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101.
设计、试制了一种可以用于无土栽培循环营养液消毒的紫外线消毒系统,并对其杀灭黄瓜枯萎病病原菌的性能和效果进行了实验室研究。先将黄瓜枯萎病病原(Fusarium oxysporum f.sp.cucumerinum)菌株接种于PDA培养基上,培养后将其加入到营养液中进行紫外线照射。通过对紫外线照射前后的病原浓度进行计数,发现紫外线对黄瓜枯萎病病原有较好的杀灭效果。在营养液紫外线透射率(T10)为96.65%、病原浓度为6.87×102conidia/ml、最大流量为36 L/min时,灭菌率为100%;在营养液紫外线透射率(T10)为95.42%、病原浓度为1.14×105conidia/ml、最大流量为36L/min时,灭菌率为100%;在T10降低为11.58%、病原浓度为1.44×104conidia/ml、最大流速为35L/min时,灭菌率也在50%以上。 相似文献
102.
103.
10kV架空线路常见故障及防范措施 总被引:2,自引:0,他引:2
从10kV架空线路的常见故障类型进行分析,并对10kV架空线路的反事故防范措施进行探讨,提高10kV架空线路的安全运行水平,提高供电可靠性。 相似文献
104.
通过10%单嘧磺隆可湿性粉剂田间喷雾试验表明,其对小麦、玉米的出苗和生长发育均无不良影响,但严重阻碍复种和后茬油菜等双子叶作物的出苗和生长发育,生产上应避免在10%单嘧磺隆可湿性粉剂施药田复种或后茬种植双子叶作物.同时表明对春小麦田阔叶杂草有较好的防除效果,优于目前生产上常用药剂10%苯磺隆可湿性粉剂,且对小麦、玉米安全,具有推广应用前景.建议用药时间为小麦3叶1心、杂草2叶1心期,用药量为225 g/hm2对水900 L. 相似文献
105.
本研究旨在探究光信号如何调控草莓花青素苷合成的分子机制,利用同源克隆法从3个不同颜色的草莓品种(‘红颜’,‘桃熏’,‘白雪公主’)中分离得到FaMYB10基因及其启动子序列,采用酵母双杂交系统验证FaMYB10的转录激活能力及其与FaCOP1的蛋白互作关系,利用红颜草莓FaMYB10基因的启动子序列构建酵母单杂交诱饵载体pHIS2-ProFaMYB10,并验证其在酵母Y187中的自激活现象。结果表明,经测序显示3个不同颜色的草莓品种FaMYB10基因的CDS区域序列完全一致为702 bp,但是在启动子区域存在不同差异。酵母双杂交实验表明Fa MYB10在Y2HGold中存在转录激活能力,FaMYB10同FaCOP1存在蛋白质与蛋白质互作关系。在Y187宿主内,发现当3-AT浓度达到60 mmol/L无法抑制FaMYB10基因启动子带来的自激活现象。本研究结果为理解草莓通过光信号传导调控下游FaMYB10,进而影响花青素苷合成的分子机制提供一定理论参考。 相似文献
106.
双亚10号亚麻新品种的选育 总被引:2,自引:0,他引:2
双亚10号是以(78—97×奥尔沙)×(85—1832×FR2)复合杂交育成的,具有高纤、优质、抗旱、抗倒伏和抗病优点。区域试验每公顷原茎、长纤维和种子产量分别为6068.7kg、975.1kg和741.9kg,分别比对照品种黑亚7号增产6.6%、24.5%和18.4%。生产试验每公顷原茎、长纤维和种子产量分别为5326.1kg、861.0kg和449.1kg,分别比对照品种黑亚11号增产8.5%、23.7%和10.9%。长麻率20.6%、全麻率29.9%,纤维强度26.7kg。 相似文献
107.
