基于稀疏图像的真实树交互式建模方法

真实环境中树的三维重建可在虚拟现实、景观设计及农林业应用方面发挥重要作用,为解决真实环境中树的三维重建问题,该文提出一种基于稀疏图像的交互式建模方法。在自然环境下采集2幅相差90°的树图像及对应4～7幅中间图像,采用交互式编辑方法在夹角相差90°的1幅图像上获取各级树枝二维投影位置及粗度信息,再通过中间图像找到各级树枝在另一幅图像上的匹配树枝,并交互式调整树枝位置信息,然后进行透视校正,生成树枝三维几何模型,最后根据叶序规则添加树叶完成重建。通过对苹果树、樱桃树和枫树的重建结果表明,该方法交互性好,对图像拍摄数量与角度要求不高,重建时间在55～125 min之间,且能较好保持树的拓扑结构,可为虚拟植物建模、虚拟修剪试验和植物拓扑结构分析等提供参考。     

SlSP5G3基因对‘Micro-Tom’番茄开花和产量的影响

番茄(Solanum lycopersicum L.)开花时间直接决定后期的产量和经济效益。为了探讨番茄 SlSP5G3基因功能,以‘Micro-Tom’番茄为试验材料,观察 SlSP5G3基因时间和空间的变化规律及对番茄开花和产量的影响。结果发现 SlSP5G3的表达响应光周期变化,主要在叶片中表达,且表达量不受生长发育时期的影响。在‘Micro-Tom’番茄中过表达 SlSP5G3,获得过表达植株L8和L12。与野生型植株相比,L8和L12植株开花所需叶片数分别增加2.34和2.67片;叶片鲜质量分别增加98.43%和76.89%;叶片干质量分别增加62.79%和41.86%;果实单果质量分别增加49.65%和36.13%;单株产量分别增加34.50%和26.74%,并且 SlSP5G3过表达植株还显著提升了上游开花转录因子 SlCDF3的表达量。 SlSP5G3通过调控开花时间,改变植株生长形态,对提升植株产量产生重要影响。     

基于 Polysun 5太阳能干燥系统的设计与仿真

利用太阳能对农副产品进行干燥,是太阳能热利用的方式之一。其与自然晾晒相比,能有效地提高干燥效率和干燥品质;与常规热风干燥相比,可以有效节省煤电的消耗,减少温室气体的排放量。为此,首先利用传热学相关理论对枸杞干燥过程进行理论计算;然后,根据得到的基础数据,利用Polysun 5辅助设计一种满足枸杞干燥需要的太阳能-燃气锅炉组合干燥系统;最后,对该系统进行仿真分析。仿真结果证明,与常规热风干燥系统相比,该太阳能组合干燥系统在1个干燥周期内,可减少二氧化碳排放28．22kg,节省能源费用180元,节电390kW· h,经济性较好。     

茶树叶片内核酮糖二磷酸羧化酶酶蛋白水平的研究

茶树叶片内核酮糖二磷酸羧化酶蛋白水平,随着新梢叶片的逐渐成熟而增加,当叶龄在33～48天,叶片完全成熟时,蛋白水平达最高值并持续稳定,以后随叶龄的进一步增大而呈下降趋势;叶位试验也表现出相同的变化规律,以芽下第4～6叶位叶中的蛋白水平为稳定最高;在年生育过程中,不同品种春梢叶在春、夏、秋三季中的蛋白水平均以夏季最低,但福鼎大白茶表现为春>秋,而龙井43与龙井长叶表现为秋>春;不同品种春、夏、秋三季新梢叶中的核酮糖二磷酸羧化酶蛋白水平差异,福鼎大白茶与政和大白茶表现为春>秋>夏的规律,龙井43、龙井长叶、迎霜、水古四品种则以秋季最高,春、夏差异不大.     

共表达O型FMDV衣壳蛋白和绿色荧光蛋白重组腺病毒的构建

为构建表达O型口蹄疫病毒(FMDV)的P12A3C基因及GFP基因的重组腺病毒rAdV-P12aEGFP2a3C,本研究以FMDV的2A基因序列为Linker,将报告基因EGFP插入FMDV的P12A与3C之间。重组腺病毒感染HEK-293细胞后可以观察到绿色荧光,表明EGFP蛋白获得表达。应用FMDV的VP2单克隆抗体4B2对重组病毒感染细胞进行western blot检测,反应条带与FMDV衣壳蛋白VP0和VP3的分子量大小相符,表明FMDV的完整衣壳蛋白和3C蛋白酶也均获得表达,而且EGFP的插入并未影响P1蛋白的表达和3C蛋白酶对P1的正确切割。重组腺病毒的生长特性分析表明,EGFP的插入也未影响该重组腺病毒的增殖特性。上述研究结果显示,表达FMDV衣壳蛋白P12A3C的重组腺病毒可以作为载体,以2A蛋白作为Linker表达一个小分子蛋白,为改进以腺病毒为载体的口蹄疫基因工程疫苗提供了新思路。     

