恩施与国外白肋烟中性致香成分含量的差异比较

为明确恩施白肋烟中性致香成分组分和含量及与国外白肋烟的异同,以恩施不同产区及美国、马拉维白肋烟样品为材料,采用气质联用仪对样品的中性致香成分含量进行分析测定,结果表明:恩施白肋烟中性致香成分总量及各组分含量在产地之间存在一定差异,其中香叶基丙酮、苯甲醛、糠醇等单一致香成分含量的变异较大。以巨豆三烯酮为代表的类胡萝卜素类降解产物含量以美国烟叶最高,恩施次之,马拉维最低;恩施白肋烟苯丙氨酸类、棕色化反应产物含量均高于美国和马拉维,而西柏三烯的主要降解产物茄酮则低于国外烟叶。聚类分析将供试样品分为3类,80%的恩施样点与美国同属一类,表明恩施白肋烟中性致香成分含量与美国具有较高的相似性。     

How do timing,duration, and intensity of drought stress affect the agronomic performance of winter rye?

Winter rye (Secale cereale L.) will be especially affected by drought induced yield losses in Central and Eastern Europe in the future because it is predominantly cultivated on low-fertile soils with a poor water-holding capacity. In order to examine the performance of winter rye under different drought conditions, field experiments were carried out during the years 2011, 2012, and 2013 near Braunschweig, Germany. Two sets of genotypes were tested under severe, mild, pre-anthesis, and post-anthesis drought stress in rain-out shelters as well as under rainfed and well-watered conditions. The grain, straw, and total above ground biomass yields, harvest index, grain yield components, leaf area index (LAI), and phenological characteristics were examined, as well as phenotypic correlations between grain yield and further characteristics. Drought induced grain yield reduction ranged from 14 to 57%, while straw yield and harvest index were lesser affected by drought than the grain yield. Under drought conditions, fully ripe was reached up to twelve days earlier than under non water-limited conditions. Pre-anthesis drought mainly reduced spikes m⁻² and kernels spike⁻¹ while drought during grain filling reduced the 1000-kernel weight (TKW) only. The grain yield was positively associated with straw yield, spikes m⁻², and kernels spike⁻¹ under water limited conditions while the TWK was only positively associated with grain yield under drought during grain filling. Consequently, high pre-anthesis biomass as well as high numbers of spikes m⁻² and kernels spike⁻¹ are especially important for obtaining high grain yields under water-limited conditions. Focusing on these traits is, therefore, recommendable for developing drought tolerant rye genotypes.     

巴西橡胶树乳管细胞HblMYC3互作蛋白筛选与功能分析

MYC是b HLH转录因子家族的亚家族成员,在植物茉莉酸信号转导过程中发挥着重要的调节作用。Hbl MYC3是从巴西橡胶树的乳管细胞中分离鉴定到的MYC类转录因子,其基因表达受割胶和茉莉酸上调。采用酵母双杂交方法初步筛选Hbl MYC3蛋白的互作蛋白,旨在进一步了解Hbl MYC3的功能。结果表明:Hbl MYC1、Hbl MYC2、DNAJ蛋白、谷氧还蛋白2、含A20和AN1锌指结构域的胁迫相关蛋白5、28 ku热和酸稳定的磷蛋白以及25 ku泛素连接酶E2等7种蛋白不同程度地与Hbl MYC3蛋白互作。基于这些互作蛋白的功能,推测Hbl MYC1或Hbl MYC2通过与Hbl MYC3形成二聚体对小橡胶粒子膜蛋白基因表达进行调控,其他蛋白参与胁迫条件下维持二聚体的稳定性和胁迫反应后降解该二聚体。     

浅议通过标签内容查找农药隐性成分

本文针对农药隐性成分危害大和监管难等问题,剖析了企业主要动机,以农药标签为对象,研究建立了一种查找"问题"农药、锁定农药隐性成分的方法。该方法具有成本低、易于普及、克服检测仪器谱库不足、发挥基层各方力量进行监管等优点。与检测技术结合使用,可以弥补各方不足,更好地发挥监管作用。     

油菜籽来源抗氧化物质研究进展

油菜籽中含有多种具有抗氧化活性的物质，为抵御机体的氧化损伤起到了积极作用。本文综述了油菜籽来源的诸多抗氧化活性成分及抗氧化活性的评价方法。油菜籽主要的抗氧化成分有肽类、多酚类、多糖类、生育酚和其它抗氧化成分，其中的肽类主要有三种产生方式：非消化酶酶解、消化酶酶解和发酵。针对油菜籽来源的活性物质抗氧化活性的评价方法，包括体外测定方法（化学检测）和细胞内抗氧化实验和动物实验。综述还探讨了油菜籽抗氧化活性成分研究中存在的问题和解决方法，旨在为油菜籽活性成分的开发和利用提供参考。     