Kirmizibekmez H Ariburnu E Masullo M Festa M Capasso A Yesilada E Piacente S 《Fitoterapia》2012,83(1):130-136
From the MeOH extract of Sideritis trojana, a new iridoid glycoside, 10-O-(E)-feruloylmelittoside (1) was obtained in addition to four known iridoid glycosides [melittoside (2), 10-O-(E)-p-coumaroylmelittoside (3), stachysosides E (4) and G (5)]. Moreover, five phenylethanoid glycosides [verbascoside (6), isoacteoside (7), lamalboside (8), leonoside A (9), isolavandulifolioside (10), three flavone glycosides (isoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (11), 4'-O-methyisoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (12), 3'-hydroxy-4'-O-methyisoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (13) and a benzylalcohol derivative (di-O-methylcrenatin) were obtained and identified. The structures were elucidated on the basis of NMR and HRMS data. All compounds were tested for their antioxidant activity by in vitro TEAC assay and some of them exhibited moderate activity (0.97-1.44 mM) when compared with the reference compound (quercetin 1.86 mM). Glycosides 6-13, the most active compounds in the TEAC assay, were also tested by flow cytometry to evaluate their ability to affect the levels of reactive oxygen species (ROS) in human prostate cancer cells (PC3). 相似文献
108.
Dolastatin 10 (Dol-10), a leading marine pentapeptide isolated from the Indian Ocean mollusk Dolabella auricularia, contains three unique amino acid residues. Dol-10 can effectively induce apoptosis of lung cancer cells and other tumor cells at nanomolar concentration, and it has been developed into commercial drugs for treating some specific lymphomas, so it has received wide attention in recent years. In vitro experiments showed that Dol-10 and its derivatives were highly lethal to common tumor cells, such as L1210 leukemia cells (IC50 = 0.03 nM), small cell lung cancer NCI-H69 cells (IC50 = 0.059 nM), and human prostate cancer DU-145 cells (IC50 = 0.5 nM), etc. With the rise of antibody-drug conjugates (ADCs), milestone progress was made in clinical research based on Dol-10. A variety of ADCs constructed by combining MMAE or MMAF (Dol-10 derivatives) with a specific antibody not only ensured the antitumor activity of the drugs themself but also improved their tumor targeting and reduced the systemic toxicity. They are currently undergoing clinical trials or have been approved for marketing, such as Adcetris®, which had been approved for the treatment of anaplastic large T-cell systemic malignant lymphoma and Hodgkin lymphoma. Dol-10, as one of the most medically valuable natural compounds discovered up to now, has brought unprecedented hope for tumor treatment. It is particularly noteworthy that, by modifying the chemical structure of Dol-10 and combining with the application of ADCs technology, Dol-10 as a new drug candidate still has great potential for development. In this review, the biological activity and chemical work of Dol-10 in the advance of antitumor drugs in the last 35 years will be summarized, which will provide the support for pharmaceutical researchers interested in leading exploration of antitumor marine peptides. 相似文献
109.
110.
Shi-chao WANG Jin-zhou WANG Ya-wen ZHAO Yi REN Ming-gang XU Shu-xiang ZHANG Chang-ai LU 《农业科学学报》2019,18(11):2619-2627
The contribution percentage of inherent soil productivity (CPISP) refers to the ratio of crop yields under no-fertilization versus under conventional fertilization with the same field management. CPISP is a comprehensive measure of soil fertility. This study used 1 086 on-farm trials (from 1984–2013) and 27 long-term field experiments (from 1979–2013) to quantify changes in CPISP. Here, we present CPISP3 values, which reflect the CPISP states during the first three years after site establishment, for a series of sites at different locations in China collected in 1984–1990 (the 1980s), 1996–2000 (the 1990s), and 2004–2013 (the 2000s). The results showed that the average CPISP3 value for three crops (wheat, rice, and maize) was 53.8%. Historically, the CPISP3 in the 1990s (57.5%) was much higher than those in the 1980s (50.3%), and the 2000s (52.0%) (P≤0.05). Long-term no-fertilization caused CPISP levels to gradually decline and then stabilize; for example, in a mono-cropping system with irrigation, the CPISP values in Northwest and Northeast China declined by 4.5 and 4.0%, respectively, each year for the first ten years, but subsequently, the CPISP values stabilized. In contrast, the CPISP for upland crops in double-cropping systems continued to decrease at a rate of 1.1% per year. The CPISP for upland-paddy cropping decreased very slowly (0.07% per year), whereas the CPISP for paddy cropping decreased sharply (3.1% per year, on average) for the first two years and then remained steady during the following years. Therefore, upland crops in double-cropping systems consume the most inherent soil productivity, whereas paddy fields are favourable for maintaining a high level of CPISP. Overall, our results demonstrate a need to further improve China's CPISP3 values to meet growing productivity demands. 相似文献