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11.
以八仙花的茎段、茎尖、叶片为外植体,在MS培养基中加入不同质量分数的6-苄基腺嘌呤(6-BA)和吲哚丁酸(IBA)进行增殖培养.结果表明:适宜八仙花外植体表面灭菌的方法是利用0.1%氯化汞(HgC l2)灭菌7 m in;不同类型的八仙花外植体在初代培养时的增殖效果不同,茎尖在培养过程中首先伸长生长,然后陆续长出侧芽,表明八仙花的茎尖是比较适合用来进行增殖培养的外植体;继代培养以MS+6-BA 1.0 mg.L-1+IBA0.12 mg.L-1为较适宜的增殖培养基;侧芽在1/2 MS+IBA 0.2 mg.L-1培养基中生根率和单苗根数量均较高,说明1/2 MS+IBA 0.2 mg.L-1为较好的不定根诱导培养基. 相似文献
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薄荷的快速繁殖与栽培技术的研究 总被引:3,自引:0,他引:3
本文介绍了薄荷的特性、栽培技术及用途,表明它在我们日常生活中起着重要的作用。用薄荷的组织培养方法可加速其繁殖系数,以达到迅速应用的目的。 相似文献
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By using RACE (rapid amplification ofcDNA ends) based homologous cloning strategy, we have successfully isolated the genomic and full-length cDNA sequences of a gene encoding typical DFR (dihydroflavonol-4-reductase) from black-seeded Brassica campestris L. var. oleifera DC.. The gene, designated BcDFR here, is 1 722bp in length and harbors 5 introns with typical splice sites of plant DFR genes. BcDFR cDNA is 1311bp in length with a 1 158bp ORF as well as a 25bp 5‘ UTR and a 128bp 3‘ UTR. The encoded BcDFR protein is 385 aa with a calculated Mw of 42.85kD and a pI value of 5.55. The nucleotide and amino acid sequences of this gene share extensive homologies to plant DFR genes of wide origins especially high similarities to Cruciferous DFR genes. Sequence analyses such as phylogenetic analysis, conserved domain search and substrate specificity region detection all indicated that BcDFR gene is a quite potentially biofunctional gene. Its cloning enables us to further dissect the possible relatedness between DFR gene and Brassica seed coat color traits and to create transgenic novel yellow-seeded rapeseed germplasm through antisense- or RNAi-suppression of DFR gene expression in black-seeded elite cultivars. 相似文献
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Additional time delay of arrival resulted from non-line of sight(NLOS)propagation and muti-path effect in(cellular) environment is the dominant factor resulting in TDOA location error,study on the statistical property of TDOA error is useful for improving the accuracy of TDOA location.Based on the exponential distribution property of NLOS propagation delay,and considering the system error resulted from the signal detection,the authors establish a statistical model of arrival time and a model of TDOA error distribution by statistics when non-light of sight and multi-path propagation exist.The models reflect the statistical property of arrival time and TDOA error in cell network,the simulation shows that the proposed models have a good accuracy and a satisfactory behavior. 相似文献
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Sheng Guangmin Gong Shihong Zhu Jinfu 《保鲜与加工》1989,(2):67-72
In this paper, the strain fatigue: crack propagation rates of vanadium bearing HSLA steel with different micro-structures are measured under constant load conditions, and the relationship between the crack propagation rate and the microstructures of steel is also studied. 相似文献
18.
观赏羽衣甘蓝的离体繁殖 总被引:5,自引:0,他引:5
以花蕾为外植体,离体繁殖羽衣甘蓝8个杂各一代材料。培养基为(mg/L):MS+BA1-2+NAA0.1-0.2+IAA0.5+(或不加)GA30.3+3%蔗糖。平均18%-67%的接咎花蕾产生不定芽;药有30%的不定芽发育成完整植物。培养物的严重褐化和再生植物的普遍玻璃质化是影响植株成活的主要因素。培养基中加入活性炭和青霉素,并给以强光照射,可提高植株成活率。 相似文献
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The factors influencing the decision of smallholder farmers to adopt new farming technologies were studied with reference to rubber–tea intercropping in Sri Lanka. Rubber–tea intercropping has been recommended previously to rubber farmers as a means to improve productivity and income during the early pre-tapping phase of rubber growth. Although crop trials have shown that the two crops are agronomically compatible and potentially produce a combined economic yield superior to the yield of a sole crop grown on the same area of land, there is little evidence of widespread adoption of this practice among smallholder farmers in Sri Lanka. The aim of the study was to determine the major factors that influence the decision to undertake rubber–tea intercropping and to construct a predictive model that describes the likelihood of adoption of intercropping by traditional smallholder rubber growers. A rapid rural appraisal (RRA) was undertaken based on semi-structured interviews of 90 smallholder farmers in the main rubber growing low wet zone of Sri Lanka. Among a number of factors shown to significantly influence the decision to intercrop tea with rubber, three were shown to operate independently, namely level of income, source of income (i.e. solely from own farm or from farm plus additional off-farm enterprises), and availability of land considered suitable for tea cultivation. A statistical model developed through correlation and logistic analysis, which predicts the likelihood of a smallholder adopting intercropping based on these factors, is presented and discussed. The most likely combination of circumstances (82% probability) under which rubber–tea intercropping is practiced is shown to be where the farmer’s income is greater than Rs. 10,000 per month, where the farmer’s income is based solely on own farm enterprises, and where more than 80% of the farmer’s land area was judged to be suitable for tea cultivation. Conversely, 30% of smallholder farmers that chose not to intercrop did possess land suitable for tea cultivation. Qualitative responses to the RRA indicated that limitation of technical knowledge was the main problem subsequently faced by rubber farmers who had adopted rubber–tea intercropping. Results indicate that there is need for both income support through farm subsidies and further agricultural extension services, if rubber–tea intercropping is to be adopted more widely in Sri Lanka. The wider usefulness of the developed logistic model in determining the likelihood of adoption of intercropping by smallholder farmers is discussed. 相似文献