福建省大雾分区客观预报方法研究

通过统计福建省大雾天气的气候特征以及成雾的气象要素特征,总结出成雾的气象要素指标。基于福建省中尺度数值预报模式产品,利用完全预报（PPI）方法,建立由降水、相对湿度、风向、风速、逆温等因子的预报概率方程,对大雾分区进行了客观预报。     

花卉抗寒性研究进展

综述了低温胁迫下花卉体内生理生化的变化规律,阐述了花卉抗寒性的机理,并对未来花卉抗寒性研究进行了展望。     

多媒体CAI技术在高校足球教学中的应用研究

通过多媒体CAI课件进行足球教学的实验研究,探讨多媒体技术在提高体育技术课教学效果和教学质量中的作用,通过文献资料法、对比实验法、问卷法和数理统计法进行研究,结果显示,学生在学习成绩,学习的积极性、主动性等方面差异显著,实验班的技术成绩明显好于对照班,表明这种教学方法能有效提高体育课的教学效果,发展前景良好。     

嘉庚蛸雄性生殖系统组织学观察

应用组织切片技术对浙江沿海自然分布的嘉庚蛸雄性生殖系统的结构变化进行了逐月研究,结果发现,雄性生殖系统由单个精巢、输精管、储精囊、前列腺、精荚囊和阴茎等6部分构成。精巢球形,辐射型精巢,精原细胞椭圆或近圆形,直径10～16μm,精子细胞经过椭圆形梭型柳叶型长棒型的变态期,成为鞭毛型精子,精子头部细长等粗棍状,长约26～33μm,尾部长度约为头部的16倍。输精管长约13～16cm,是雄性生殖系统中最细长的管道。储精囊长约5～8cm,可分为储精管和腺体2部分。前列腺与储精囊相接共同开口于精荚囊腔,中空盲管状,4.8～5.6cm。精荚囊分为2部分,一侧为叶脉状规则褶皱突起围成的腺体腔,称之为囊内腺,另一侧突起少,个体大、不规则,树枝状。阴茎短,仅2～3cm,生殖孔开口于胴体左侧外套腔的中部。储精囊、前列腺和精荚囊中均存在着具有分泌功能的上皮构成的腺体腔,它们在精荚成熟装配过程中发挥着不可取代的作用。阴茎中段的阴茎囊呈镜面倒卜型,它不仅是分泌性的腺体腔,而且还是精荚排出过程中的反转场所。     

Valuing Potential Benefits of Research into the Biological Control of Californian Thistle （Cirsium arvense）： A Bioeconomic Model

The present research developed a bioeconomic model for control of Californian thistle （Cirsium arvense L. Scop.） in intensive, lowland sheep pastures in New Zealand. Production costs included two control methods： herbicides and defoliation, with defoliation encompassing both physical means and biocontrol measures. The model was used to examine the economic value of a research programme into biological control of the weed, given several different future scenarios. These scenarios were high herbicide prices, a price premium for lamb raised without chemical herbicides, and development of herbicide resistance. The model results were used to estimate national impacts. If the only change in the agricultural sector was the cost of the biocontrol to the nation＇s farmers, the research programme to find an effective biocontrol agent had little economic impact. If other factors changed, such as the price of petroleum or levels of herbicide resistance in the weed. the model＇s results suggested that intensive sheep farming could grow or shrink by as much as NZ$219 million dollars over five years. In the latter case, having an alternative control method reduced potential losses and increased potential gains, resulting in a net benefit of between NZ$81 million and NZ$153 million. This work extended prior research in several ways. First. Californian thistle reproduces via root buds, which affected the modelling of weed behaviour. Secondly, the model allowed optimistation over continuous levels of weed control for two different methods. Thirdly, the model accounted for both weed control and damage to nitrogen-fixing clover from herbicide use. Finally, the research investigated optimal weed control in several different alternative future states.     

新疆生产建设兵团土地利用规划及效益分析

土地利用及其规划反应了社会经济发展状况和人类利用土地的方向及价值取向.通过对新疆生产建设兵团2010年土地利用规划的数据进行分析,由2000～2010年的数据验证该规划的可行性,在验证中采用分段年平均增长法系统的分析了2010年土地利用规划可能产生的生态、经济、社会效益.分析结果表明新疆生产建设兵团2010年土地利用规划为新疆生产建设兵团有目的、有秩序的发展提供了科学的依据,为决策者们提供了有力的理论基础.该规划为新疆生产建设兵团发展提供了理论依据,同时对新疆维吾尔自治区农业经济效益的整体提高也具有重要的战略意义.     

