利用重组自交系群体检测水稻耐铝毒数量性状基因座

利用Kinmaze / DV85 81个重组自交家系(RIL)作图群体,采用苗期单营养液水培鉴定方法,以相对根伸长量（RRE）作为耐铝毒性状的表型值,分析亲本和重组自交系群体对铝毒的耐性表现。利用Windows QTL Cartographer 1.13a软件共检测到5个耐铝毒QTLs,分别位于第1、5、8、9和11染色体上,各个QTL的贡献率在8.64%～18.60%之间,其     

Pre-maturity α-amylase and incipient sprouting in UK winter wheat,with special reference to the variety Rialto

In 1998 and 1999 the UK winter wheat variety Rialto produced unexpected low Hagberg falling numbers that could not be directly linked to sprouting. It was proposed that these reductions in quality could be due to pre-maturity α-amylase activity (PMAA). The problem was not identified during the selection and commercial development stages. Our study tested the hypothesis that the variety Rialto is PMAA-susceptible. Analysis was done on 13 year-location combinations of field grown Rialto. Together, visual and chemical assessments of sprouting and iso-electric focusing of α-amylase isozymes identified several samples with significant α-amylase activity in the apparent absence of sprouting. In addition, tests with α-amylase sensitive Phadebas gel revealed distinctive PMAA discoloration patterns in 10–44% of the grain from the 13 samples, leading to the conclusion that Rialto is PMAA-susceptible. Diurnal temperature range accumulated for an 11 day period during a warm spell in early simulated grain development displayed a significant but negative correlation with the number of grains showing clear PMAA discoloration patterns on Phadebas gel. The number of clear PMAA grains correlated positively with rainfall accumulated over 11 days. These results suggest that PMAA can increase under conditions similar to those conducive to pre-harvest sprouting. It is however also possible that in some instances both PMAA and incipient sprouting could have produced similar patterns of α-amylase activity. In addition to tests with Rialto, Phadebas gel tests were therefore also done with the known high Hagberg varieties Option and Malacca, sprouted in a controlled environment. Results from the additional gel tests suggest that visual and chemical assessments of sprouting in the grain combined with Phadebas gel analysis could identify PMAA more reliably in grain sub-samples than Phadebas gel analysis alone.     

亚麻籽油的开发利用

亚麻是山西省高寒山区的主要油用作物之一。亚麻籽含油率达到42%,油中不饱和脂肪酸占到主要脂肪酸组成的91%,其中,ω-3脂肪酸含量高达49%,比其他油用作物含量高7倍～50倍。ω-3脂肪酸营养油具有预防冠心病、心脑血管病,降低胆固醇,抗血栓,降血压,提高动脉系统柔性的功能,极具开发价值。ω-3脂肪酸营养油是在常规油脂加工工艺基础上,通过对部分工艺的改进,使产品中α-亚麻酸的含量较传统工艺提高了38.9%。     

Materials Hydrogen Storage V3TiNi0.56Al0.2 by Self-propagating High-temperature synthesis

With the help of the calculation of adiabatic temperature and thermodynamic analysis of the reactive system the autaurs discuss possibility about system of V 2O 5-TiO 2-Al by Self-propagating High-temperature synthesis(SHS).Hydrogen Storage V 3TiNi 0.56 Al 0.2 by SHS is studied by XRD,SEM and scanning electron microscopy.The experiment result indicates that it has same microstructure between synthesization by SHS and other ways.PCT curve indicated that alloy has certain hydrogen storage capability,saturated capacity of hydrogen storage is 0.092 L/g,which is a little lower than alloy by other ways.     

甘薯基因组NBS-LRR类抗病家族基因挖掘与分析

NBS-LRR类基因家族是植物抗病R基因(Resistance gene)数量最多的一类,具有NBS (Nucleotide-binding site)和LRR (Leucine-leucine-repeat)结构域。甘薯(Ipomoea batatas)栽培种基因组已完成测序,但尚未注释,本研究对甘薯基因组序列进行外显子预测,得到甘薯染色体组全基因组蛋白序列,在此基础上进一步对NBS-LRR家族基因鉴定和分析表明,甘薯基因组中含有379个NBS-LRR家族基因,占全基因组基因总数的0.212%,其中N型亚家族120个,NL型103个, CNL型133个, TNL型22个, PN型1个。所有染色体上均有NBS-LRR家族基因分布,但数量明显不同,其中有60.9%的NBS-LRR基因序列呈簇状分布。NBS-LRR基因序列有15个保守结构域,在N端较为保守。研究结果为甘薯进一步开展NBS-LRR家族基因的功能研究和抗性育种提供了参考。     