高尔夫球手地方依恋符号性元素的识别与运用

运用隐喻抽取技术对高尔夫球手地方依恋的符号性元素进行了识别与定量研究。结果表明,高尔夫球手地方依恋有22个符号性元素,这些元素可分为风格、地理、参与、接待和文化五类,它们给球手带来"友谊"、"健康"、"愉悦"、"品质高"、"风景美"的终极体验。经营者要充分利用符号性元素的意义,着重打造球场基础设施、园林景观、服务接待和球会文化;积极挖掘联结构念的耦合价值,实现球手与球场的符号互动;主动创设高尔夫球手游憩氛围情境,避免球手地方依恋的中断或转移;结合球会自身特点,适时进行符号化运作。     

SPME/GC-MS法分析不同龙眼品种果实中的香气成分

采用 SPME/GC-MS法对4个龙眼品种果实香气成分进行了分析鉴定。结果表明，4个龙眼品种共检测出44种芳香物质，其中烷类11种，烯类18种，酯类10种，醇类3种，酮类1种，炔类1种，它们构成4种龙眼主要的香气成分。4个龙眼品种在香气组成和含量上有所差异，储良、东良、东丰、石硖中分别含有25种、24种、26种、21 种香气成分。其中，4种龙眼共有的香气成分有7种：分别是十七烷、罗勒烯(顺式)、罗勒烯(反式)、别罗勒烯、1,3,8-对-薄荷三烯、α-石竹烯、(E)-β-金合欢烯，但其相对含量都有所差异；此外，各品种也具有自己独特的香气成分，如储良特有的香气成分有9种：包括十六烷、2-甲基-4-亚甲基-5 -(2,2-二甲基环丙基)-1-戊烯、5-环丙基戊酸乙酯、二十酸乙酯、棕榈酸乙酯、9-十六碳烯酸乙酯、香叶基芳樟醇、芳樟醇、2,7-二甲基-3-辛烯-5-炔；东丰特有的3种：包括1-碘十一烷、反式，反式-法尼基酸甲酯、2-羟基十二烷酸甲酯；石硖特有的4种：包括十一烷、(-)-异丁香烯、1,3,3-三甲基-2-乙基环己烯、喇叭茶醇；东良没有特有的香气成分。     

Phenotypic and allelic variation for wort protein Z in Australian and Canadian barleys

Protein Z is a major component in beer foam. Two-dimensional electrophoresis was used to analyze wort proteins of two Australian (Buloke and Commander) and two Canadian (CDC Meredith and Bentley) varieties. The Canadian barley contained more abundant proteins from MW 40–45 kDa (pI 5 to 7). These proteins were identified as either protein Z4 or protein Z7 using liquid chromatography–mass spectrometry. Full-length gene of protein Z4 and Z7 were sequenced from Canadian and Australian barleys. Sequence differences were identified in the coding region and upstream regions of the two genes, resulting in protein sequence and expression variations. Molecular markers were designed according to the indels in the upstream regions of protein Z4 and Z7 genes. These markers were highly correlated to wort protein Z content in Canadian and Australian varieties. The Canadian barleys contained ‘high level’ genotypes for protein Z4 and Z7 while most Australian barleys had ‘low level’ genotypes for protein Z4, Z7 or both. The markers identified in this study provide a valuable tool for the selection of protein Z alleles in marker-assisted breeding. Total protein Z content was assessed using different steeping conditions, and increasing air-rest time increased protein Z content in 15 varieties.     

Effect of germination time and temperature on the functionality and protein solubility of sorghum flour

Sorghum was germinated for different time (12, 24, 36 and 48 h) at different temperatures (25, 30 and 35 °C) and the changes in their nutritional and functional properties of germinated sorghum flour were assessed and compared with native sorghum flour. Germination inversely affects the crude protein, fat, fibre and ash content. A decrease in water absorption and swelling power and increase in oil absorption capacity was observed due to enzymatic starch modification as the germination duration progressed. Germination of sorghum increased the gel consistency while paste clarity was decreased as compared to native flour. Proteins were modified by action of enzymes during higher germination time and temperature conditions, which results in significantly higher protein solubility of germinated sorghum flour, which also result in enhancing the foaming and emulsifying properties of the flour. Lowest % synersis value and least gelation concentrations were observed in native sorghum has, which increased during germination and were highest in sorghum germinated for 48 h at 35 °C. Germination in overall can be used as low cost natural bio-processing technique for the preparation of modified flour with enhanced function properties without chemical modification or genetic engineering.     

黑曲霉菌渣资源化再利用途径分析

黑曲霉菌渣是黑曲霉发酵法生产葡萄糖酸钠后产生的废弃物，其资源化利用已成为企业扩大生产亟需解决的问题。对该菌渣有效成分检测得知，该菌渣有害金属元素均小于城镇垃圾农用控制标准、有机-无机复混肥料控制标准和鱼粉、猪、家禽添加剂预混合饲料卫生指标，有机质含量符合有机肥料中的有机质含量指标，总糖、粗纤维、粗蛋白、脂肪等营养成分含量较多，同时也含有丰富的N、P、K、Ca、Mg、Fe等元素，可从有机肥料和禽畜饲料方向进行资源化利用，有利于减轻环境污染及实现变废为宝，具有积极的社会、经济和生态效益。