Study on Antimicrobial and Antiviral Activities of Lysozyme From Marine Strain S-12-86 In Vitro

In this study, the in vitro antimicrobial and antiviral activities of the lysozyme from marine strain S-12-86 （LS） were investigated, The antimicrobial activity of LS was tested by minimum inhibition concentration （MIC） and minimum bactericidal concentration （MBC） method. The inhibiting effects of LS on pseudo rabies virus （PRV） in swine kidney cells （PK-15 ceils） were judged by cytopathogenic effect test （CPE）, The results showed LS had a broad antimicrobial spectrum against several standard strains including gram-positive bacteria, gram-negative bacteria, fungi, etc, The MIC of LS was 0.25-4.00 mg mL^-1 and its MBC was 0.25-8.00 mg mL^-1, respectively, Observation under the transmission electron microscope revealed that the cell wall of Candida albicans was distorted seriously, and the cytoplasm with many cavities was asymmetrical after being hydrolyzed by LS, The median cytotoxicity concentration （TC50） of LS was 100.0 μg mL^-1, the median effective concentration （EC50） was 0.46 μg mL^-1, and the selectivity index （TI = TC50/EC50） was 217. LS could inhibit PRV in PK-15 cells when it was added to cell culture medium at 0, 2, 4, 6, and 8 h after PK-15 cells had been infected by PRV. From the results, we concluded that LS had broad antimicrobial spectrum and good inhibiting effects on PRV,     

DEAE-Sepharose Fast Flow分离纯化刺芹侧耳木质素降解酶

[目的]为Pleurotus eryngii—Co60-7木质素降解酶的分离纯化和综合利用提供试验依据。[方法]采用DEAE—Sepharose^TM Fast Flow离子交换介质,分别考察缓冲液pH值、流速和洗脱方式等对刺芹侧耳木质素降解酶分离纯化的影响,确定了最佳分离纯化层析条件。[结果]DEAE-Sephalose^TM Fast Flow分离纯化Pleurotus eryngii-Co60-7木质素降解酶的最佳层析条件为：选择20mmol／L,pH值为5．0醋酸钠一醋酸缓冲体系,3ml／min的流速,进行分步洗脱（100、200～300和1000mmoL／L NaCl的三步洗脱）,可较好地实现刺芹侧耳发酵液木质素降解酶初分,该纯化操作目标蛋白回收率达85％,纯化分离因素为2．71。[结论]该技术在分离纯化刺芹侧耳木质素降解酶上可行,具有潜在的工业应用价值。     

水杨酸分光光度法测定水中的NO_3~-

以水杨酸为显色剂,建立分光光度法测定水中NO3-含量的方法。结果表明,NO3-含量的最佳测定条件为：吸收波长410nm,1.0ml浓硫酸,1.0ml20% NaOH溶液,显色时间20min。此条件下,NO3-含量在0.5～8.0mg/L范围内遵循比尔定律。NO3-的最低检出限为0.1mg/L,加标回收率为98.0%～102.0%。     

鸡γ-干扰素与新城疫病毒F蛋白抗原表位融合基因的构建与表达

[目的]研究鸡γ-干扰素（ChIFN-γ）的免疫佐剂功能,构建ChIFN-γ与新城疫F蛋白抗原表位（NDV F2-3）的融合基因,并进行原核表达。[方法]以头尾相连的方式构建鸡γ-干扰素基因与NDVF2-3的pET-32a重组质粒,经PCR扩增、双酶切和测序鉴定后,重组子在E.coliBL21细胞进行IPTG诱导融合蛋白表达,采用SDS-PAGE和Western blot方法分别检测表达的产物。[结果]获得了726 bpChIFN-γ与NDVF2-3融合基因,经原核表达,融合蛋白分子量约为35 000,能与相应的抗体结合。[结论]ChIFN-γ与NDVF2-3串联基因在原核细胞中能有效表达,且融合蛋白具有一定的免疫活性。