合共扩增出159个稳定的多态性条带,平均每个组合6.8个;48份材料中成对遗传相似系数为0.360～0.876,在遗传相似系...     

利用SRAP分子标记技术鉴定大白菜种子纯度

以大白菜品种多抗3及其父母本为研究材料,利用SRAP（相关序列扩增多态性）分子标记方法,通过对54对SRAP标准引物的筛选,获得了能区分大白菜多抗3及其亲本的引物Me4F/Em3R。采Me4F/Em3R引物对对5份多抗3系列商品种子纯度进行检验,种子的纯度分别为75.5%、72%、80.5%、68%和73%,与田间种植...     

三叶草遗传多样性的SRAP分析

利用SRAP(sequence related amplified polymorphism)标记技术对红三叶(Trifolium pretense)、白三叶 (T.repens)、杂三叶(T.hybridum)及白三叶中的叶型变异植株共11份材料的遗传多样性以及亲缘关系进行研究。结果表明,40对引物共产生了426个多态性条带,平均每对引物产生10.7个多态性条带,多态性位点百分率为53.8%。7份白三叶的遗传相似系数变化范围为0.455～0.927,平均相似系数为0.854,白三叶叶型变异植株有特异性条带出现;3份红三叶的遗传相似系数变化范围为0.757～0.837,平均相似系数为0.791,说明白三叶和红三叶种内均存在一定的遗传多样性。种间聚类表明,杂三叶与红三叶的亲缘关系较为接近。表明SRAP技术可有效地用于三叶草的种间种内亲缘关系、种质资源鉴定和遗传多样性分析。     

利用ITS和SRAP分子标记对中国白灵菇和欧洲侧耳属菌株进行亲缘关系鉴定

为了评价白灵菇的分类地位,采用内转录间隔区(ITS)序列分析和相关序列扩增多态性(SRAP)两种分子标记技术,系统分析了包括中国白灵菇商业菌株和欧洲Pleurotus nebrodensis菌株在内的34株侧耳属菌株的DNA多态性.ITS序列分析结果显示:供试菌株的ITS序列变异不仅表现在碱基的变异,也表现在区域长度的变异.利用7对扩增条带清晰、多态性较丰富、稳定性较好的引物对34个供试菌株进行SRAP扩增,共得到420条条带,且均为多态性条带.ITS序列分析和SRAP分析结果均显示:白灵菇和Pleurotus nebrodensis具有很近的遗传关系.将ITS和SRAP两种标记联合使用能得到更好的结果.     

正交设计优化花菜类SRAP分子标记体系的研究

采用正交设计,从Mg^2＋、dNTPs、引物三种因素3个水平对青花菜SRAP反应体系进行优化,确立适合青花菜的SRAP反应体系,并在6个青花菜和花椰菜品种中进行验证。结果表明：20μ/L反应体系中适宜体系的浓度dNTPs为0．2mmol／L、Mg^2＋为1．25mmol／L、上下引物各0．4μmol／L、DNA为20ng、Taq酶1．2U、退火温度为50℃。     

长毛兔遗传基因库的新成员——荥经长毛兔

荥经长毛兔是由四川省荥经县畜牧局、四川农业大学、雅安市畜牧局联合培育的中型细毛型长毛兔新品系,将分子遗传标记技术与BLUP法结合,选用雅荥长毛兔类群(含中系、德系安哥拉兔血缘)为母本、镇海长毛兔为父本的杂交     

红松SRAP-PCR反应体系的优化

通过单因素试验,对红松SRAP—PCR反应体系的主要影响因子（Taq DNA聚合酶、Mg^2＋、模板DNA、dNTPs、引物浓度）进行了优化筛选,得出红松SRAP—PCR反应的最佳体系：20μL反应体系中,模板90ng,Mg^2＋1．0mmol／L,dNTPs0．15mmol／L,Taq DNA聚合酶1．5 U,引物0．3～0．4μmol／L。