猪繁殖与呼吸综合征病毒GP5、M蛋白基因的克隆及其基因疫苗构建

参照GenBank中猪繁殖与呼吸综合征病毒(PRRSV)美洲型代表株VR2332 GP5和M蛋白基因序列,设计并合成2对引物,用RT-PCR方法分别扩增出PRRSV野毒株GP5和M蛋白基因603,525bp片段,并将其分别克隆到pMD18-T载体。测序正确后,将GP5和M蛋白基因分别克隆到真核表达载体pEGFP-C1上,成功构建基因疫苗表达载体pEGP5-C1和pEM-C1。小鼠免疫试验证实,这些基因疫苗质粒可以诱导小鼠产生特异性抗体,并在二免后1周开始检测到特异性淋巴细胞增殖反应。     

H5N2与H5N1禽流感油乳灭活疫苗免疫鹅群对H5N1流行株攻毒的保护效力及母源抗体对免疫效力的干扰

应用H5N2和H5N12种类型禽流感油乳灭活疫苗分别免疫鹅群,观察比较了二者对H5N1型高致病性禽流感病毒的攻毒保护效力。结果表明,在同等剂量免疫条件下,从发病率、死亡率和泄殖腔排毒规律3项指标综合评价来看,H5N1灭活苗水禽免疫组对高致病性禽流感H5N1流行株攻毒的保护效率较H5N2灭活苗理想,且水禽禽流感母源抗体对灭活苗免疫具有一定的干扰作用。     

Avian Influenza A(H5N1) Virus Outbreak Investigation: Application of the FAO‐OIE‐WHO Four‐way Linking Framework in Indonesia

WHO, FAO and OIE developed a ‘four‐way linking’ framework to enhance the cross‐sectoral sharing of epidemiological and virological information in responding to zoonotic disease outbreaks. In Indonesia, outbreak response challenges include completeness of data shared between human and animal health authorities. The four‐way linking framework (human health laboratory/epidemiology and animal health laboratory/epidemiology) was applied in the investigation of the 193rd human case of avian influenza A(H5N1) virus infection. As recommended by the framework, outbreak investigation and risk assessment findings were shared. On 18 June 2013, a hospital in West Java Province reported a suspect H5N1 case in a 2‐year‐old male. The case was laboratory‐confirmed that evening, and the information was immediately shared with the Ministry of Agriculture. The human health epidemiology/laboratory team investigated the outbreak and conducted an initial risk assessment on 19 June. The likelihood of secondary cases was deemed low as none of the case contacts were sick. By 3 July, no secondary cases associated with the outbreak were identified. The animal health epidemiology/laboratory investigation was conducted on 19–25 June and found that a live bird market visited by the case was positive for H5N1 virus. Once both human and market virus isolates were sequenced, a second risk assessment was conducted jointly by the human health and animal health epidemiology/laboratory teams. This assessment concluded that the likelihood of additional human cases associated with this outbreak was low but that future sporadic human infections could not be ruled out because of challenges in controlling H5N1 virus contamination in markets. Findings from the outbreak investigation and risk assessments were shared with stakeholders at both Ministries. The four‐way linking framework clarified the type of data to be shared. Both human health and animal health teams made ample data available, and there was cooperation to achieve risk assessment objectives.     

Effects of modulatory agents on neurally-mediated responses of trout intestinal smooth musclein vitro

Mediators and mechanisms responsible for the inhibitory modulation of trout intestinal smooth muscle were examined using a series of putative mediators and substances known to modulate neurotransmission in mammalian systems. Frequency response relationships to transmural stimulation and concentration response relationships to 5-hydroxytryptamine, carbachol, and substance P were established on paired segments of rainbow trout intestinein vitro in the presence and absence of putative modulatory agents. Modulation of neurally-mediated contractions of trout intestine was achieved with dibutyryl cyclic AMP and forskolin, agents that increase intracellular levels of cyclic AMP. The effect appears to be at the level of the smooth muscle, since the adenylate cyclase activator, forskolin, inhibited muscarinic and serotoninergic contractions as well as transmurally stimulated contractions. Substance P-induced contractions were unaffected by forskolin. The endogenous agonists/neurotransmitters which would increase cyclic AMP levels in rainbow trout intestinal smooth muscle are as yet unknown. The effects do not appear to be modulated by vasoactive intestinal peptide (VIP), calcitonin, calcitonin gene-related peptide (CGRP), or agents that activate -adrenoceptors. Prostaglandin E₂ (PGE₂) and ₂-adrenergenic agonists are possible agents which will decrease contractility of the smooth muscle. They were only active in the proximal intestine and on transmurally stimulated contractions. The effects of both PGE₂ and ₂-agonists appear to be prejunctional, decreasing release of contractile neurotransmitters in the enteric nervous system.