不同周龄SPF级SD大鼠胰腺自发性病变的组织学观察

研究不同周龄SPF级SD大鼠胰腺自发性病变的种类及其病变发生率,为药物安全性评价提供背景资料。收集3年安评试验中11、19、31周龄试验对照组大鼠胰腺制作病理切片,光学显微镜下观察SD大鼠胰腺病变的种类及组织形态学特点,并统计其病变发生率。结果显示,大鼠胰腺主要出现了以下病变:①单核细胞浸润:11周龄大鼠该病变的总体发生率为0.6%,其中雌性为0,雄性为1.25%;19周龄大鼠该病变的总体发生率为1.0%,其中雌性为1.0%,雄性为1.0%;31周龄大鼠该病变的总体发生率为1.0%,其中雌性为0,雄性为1.96%。②腺泡细胞空泡变性:11周龄大鼠未观察到该病变的发生;19周龄大鼠该病变的总体发生率为1.0%,其中雌性为0,雄性为2.0%;31周龄大鼠该病变的总体发生率为3%,其中雌性为2.1%,雄性为3.9%。③腺泡细胞萎缩、腺管增生:11周龄大鼠未观察到该病变的发生;19周龄大鼠该病变的总体发生率为0.5%,其中雌性为0,雄性为1.0%;31周龄大鼠该病变的总体发生率为3.0%,其中雌性为1.0%,雄性为4.9%。结果表明,SPF级大鼠胰腺可发生单核细胞浸润、腺泡细胞空泡变性、腺泡细胞萎缩及腺管增生等自发性病变,且病变发生率可随着年龄的增加而增加。     

Change in the impression of rooms with interior wood finishes arranged differently: questionnaire survey with the use of photographs for the analysis of impressions of rooms concerning living activities

Efforts were made to quantify the impact of visual stimulation from interior wood finishes on the impression of room interiors, using feelings about living activities as indicators. Four types of room interiors with interior wood finishes used in different proportions were simulated. The room interiors were photographed from the same viewpoint. A survey was conducted by interviewing 200 test subjects. As a result of the analysis of principal components, it was found that desires of living activities obtained from the four photographs consisted of two factors: desires for calmness and desires for activities. A great desire for calmness was obtained from photographs showing wood materials. A weak desire for calmness and a strong desire for activities were obtained from a photograph that showed no wood materials. Great desires for both calmness and activities were obtained from a photograph showing wood materials used only as flooring material. As for an evaluation of “living,” the photograph of a room interior without wooden materials was evaluated as a place that test subjects did not feel like living in. The photograph showing wood materials used only as flooring material was evaluated as the place that test subjects most felt like living in, and considered most suitable as a living space.     

黄土丘陵区生态农业建设规划初探──彭阳县白岔村生态农业建设规划与实施效益

宁南彭阳县黄土丘陵半干旱区的白岔村，自１９７９年开始综合治理。“八五”开始依据生态学原理与系统工程的方法，进行了生态农业建设的规划设计，经过３年的实施验证，于１９９３年取得了显的生态经济效益，治理水土流失面积达到７０％，林草覆盖面积５０％，森林覆盖率１９％；人均有粮４００ｋｇ，人均纯收入４０８元。全村９７．２％的农户解决了温饱，有１３％的农户人均有粮超５００ｋｇ，纯收入超１０００元。     

Investigation into the use of the amylase SD assay of milled wheat extracts as a predictor of baked bread quality

α-Amylase content in milled wheat is a key quality parameter in the baking industry. This metric is usually determined using the Hagberg Falling Number (FN) method, the results of which can be used to segment grains into a range of quality categories. The authors describe herein an alternative to the traditional FN assay, namely the Amylase SD assay, as a predictor of baked bread quality. 144 Wheat grain samples with known FN values were analysed using the Amylase SD assay and a simple model for the conversion of Amylase SD units into “predicted FN” (PFN) values has been described. To test the usefulness of this approach, 11 separate wheat grain samples were analysed using the FN method, the Amylase SD assay, and also for gluten content. These grain samples were then milled to produce flour which was re-analysed using the Amylase SD assay and then formulated into dough that was subjected to Mixolab analysis, before being baked to produce pan bread. Correlations of baked bread volume and common Mixolab parameters to both FN and PFN values have been investigated for each sample. The Amylase SD assay has been shown to be a potentially promising alternative to the FN method for use as a baked bread quality predictor.     

