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51.
芳樟工厂化育苗技术研究 总被引:10,自引:1,他引:10
通过对芳樟工业原料林良种茎尖的离体培养和繁殖 ,探讨芳樟茎尖诱导、分化、不定芽生长分化、生根的适宜的培养基和适宜的培养条件。结果表明 :适宜芳樟茎尖诱导培养基为MS +6 -BAa~ 2amg .L-1+NAAbmg .L-1,诱导率达 70 %以上 ,适宜分化丛生芽的理想培养基为改良MS +6 -BAa′~ 2a′mg .L-1+NAA0~ 2bmg .L-1+GA0~cmg .L-1芽年增殖系数达4 5 12 以上 ,有效芽苗率达 80 %以上 ,适合生根培养基为 1/2改良MS +NAAb′mg .L-1+IBA0~b′mg .L-1或 1/2改良MS +IBAb′mg .L-1+NAA0~b′mg .L-1,或R +IBAc2 mg .L-1+NAAc1 mg .L-1生根率达 98%以上。移栽土壤基质以黄心土加沙(10∶1)理想 ,移栽成活率可达 85 %以上 相似文献
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白玉兰离体培养和快速繁殖 总被引:6,自引:0,他引:6
试验建立了白玉兰离体繁殖体系。种子为外植体 ,初代培养基为 :B5 +BA0 .5mg/L +NAA0 .0 5mg/L + 30g/L糖 +琼脂 6g/L ,萌发率 85 %。继代培养基为 :B5 +BA1.5mg/L +NAA0 .15mg/L + 30g/glucose/sucrose + 6g/Lagar+Vco0 .5g/L ,月增殖率可达 5 .0± 0 .1。生根培养为 :1/2B5+NAA0 .5mg/L +AC1.0mg/L +VB2 0mg/L。以试管苗茎段、根段做外植体 ,培养基B5 +BA2mg/L+ 2 .4 -D0 .5mg/L +VC0 .5g/L ,可获得少量愈伤组织 ,继代存活率仅为 30 %。没有再分化成苗 相似文献
53.
驱蚊香草的组织培养和快速繁殖 总被引:4,自引:0,他引:4
以驱蚊香草幼嫩枝条为外植体,进行离体培养,获得再生植株,通过这个过程,得出有利于芽诱导、增殖、诱导生根的最佳配方,为驱蚊香草快速繁殖,规模化生产打下技术基础。 相似文献
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Ground-coverchrysanthemum(Dendranthemaxgrandof0raTzvel)isanexcellentpersistingrootflowerwithshortplant,highregressiveresistance,densebloomandcarelessmanagement.Itisre-gardedasagoodbreedforgardenaffores-tationandcomesintobloomaroundtheNationalDay.Cuttageandplantdivisionmethodsarethemostinuseforbreedingofthisspecies-Thetest-tubebreedingmeth-odwasad0ptedinthisexperimentinordertoincreasethebreedingc0efficientoftheimprovedbreed.MATERIALSANDMETHODSTheshort-yellowspeciesofground-coverchrysa… 相似文献
56.
Kasper Johansen Stuart Phinn Ian Dixon Michael Douglas John Lowry 《Forest Ecology and Management》2007,240(1-3):42-60
Suitable methods for measuring and monitoring the condition of riparian environments are being investigated by government agencies responsible for maintaining these environments in Australia. The objective of this work was to compare two riparian condition assessment approaches, the Tropical Rapid Appraisal of Riparian Condition (TRARC) method developed for rapid on-ground assessment of the environmental condition of savanna riparian zones and an image based riparian condition monitoring scheme. Measurements derived from these two approaches were compared and correlated. The sample representativeness of the TRARC method was evaluated and the cost-effectiveness and suitability for multi-temporal analysis of the two approaches were assessed. Two high spatial resolution multi-spectral QuickBird satellite images captured in 2004 and 2005 and coincident field data covering sections of the Daly River in the Northern Territory, Australia were used in this work. Both field and image data were processed to map indicators of riparian zone condition including percentage canopy cover, organic litter on the ground, canopy continuity, tree clearing, bank stability, and flood damage. Spectral vegetation indices, image segmentation, and supervised classification were used to produce riparian health indicator maps. QuickBird image data were used to examine if the spatial distribution of TRARC transects provided a representative sample of ground based estimates of riparian health indicators. Covering approximately 3% of the study area, the sample mean of the TRARC estimates of individual indicators of riparian zone condition were in most cases within 20% of the global mean derived from the whole imaged riparian area. The cost-effectiveness of the image based approach was compared to that of the ground based TRARC method. Results showed that the TRARC method was more cost-effective at spatial scales from 1 km to 200 km of river in relatively homogeneous riparian zones along rivers with only one channel, while image based assessment becomes more feasible at regional scales (200–2000 km of river). A change detection analysis demonstrated that image data can provide detailed information on gradual change, while the TRARC method is less suited for multi-temporal analysis due to the ranked data format, which inhibits precise detection of change. However, results from both methods were considered to complement each other for single date assessment of riparian zones if used at appropriate spatial scales. 相似文献
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为了探究光照处理对中国被毛孢生长代谢的影响,通过分离单子囊孢子获得单孢菌株,并对该菌株进行光照处理。观察记录光照处理30 d后菌落和菌丝形态的变化,测量菌落鲜质量、干质量以及分生孢子量,通过比色法检测甘露醇、多糖、尿素和多酚4种代谢物含量的变化,并通过UPLC检测腺苷含量的变化。结果表明,光照处理后中国被毛孢菌落形态发生显著变化;菌落鲜质量显著低于黑暗处理组,干质量无明显差异;菌丝的延伸被抑制并出现扭结现象;分生孢子数量显著高于黑暗处理组;多糖和尿素含量均显著低于黑暗处理组;甘露醇和多酚含量无显著变化;腺苷含量显著高于黑暗处理组。 相似文献