Isolation and Characterization of GhLEA3 Gene from Upland Cotton and Its Expression in Response to Low Temperature Stress

[Objective] Based on the analysis of cotton GhLEA3 gene structure and its expression model in chilling stress, we investigated the resistance function of GhLEA3 in response to cold stress. [Method] We cloned GhLEA3 from upland cotton by homologous sequence cloning way. We analyzed the properties of the GhLEA3, and constructed phylogenetic tree by bioinformatics analysis. We constructed the transient expression vector 35S∷GhLEA3-GFP by In-Fusion connection technology and studied the subcellular localization of GhLEA3. The expression of GhLEA3 gene in leaves of TM-1 at three-leaf stage under low temperature stress treatments (4 ℃, 24 h) was performed. Agrobacterium tumefaciens mediated floral dipping method was used to transform the gene with expression vector 35S∷GhLEA3-GFP into Arabidopsis thaliana wild type. Low temperature germination experiment at 4 ℃ was conducted to verify germination ability of the transgenic Arabidopsis thaliana T₃ seed. The transgenic Arabidopsis seedlings were treated at 4 ℃(low temperature), and all the leaves were taken to measure their electrical conductivity. [Result] The coding sequence of GhLEA3 gene is 1 218 bp, which encodes 405 amino acids. GhLEA3 protein includes 4 functional domains of PF02987. Phylogenetic analysis showed that the similarity of amino acid sequences between upland cotton GhLEA3 and Arabidopsis thaliana AtLEA3 was 52.6%. GhLEA3 might be mainly localized in vacuoles and small vesicles. GhLEA3 was up-regulated in leaves after low temperature treatment. The germination rate of transgenic Arabidopsis thaliana of T₃ generation at low temperature was significantly higher than that of wild type, and its electrical conductivity of leaves after low temperature treatment was significantly lower than that of wild type. [Conclusion] GhLEA3 belonged to the member of LEA3 family. Phylogenetic analysis showed that GhLEA3 had the closest relationship with AtLEA3. GhLEA3 was induced by low temperature stress and may play an important role in improving cold resistance.     

用转轮内三元流动计算对泵叶轮进行改型设计

在有厚翼栅奇点解法的基础上，利用一种考虑叶片厚度影响的叶片式水力机械转轮内准三元流动新的分析方法、准确计算了泵叶轮叶片表面上的速度与压力变化分布规律，获得了许多水力性能评估参数；通过评价这些参数，对叶片型线进行了修改，获得了比较满意的结果。为叶轮的改型设计提供了一条较有价值的新途径。     

采棉机传动部分虚拟装配过程的研究

用UG软件对采棉机传动部分零件建模。模型经过3ds max处理后,通过3ds max与nVsion平台两者之间的接口,将模型导入到虚拟现实仿真平台nVsion中,根据在3ds max中确定的装配关系,对传动部分的零件在nVsion虚拟场景下进行装配过程仿真,装配过程显示更加真实、清晰,并且平台实现了人机交互,找到了一种装配过程仿真的新方法。经过多次装配过程的比较,找出了最合理的装配工艺。     

三维平行微间隙流场数值模拟

为了研究上游泵送机械密封微间隙流体动压效应,将螺旋槽端面微间隙简化为三维直槽端面平行微间隙以排除泵送效应的影响.据此,建立了平底槽、渐扩台阶槽和渐缩台阶槽的平行微间隙液膜几何模型和计算模型,分别在槽板移动和平板移动的情况下针对不同移动速度和不同槽深进行流场数值模拟和开启力计算研究.研究表明：在文中研究参数范围内,无论是平底槽还是台阶槽,液膜收敛区出现高压,液膜发散区出现低压;液膜空化前,无论是槽板移动还是平板移动,平底槽微间隙开启力大小与平移速度、槽深、板间隙大小无关;液膜空化后,平底槽和台阶槽液膜开启力均随平移速度的增大或板间隙的减小而明显增大,但在同等条件下,槽板平移产生的开启力大于平板平移,且槽板平移时加大槽深能增大开启力,渐扩台阶槽平行微间隙能产生更大的开启力.