盐酸双氟沙星对大鼠致畸胎试验

本试验研究了盐酸双氟沙星对ＳＤ大鼠的致畸性。剂量分别为３００ｍｇ／ｋｇ,ｂ．ｗ．;１５０ｍｇ／ｋｇ．ｂ．ｗ．和５０ｍｇ／ｋｇ ｂ．ｗ．,于大鼠受孕７至１５ｄ经口给药,３００ｍｇ／ｋｇ ｂ．ｗ．和１５０ｍｇ／ｋｇ．ｂ．ｗ．剂量的盐酸双氟沙星显著影响胚胎的生长发育,引起胎鼠死亡和骨骼畸形,其严重程度具有剂量效应关系。     

茶多酚对肥胖大鼠器官指数及腹脂的影响

将80只21日龄SD大鼠,按体质量相近原则分为普通对照组（Ⅰ组）、模型对照组（11组）、试验Ⅲ组、试验Ⅳ组、试验Ⅴ组和试验Ⅵ组,每组10只大鼠,试验期80d;普通对照组饲喂基础日粮,其他各组饲喂高能高脂目粮,40d后,试验Ⅲ、Ⅳ、Ⅴ、Ⅵ组分别灌服10、20、40、80mg／kg·d茶多酚,普通对照组与模型组灌服等体积生理盐水,至80d试验结束后禁食不禁水12h,逐只称重,剖检取腹脂（睾丸周围和后腹部脂肪）、心、肝、脾、肺、肾并称重。结果显示,在制模后,与普通对照组与模型对照组相比,试验Ⅲ、Ⅳ、Ⅴ、Ⅵ组腹脂重量差异极显著（P〈0．01）;灌服茶多酚试验组的大鼠各器官指数与对照组差异不显著（P〉0．05）。结果表明,茶多酚对肥胖大鼠器官指数无影响,对腹脂重量有抑制作用。     

大鼠子宫蜕膜基质细胞的体外培养试验

为摸索大鼠蜕膜细胞的培养方法,采用胰蛋白酶消化蜕膜组织,全组分蜕膜细胞培养,传代提纯蜕膜基质细胞,在4孔细胞培养板中直接进行泌乳素(PRL)免疫细胞化学检测培养细胞的组成.所采用的方法具有耗时少、细胞活率高的优点.结果:实验方法简单、经济、实用.     

基于SD法的图书馆阅览空间评价

运用心理学的相关理论与方法,研究读者在阅览空间内的阅读行为及读者对阅览室空间环境的感知情况,并将此研究结果反馈到图书馆的服务和管理中,为改善图书馆阅览空间环境的提出优化建议。     

大鼠不同部位内皮细胞的体外培养

为了建立一种能有效分离、培养和获取较高纯度的大鼠心肌膜微血管内皮细胞(RMMECs)、肠黏膜微血管内皮细胞(RIMECs)、主动脉内皮细胞(RAECs)、后腔静脉内皮细胞(RPVECs)的模型,自7dSD大鼠分别取左心室壁、空肠、主动脉、后腔静脉,采用II型胶原酶消化、差速贴壁、差速消化法获得较纯的RMMECs、RIMECs、RAECs、RPVECs。结果表明:通过形态学特征及微血管内皮细胞和血管内皮细胞相关特异性抗原检测证实,得到高纯度RMMECs、RIMECs、RAECs、RPVECs。结论:利用胶原酶II型消化法成功分离并培养大鼠的RMMECs、RIMECs、RAECs、RPVECs,为进一步开展内皮细胞的生物学特性的相关研究提供有效的方法。     

鸡传染性法氏囊病病毒SD株毒力测定

从一个免疫失败鸡场中分离到一株鸡传染性法氏囊病病毒野毒株,命名为SD株.经血清学试验、鸡胚接种、病毒形态观察等证实了分离物为鸡传染性法氏囊病病毒(IBDV).测定病毒效价ELD50达到10-6.5/0.2 mL;动物回归试验表明,该SD株接种4周龄鸡后引起鸡群发病率为100%,病死率达85%,剖检可见法氏囊呈"紫葡萄样...